LOCUS BBa_K325218 2885 bp DNA linear 17-May-2024 DEFINITION Orange Firefly Luciferase (under pBAD)
L. Cruciata
(E. coli optimised) ACCESSION BBa_K325218 VERSION BBa_K325218.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1219..1235 /label=rbs CDS 1236..2879 /label=Orange luciferase misc_feature 2880..2885 /label=stop misc_feature 2092..2092 /label=G to A: Ser286Asn misc_feature 1219..2885 /label=BBa_K325211 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagtt aaggaggtaa aaaaaatgga gaacatggag aacgacgaga 1261 atattgtggt tggtccgaaa ccgttctacc cgatcgaaga gggcagcgcg ggtacccagc 1321 tgcgtaaata catggaacgc tacgcgaaac tgggtgcgat cgcgttcacg aatgcggtta 1381 ccggcgtgga ctattcctat gcggagtatt tggaaaagtc gtgttgtctg ggcaaggcgt 1441 tacagaatta tggcctggtg gtcgatggcc gtatcgcgct gtgcagcgag aactgtgaag 1501 agtttttcat tccggtgatc gcgggtttgt ttattggcgt gggcgttgca ccgaccaatg 1561 aaatctacac gctgcgcgaa ctggtgcact ccctgggtat ctctaagccg acgatcgttt 1621 tcagctccaa gaaaggtttg gataaagtta ttacggtgca aaagacggtc accacgatca 1681 aaacgattgt gatcctggac agcaaagttg attaccgcgg ttatcaatgc ctggatacgt 1741 tcatcaagcg taacaccccg ccaggttttc aagcatctag cttcaaaacg gtggaggttg 1801 accgtaaaga acaagtcgca ctgattatga atagctcggg tagcacgggc ctgccaaagg 1861 gtgtgcagct gacccatgaa aacaccgtta cccgttttag ccacgcgcgt gatccgattt 1921 acggcaatca agtgtcgccg ggtacggctg ttctgactgt tgtccctttt caccatggtt 1981 ttggcatgtt taccactctg ggttacctga tttgcggttt tcgcgtcgtt atgctgacca 2041 agtttgatga agaaaccttc ctgaaaaccc tgcaagacta caagtgcact aatgtgattc 2101 tggtcccgac gctgttcgcg atcctgaaca aaagcgagct gctgaataag tacgacctga 2161 gcaatttggt ggagattgcg agcggcggtg ctccgttgag caaagaagta ggcgaggcag 2221 tggcacgtcg ctttaacctg cctggcgtcc gtcaaggtta cggtttgact gaaaccacga 2281 gcgcgattat catcaccccg gaaggtgacg ataaacctgg cgctagcggt aaggtcgtgc 2341 cgttgttcaa ggctaaagtt attgatctgg acaccaagaa gagcctgggc ccgaatcgcc 2401 gtggtgaagt ttgcgttaag ggcccgatgc tgatgaaagg ttatgtaaac aatccggaag 2461 caacgaaaga gctgatcgac gaagagggct ggctgcacac tggcgatatc ggctactacg 2521 atgaagagaa acactttttc attgttgatc gcctgaaaag cctgatcaag tacaagggtt 2581 accaagtccc gccagcggag ctggaaagcg tactgttgca gcacccgtcg attttcgacg 2641 ccggtgtggc tggtgtgcct gatccggtgg ctggcgaact gccgggtgct gtcgtggtgc 2701 tggagagcgg taagaatatg accgaaaaag aagtcatgga ctatgtggcg agccaagtgt 2761 ccaatgcaaa acgtctgcgt ggcggtgttc gctttgtgga cgaagtgccg aaaggtctga 2821 ccggcaaaat tgacggtcgt gcaattcgtg agattctgaa gaaaccggtc gcgaaaatgt 2881 aataa //