LOCUS       BBa_K325219             3841 bp    DNA     linear       17-May-2024
DEFINITION  Red Firefly Luciferase and LRE (under pBAD)<BR><i>L. 
            Cruciata<BR>(E. coli optimised)</i>
ACCESSION   BBa_K325219
VERSION     BBa_K325219.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1235
                     /label=rbs
     CDS             1236..2162
                     /label=Luciferin regenerating enzyme (L. cruciata)
     misc_feature    2163..2168
                     /label=double stop codon
     misc_feature    2169..2174
                     /label=BamH1 site
     RBS             2175..2191
                     /label=rbs
     CDS             2192..3835
                     /label=Red luciferase (286) (L. cruciata)
     misc_feature    3836..3841
                     /label=double stop codon
     misc_feature    3048..3048
                     /label=G to A: Ser286Asn
     misc_feature    1219..3841
                     /label=BBa_K325210
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtt aaggaggtaa aaaaaatggc cccgaccgtg gaacaaattg
     1261 tggagctggg tacgtatctg ctggccgaaa gcccgcactg ggacgatgaa acgcaaagcc
     1321 tgtatttcgt cgatatcgtt ggtcgcagcg tgaacaaata cgttccgacg accaagacgc
     1381 atacccaact gaaattcgac aagaatccga gcttcatcat cccggtgaag ggttgttccg
     1441 accgttttat cgtcagcctg gaacgcgaga tcaacctgct gacctgggac ggcgcatcct
     1501 ctgcaccatc caagattgag aagatcgcgg ttttcgataa tacgccagag aagagcgaaa
     1561 accgtctgaa cgatggtaag gcagatccgc tgggcaacct gtgggcgggt accatgaata
     1621 tgggttctga ccataccacc ggcacgccgg tgcgcggtac cctgagcagc ctgagcaaca
     1681 agcaagtcaa agagcacgtc agcgaggtct gcatcagcaa tggcctggca tggagcaagg
     1741 acctgaagaa attctattac attgatagcg ctgttcgtca agttgaccag ttcgatttcg
     1801 atgcgaaaaa cctgagcttg agcaatcgtc aaccgctgtt cacctttgac aaacacggca
     1861 tcatgggtag cccggatggt cagaccatcg atgcggaggg taacctgtgg gttgccacct
     1921 gtcagggcga taaagtcctg aagattgata cttctacccc ggaaaccctg ctgggtattg
     1981 tggagattcc ggaacatcag gtgacgagcg tttgcatcgg cggtgcagaa ctgaacgttt
     2041 tgtatgtgac gaccgcgtcc attaaactgc ctggtgcgga tgaaaccaag ccgatgaaag
     2101 gcgccatcta taaagtcacc ggtctgggtg tcaaaggtct gccgggtgac cgtgttaagc
     2161 tgtaataagg atccttaagg aggtaaaaaa aatggagaac atggagaacg acgagaatat
     2221 tgtggttggt ccgaaaccgt tctacccgat cgaagagggc agcgcgggta cccagctgcg
     2281 taaatacatg gaacgctacg cgaaactggg tgcgatcgcg ttcacgaatg cggttaccgg
     2341 cgtggactat tcctatgcgg agtatttgga aaagtcgtgt tgtctgggca aggcgttaca
     2401 gaattatggc ctggtggtcg atggccgtat cgcgctgtgc agcgagaact gtgaagagtt
     2461 tttcattccg gtgatcgcgg gtttgtttat tggcgtgggc gttgcaccga ccaatgaaat
     2521 ctacacgctg cgcgaactgg tgcactccct gggtatctct aagccgacga tcgttttcag
     2581 ctccaagaaa ggtttggata aagttattac ggtgcaaaag acggtcacca cgatcaaaac
     2641 gattgtgatc ctggacagca aagttgatta ccgcggttat caatgcctgg atacgttcat
     2701 caagcgtaac accccgccag gttttcaagc atctagcttc aaaacggtgg aggttgaccg
     2761 taaagaacaa gtcgcactga ttatgaatag ctcgggtagc acgggcctgc caaagggtgt
     2821 gcagctgacc catgaaaaca ccgttacccg ttttagccac gcgcgtgatc cgatttacgg
     2881 caatcaagtg tcgccgggta cggctgttct gactgttgtc ccttttcacc atggttttgg
     2941 catgtttacc actctgggtt acctgatttg cggttttcgc gtcgttatgc tgaccaagtt
     3001 tgatgaagaa accttcctga aaaccctgca agactacaag tgcactaatg tgattctggt
     3061 cccgacgctg ttcgcgatcc tgaacaaaag cgagctgctg aataagtacg acctgagcaa
     3121 tttggtggag attgcgagcg gcggtgctcc gttgagcaaa gaagtaggcg aggcagtggc
     3181 acgtcgcttt aacctgcctg gcgtccgtca aggttacggt ttgactgaaa ccacgagcgc
     3241 gattatcatc accccggaag gtgacgataa acctggcgct agcggtaagg tcgtgccgtt
     3301 gttcaaggct aaagttattg atctggacac caagaagagc ctgggcccga atcgccgtgg
     3361 tgaagtttgc gttaagggcc cgatgctgat gaaaggttat gtaaacaatc cggaagcaac
     3421 gaaagagctg atcgacgaag agggctggct gcacactggc gatatcggct actacgatga
     3481 agagaaacac tttttcattg ttgatcgcct gaaaagcctg atcaagtaca agggttacca
     3541 agtcccgcca gcggagctgg aaagcgtact gttgcagcac ccgtcgattt tcgacgccgg
     3601 tgtggctggt gtgcctgatc cggtggctgg cgaactgccg ggtgctgtcg tggtgctgga
     3661 gagcggtaag aatatgaccg aaaaagaagt catggactat gtggcgagcc aagtgtccaa
     3721 tgcaaaacgt ctgcgtggcg gtgttcgctt tgtggacgaa gtgccgaaag gtctgaccgg
     3781 caaaattgac ggtcgtgcaa ttcgtgagat tctgaagaaa ccggtcgcga aaatgtaata
     3841 a
//