LOCUS BBa_K325109 3844 bp DNA linear 17-May-2024
DEFINITION EPIC Firefly Luciferase and LRE (under pBAD)
P.
Pyralis
(E. coli optimised)
ACCESSION BBa_K325109
VERSION BBa_K325109.1
FEATURES Location/Qualifiers
promoter 1080..1210
/label=Pbad
promoter 1043..1114
/label=PC
misc_feature 1..1079
/label=AraC
promoter 1..1210
/label=BBa_I0500
RBS 1219..1235
/label=rbs
CDS 1236..2159
/label=Luciferin regenerating enzyme (P.pyralis)
misc_feature 2160..2165
/label=double stop codon
misc_feature 2166..2171
/label=BamH1 site
RBS 2172..2188
/label=rbs
misc_feature 2189..3838
/label=EPIC luciferase (P. pyralis)
misc_feature 3839..3844
/label=double stop codon
misc_feature 1219..3844
/label=BBa_K325100
ORIGIN
1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
1201 ttgggctagc tactagagtt aaggaggtaa aaaaaatggg tccggtcgtt gaaaagatcg
1261 ccgagctggg taagtacacg gttggtgagg gtccgcactg ggaccacgaa acgcagacgc
1321 tgtatttcgt ggatacggtc gagaaaacgt tccacaaata cgttccgagc cagaagaaat
1381 acacgttttg taaggtggac aagctggtta gctttatcat tccgctggca ggttctccgg
1441 gtcgcttcgt cgtcagcttg gagcgcgaga ttgcgatcct gacgtgggac ggcgtcagcg
1501 cggcaccgac cagcattgag gcgattgtga atgtcgagcc gcacatcaag aataatcgcc
1561 tgaacgacgg taaagcggac ccgctgggta atctgtggac cggcacgatg gccattgatg
1621 cgggtttgcc ggtgggtccg gtgaccggct ccctgtacca cctgggtgcg gataagaagg
1681 tcaagatgca tgaaagcaat attgccatcg ctaacggcct ggcgtggagc aacgacctga
1741 agaagatgta ctatattgac agcggcaaac gtcgtgttga cgaatacgac tatgatgcga
1801 gcacgctgtc tatcagcaat cagcgtccgc tgttcacctt cgaaaaacat gaggtccctg
1861 gctacccaga cggtcaaacc attgacgaag aaggtaacct gtgggtcgcc gtgtttcaag
1921 gccagcgtat tatcaagatt agcacccagc agccggaggt tctgctggat accgtcaaaa
1981 tcccggaccc gcaggtgact agcgttgcat tcggtggtcc gaatctggac gagctgcatg
2041 tgaccagcgc cggtttgcag ctggatgaca gctccctgga caaatctttg gtcaatggtc
2101 acgtctaccg tgtgactggc ctgggtgtta agggtttcgc cggtgtgaaa gttaagctgt
2161 aataaggatc cttaaggagg taaaaaaaat ggaggacgcg aaaaacatca agaaaggccc
2221 agcaccgttc tatccgctgg aagatggtac cgccggtgaa caattgcaca aagcgatgaa
2281 acgttatgcg ctggttccgg gtaccatcgc attcacggac gcgcatatcg aggttaacat
2341 cacctatgcc gagtacttcg agatgagcgt tcgtctggcc gaggcaatga agcgttatgg
2401 tttgaatacc aatcatcgca tcgtcgtctg tagcgagaac agcttgcagt ttttcatgcc
2461 agttctgggt gcactgttta ttggtgtggc agtggcgcct gcgaatgata tctacaacga
2521 gcgtgaactg ttgaatagca tgaacatttc gcagccgacg gttgtttttg tgtccaagaa
2581 aggtttgcag aagattctga atgtgcaaaa gaagttgccg atcattcaga aaatcatcat
2641 catggacagc aagaccgatt accagggttt ccaaagcatg tacacttttg tgacgagcca
2701 cctgcctccg ggttttaacg agtatgattt cgttccggaa agcttcgacc gtgataagac
2761 gattgcactg attatgaact cgtccggctc taccggcctg cctaaaggcg tggccctgcc
2821 gcaccgtact gcatgtgtcc gcttcagcca cgcgcgtgat ccgatcttcg gtaaccaaat
2881 cattccggac accgccattt tgtctgtcgt tccgtttcat catggctttg gtatgtttac
2941 cactctgggt tacctgatct gtggtttccg tgttgttctg atgtaccgtt tcgaagaaga
3001 actgttcctg cgtagcctgc aagattacaa gatccaatcc gcgctgctgg tgccgacgct
3061 gtttagcttc ttcgctaaat ccaccctgat tgacaaatat gacttgagca acctgcacga
3121 aatcgcgtcg ggcggtgcgc cgctgagcaa agaagtcggt gaggccgttg cgaaacgttt
3181 ccatctgccg ggcatccgtc agggttatgg cctgacggaa acgaccagcg cgattttgat
3241 tacccctgag ggtgacgata aaccgggtgc cgttggcaag gtggtaccgt tctttgaggc
3301 taaagtcgtg gacctggaca cgggtaagac tctgggtgtg aatcaacgtg gcgagttgtg
3361 cgttcgtggc ccgatgatca tgagcggtta tgtcaacaac ccggaagcaa ctaatgcgtt
3421 gatcgataag gatggctggc tgcacagcgg cgacctggcg tactgggatg aggatgaaca
3481 ctttttcatc gttggtcgcc tgaagagcct gatcaagtac aagggctacc aggtggcacc
3541 ggctgaactg gagagcattc tgttgcaaca ccctaatatc ttcgacgcgg gtgttgcggg
3601 tctgcctgat gatgatgccg gtgaattgcc agctgcggtg gtcgttctgg aacacggtaa
3661 aacgatgact gaaaaagaaa tcgtggacta cgtcgcctcg caggtgacca ccgctaaaaa
3721 gttgcgtggt ggcgtggtgt ttgtggatga agtgccgaaa ggcctgacgg gtaaacgtga
3781 tgcacgtaag attcgtgaga tcttgatcaa ggcgaagaaa ggtggtaaat ccaagttgta
3841 ataa
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