LOCUS BBa_K325209 3841 bp DNA linear 25-Nov-2024 DEFINITION Green Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised) ACCESSION BBa_K325209 VERSION BBa_K325209.1 FEATURES Location/Qualifiers misc_feature 1..1210 /label=I0500 misc_feature 1..1079 /label=AraC promoter 1043..1114 /label=PC promoter 1080..1210 /label=Pbad RBS 1219..1235 /label=rbs CDS 1236..2162 /label=Luciferin Regenerating Enzyme (L. cruciata) misc_feature 2163..2168 /label=double stop codon misc_feature 2169..2174 /label=BamH1 site RBS 2175..2191 /label=rbs CDS 2192..3835 /label=Wild Type Luciferase (L. cruciata) misc_feature 3836..3841 /label=double stop codon ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtt aaggaggtaa aaaaaatggc cccgaccgtg gaacaaattg 1261 tggagctggg tacgtatctg ctggccgaaa gcccgcactg ggacgatgaa acgcaaagcc 1321 tgtatttcgt cgatatcgtt ggtcgcagcg tgaacaaata cgttccgacg accaagacgc 1381 atacccaact gaaattcgac aagaatccga gcttcatcat cccggtgaag ggttgttccg 1441 accgttttat cgtcagcctg gaacgcgaga tcaacctgct gacctgggac ggcgcatcct 1501 ctgcaccatc caagattgag aagatcgcgg ttttcgataa tacgccagag aagagcgaaa 1561 accgtctgaa cgatggtaag gcagatccgc tgggcaacct gtgggcgggt accatgaata 1621 tgggttctga ccataccacc ggcacgccgg tgcgcggtac cctgagcagc ctgagcaaca 1681 agcaagtcaa agagcacgtc agcgaggtct gcatcagcaa tggcctggca tggagcaagg 1741 acctgaagaa attctattac attgatagcg ctgttcgtca agttgaccag ttcgatttcg 1801 atgcgaaaaa cctgagcttg agcaatcgtc aaccgctgtt cacctttgac aaacacggca 1861 tcatgggtag cccggatggt cagaccatcg atgcggaggg taacctgtgg gttgccacct 1921 gtcagggcga taaagtcctg aagattgata cttctacccc ggaaaccctg ctgggtattg 1981 tggagattcc ggaacatcag gtgacgagcg tttgcatcgg cggtgcagaa ctgaacgttt 2041 tgtatgtgac gaccgcgtcc attaaactgc ctggtgcgga tgaaaccaag ccgatgaaag 2101 gcgccatcta taaagtcacc ggtctgggtg tcaaaggtct gccgggtgac cgtgttaagc 2161 tgtaataagg atccttaagg aggtaaaaaa aatggagaac atggagaacg acgagaatat 2221 tgtggttggt ccgaaaccgt tctacccgat cgaagagggc agcgcgggta cccagctgcg 2281 taaatacatg gaacgctacg cgaaactggg tgcgatcgcg ttcacgaatg cggttaccgg 2341 cgtggactat tcctatgcgg agtatttgga aaagtcgtgt tgtctgggca aggcgttaca 2401 gaattatggc ctggtggtcg atggccgtat cgcgctgtgc agcgagaact gtgaagagtt 2461 tttcattccg gtgatcgcgg gtttgtttat tggcgtgggc gttgcaccga ccaatgaaat 2521 ctacacgctg cgcgaactgg tgcactccct gggtatctct aagccgacga tcgttttcag 2581 ctccaagaaa ggtttggata aagttattac ggtgcaaaag acggtcacca cgatcaaaac 2641 gattgtgatc ctggacagca aagttgatta ccgcggttat caatgcctgg atacgttcat 2701 caagcgtaac accccgccag gttttcaagc atctagcttc aaaacggtgg aggttgaccg 2761 taaagaacaa gtcgcactga ttatgaatag ctcgggtagc acgggcctgc caaagggtgt 2821 gcagctgacc catgaaaaca ccgttacccg ttttagccac gcgcgtgatc cgatttacgg 2881 caatcaagtg tcgccgggta cggctgttct gactgttgtc ccttttcacc atggttttgg 2941 catgtttacc actctgggtt acctgatttg cggttttcgc gtcgttatgc tgaccaagtt 3001 tgatgaagaa accttcctga aaaccctgca agactacaag tgcactagtg tgattctggt 3061 cccgacgctg ttcgcgatcc tgaacaaaag cgagctgctg aataagtacg acctgagcaa 3121 tttggtggag attgcgagcg gcggtgctcc gttgagcaaa gaagtaggcg aggcagtggc 3181 acgtcgcttt aacctgcctg gcgtccgtca aggttacggt ttgactgaaa ccacgagcgc 3241 gattatcatc accccggaag gtgacgataa acctggcgct agcggtaagg tcgtgccgtt 3301 gttcaaggct aaagttattg atctggacac caagaagagc ctgggcccga atcgccgtgg 3361 tgaagtttgc gttaagggcc cgatgctgat gaaaggttat gtaaacaatc cggaagcaac 3421 gaaagagctg atcgacgaag agggctggct gcacactggc gatatcggct actacgatga 3481 agagaaacac tttttcattg ttgatcgcct gaaaagcctg atcaagtaca agggttacca 3541 agtcccgcca gcggagctgg aaagcgtact gttgcagcac ccgtcgattt tcgacgccgg 3601 tgtggctggt gtgcctgatc cggtggctgg cgaactgccg ggtgctgtcg tggtgctgga 3661 gagcggtaag aatatgaccg aaaaagaagt catggactat gtggcgagcc aagtgtccaa 3721 tgcaaaacgt ctgcgtggcg gtgttcgctt tgtggacgaa gtgccgaaag gtctgaccgg 3781 caaaattgac ggtcgtgcaa ttcgtgagat tctgaagaaa ccggtcgcga aaatgtaata 3841 a //