LOCUS BBa_K325108 2891 bp DNA linear 17-May-2024 DEFINITION EPIC Firefly Luciferase (under pBAD)
P. Pyralis
(E. coli optimised) ACCESSION BBa_K325108 VERSION BBa_K325108.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 CDS 1236..2885 /label=EPIC luciferase (Photinus pyralis) RBS 1219..1235 /label=rbs misc_feature 2886..2891 /label=stop misc_feature 1219..2891 /label=BBa_K325101 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtt aaggaggtaa aaaaaatgga ggacgcgaaa aacatcaaga 1261 aaggcccagc accgttctat ccgctggaag atggtaccgc cggtgaacaa ttgcacaaag 1321 cgatgaaacg ttatgcgctg gttccgggta ccatcgcatt cacggacgcg catatcgagg 1381 ttaacatcac ctatgccgag tacttcgaga tgagcgttcg tctggccgag gcaatgaagc 1441 gttatggttt gaataccaat catcgcatcg tcgtctgtag cgagaacagc ttgcagtttt 1501 tcatgccagt tctgggtgca ctgtttattg gtgtggcagt ggcgcctgcg aatgatatct 1561 acaacgagcg tgaactgttg aatagcatga acatttcgca gccgacggtt gtttttgtgt 1621 ccaagaaagg tttgcagaag attctgaatg tgcaaaagaa gttgccgatc attcagaaaa 1681 tcatcatcat ggacagcaag accgattacc agggtttcca aagcatgtac acttttgtga 1741 cgagccacct gcctccgggt tttaacgagt atgatttcgt tccggaaagc ttcgaccgtg 1801 ataagacgat tgcactgatt atgaactcgt ccggctctac cggcctgcct aaaggcgtgg 1861 ccctgccgca ccgtactgca tgtgtccgct tcagccacgc gcgtgatccg atcttcggta 1921 accaaatcat tccggacacc gccattttgt ctgtcgttcc gtttcatcat ggctttggta 1981 tgtttaccac tctgggttac ctgatctgtg gtttccgtgt tgttctgatg taccgtttcg 2041 aagaagaact gttcctgcgt agcctgcaag attacaagat ccaatccgcg ctgctggtgc 2101 cgacgctgtt tagcttcttc gctaaatcca ccctgattga caaatatgac ttgagcaacc 2161 tgcacgaaat cgcgtcgggc ggtgcgccgc tgagcaaaga agtcggtgag gccgttgcga 2221 aacgtttcca tctgccgggc atccgtcagg gttatggcct gacggaaacg accagcgcga 2281 ttttgattac ccctgagggt gacgataaac cgggtgccgt tggcaaggtg gtaccgttct 2341 ttgaggctaa agtcgtggac ctggacacgg gtaagactct gggtgtgaat caacgtggcg 2401 agttgtgcgt tcgtggcccg atgatcatga gcggttatgt caacaacccg gaagcaacta 2461 atgcgttgat cgataaggat ggctggctgc acagcggcga cctggcgtac tgggatgagg 2521 atgaacactt tttcatcgtt ggtcgcctga agagcctgat caagtacaag ggctaccagg 2581 tggcaccggc tgaactggag agcattctgt tgcaacaccc taatatcttc gacgcgggtg 2641 ttgcgggtct gcctgatgat gatgccggtg aattgccagc tgcggtggtc gttctggaac 2701 acggtaaaac gatgactgaa aaagaaatcg tggactacgt cgcctcgcag gtgaccaccg 2761 ctaaaaagtt gcgtggtggc gtggtgtttg tggatgaagt gccgaaaggc ctgacgggta 2821 aacgtgatgc acgtaagatt cgtgagatct tgatcaaggc gaagaaaggt ggtaaatcca 2881 agttgtaata a //