LOCUS BBa_K325210 2623 bp DNA linear 25-Apr-2024 DEFINITION Red Firefly Luciferase and LRE
L. Cruciata
(E. coli optimised) ACCESSION BBa_K325210 VERSION BBa_K325210.1 FEATURES Location/Qualifiers RBS 1..17 /label=rbs CDS 18..944 /label=Luciferin regenerating enzyme (L. cruciata) misc_feature 945..950 /label=double stop codon misc_feature 951..956 /label=BamH1 site RBS 957..973 /label=rbs CDS 974..2617 /label=Red luciferase (286) (L. cruciata) misc_feature 2618..2623 /label=double stop codon misc_feature 1830..1830 /label=G to A: Ser286Asn ORIGIN 1 ttaaggaggt aaaaaaaatg gccccgaccg tggaacaaat tgtggagctg ggtacgtatc 61 tgctggccga aagcccgcac tgggacgatg aaacgcaaag cctgtatttc gtcgatatcg 121 ttggtcgcag cgtgaacaaa tacgttccga cgaccaagac gcatacccaa ctgaaattcg 181 acaagaatcc gagcttcatc atcccggtga agggttgttc cgaccgtttt atcgtcagcc 241 tggaacgcga gatcaacctg ctgacctggg acggcgcatc ctctgcacca tccaagattg 301 agaagatcgc ggttttcgat aatacgccag agaagagcga aaaccgtctg aacgatggta 361 aggcagatcc gctgggcaac ctgtgggcgg gtaccatgaa tatgggttct gaccatacca 421 ccggcacgcc ggtgcgcggt accctgagca gcctgagcaa caagcaagtc aaagagcacg 481 tcagcgaggt ctgcatcagc aatggcctgg catggagcaa ggacctgaag aaattctatt 541 acattgatag cgctgttcgt caagttgacc agttcgattt cgatgcgaaa aacctgagct 601 tgagcaatcg tcaaccgctg ttcacctttg acaaacacgg catcatgggt agcccggatg 661 gtcagaccat cgatgcggag ggtaacctgt gggttgccac ctgtcagggc gataaagtcc 721 tgaagattga tacttctacc ccggaaaccc tgctgggtat tgtggagatt ccggaacatc 781 aggtgacgag cgtttgcatc ggcggtgcag aactgaacgt tttgtatgtg acgaccgcgt 841 ccattaaact gcctggtgcg gatgaaacca agccgatgaa aggcgccatc tataaagtca 901 ccggtctggg tgtcaaaggt ctgccgggtg accgtgttaa gctgtaataa ggatccttaa 961 ggaggtaaaa aaaatggaga acatggagaa cgacgagaat attgtggttg gtccgaaacc 1021 gttctacccg atcgaagagg gcagcgcggg tacccagctg cgtaaataca tggaacgcta 1081 cgcgaaactg ggtgcgatcg cgttcacgaa tgcggttacc ggcgtggact attcctatgc 1141 ggagtatttg gaaaagtcgt gttgtctggg caaggcgtta cagaattatg gcctggtggt 1201 cgatggccgt atcgcgctgt gcagcgagaa ctgtgaagag tttttcattc cggtgatcgc 1261 gggtttgttt attggcgtgg gcgttgcacc gaccaatgaa atctacacgc tgcgcgaact 1321 ggtgcactcc ctgggtatct ctaagccgac gatcgttttc agctccaaga aaggtttgga 1381 taaagttatt acggtgcaaa agacggtcac cacgatcaaa acgattgtga tcctggacag 1441 caaagttgat taccgcggtt atcaatgcct ggatacgttc atcaagcgta acaccccgcc 1501 aggttttcaa gcatctagct tcaaaacggt ggaggttgac cgtaaagaac aagtcgcact 1561 gattatgaat agctcgggta gcacgggcct gccaaagggt gtgcagctga cccatgaaaa 1621 caccgttacc cgttttagcc acgcgcgtga tccgatttac ggcaatcaag tgtcgccggg 1681 tacggctgtt ctgactgttg tcccttttca ccatggtttt ggcatgttta ccactctggg 1741 ttacctgatt tgcggttttc gcgtcgttat gctgaccaag tttgatgaag aaaccttcct 1801 gaaaaccctg caagactaca agtgcactaa tgtgattctg gtcccgacgc tgttcgcgat 1861 cctgaacaaa agcgagctgc tgaataagta cgacctgagc aatttggtgg agattgcgag 1921 cggcggtgct ccgttgagca aagaagtagg cgaggcagtg gcacgtcgct ttaacctgcc 1981 tggcgtccgt caaggttacg gtttgactga aaccacgagc gcgattatca tcaccccgga 2041 aggtgacgat aaacctggcg ctagcggtaa ggtcgtgccg ttgttcaagg ctaaagttat 2101 tgatctggac accaagaaga gcctgggccc gaatcgccgt ggtgaagttt gcgttaaggg 2161 cccgatgctg atgaaaggtt atgtaaacaa tccggaagca acgaaagagc tgatcgacga 2221 agagggctgg ctgcacactg gcgatatcgg ctactacgat gaagagaaac actttttcat 2281 tgttgatcgc ctgaaaagcc tgatcaagta caagggttac caagtcccgc cagcggagct 2341 ggaaagcgta ctgttgcagc acccgtcgat tttcgacgcc ggtgtggctg gtgtgcctga 2401 tccggtggct ggcgaactgc cgggtgctgt cgtggtgctg gagagcggta agaatatgac 2461 cgaaaaagaa gtcatggact atgtggcgag ccaagtgtcc aatgcaaaac gtctgcgtgg 2521 cggtgttcgc tttgtggacg aagtgccgaa aggtctgacc ggcaaaattg acggtcgtgc 2581 aattcgtgag attctgaaga aaccggtcgc gaaaatgtaa taa //