##gff-version 3
##sequence-region BBa_K325219 1 3841
BBa_K325219	.	engineered_region	1219	3841	.	+	0	ID=BBa_K325210;Name=BBa_K325210
BBa_K325219	.	sequence_alteration	3048	3048	.	+	0	ID=annotation2103099;Name=G to A: Ser286Asn;Parent=BBa_K325210
BBa_K325219	.	CDS	1236	2162	.	+	0	ID=annotation2080150;Name=Luciferin regenerating enzyme (L. cruciata);Parent=BBa_K325210
BBa_K325219	.	non_covalent_binding_site	2169	2174	.	+	0	ID=annotation2080152;Name=BamH1 site;Parent=BBa_K325210
BBa_K325219	.	CDS	2192	3835	.	+	0	ID=annotation2080154;Name=Red luciferase (286) (L. cruciata);Parent=BBa_K325210
BBa_K325219	.	ribosome_entry_site	1219	1235	.	+	0	ID=annotation2080149;Name=rbs;Parent=BBa_K325210
BBa_K325219	.	stop_codon	2163	2168	.	+	0	ID=annotation2080151;Name=double stop codon;Parent=BBa_K325210
BBa_K325219	.	stop_codon	3836	3841	.	+	0	ID=annotation2080155;Name=double stop codon;Parent=BBa_K325210
BBa_K325219	.	ribosome_entry_site	2175	2191	.	+	0	ID=annotation2080153;Name=rbs;Parent=BBa_K325210
BBa_K325219	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500
BBa_K325219	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_K325219	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_K325219	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
>BBa_K325219
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagttaaggaggtaaaaaaaatggccccgaccgtggaacaaattgtgga
gctgggtacgtatctgctggccgaaagcccgcactgggacgatgaaacgcaaagcctgtatttcgtcgatatcgttggt
cgcagcgtgaacaaatacgttccgacgaccaagacgcatacccaactgaaattcgacaagaatccgagcttcatcatcc
cggtgaagggttgttccgaccgttttatcgtcagcctggaacgcgagatcaacctgctgacctgggacggcgcatcctc
tgcaccatccaagattgagaagatcgcggttttcgataatacgccagagaagagcgaaaaccgtctgaacgatggtaag
gcagatccgctgggcaacctgtgggcgggtaccatgaatatgggttctgaccataccaccggcacgccggtgcgcggta
ccctgagcagcctgagcaacaagcaagtcaaagagcacgtcagcgaggtctgcatcagcaatggcctggcatggagcaa
ggacctgaagaaattctattacattgatagcgctgttcgtcaagttgaccagttcgatttcgatgcgaaaaacctgagc
ttgagcaatcgtcaaccgctgttcacctttgacaaacacggcatcatgggtagcccggatggtcagaccatcgatgcgg
agggtaacctgtgggttgccacctgtcagggcgataaagtcctgaagattgatacttctaccccggaaaccctgctggg
tattgtggagattccggaacatcaggtgacgagcgtttgcatcggcggtgcagaactgaacgttttgtatgtgacgacc
gcgtccattaaactgcctggtgcggatgaaaccaagccgatgaaaggcgccatctataaagtcaccggtctgggtgtca
aaggtctgccgggtgaccgtgttaagctgtaataaggatccttaaggaggtaaaaaaaatggagaacatggagaacgac
gagaatattgtggttggtccgaaaccgttctacccgatcgaagagggcagcgcgggtacccagctgcgtaaatacatgg
aacgctacgcgaaactgggtgcgatcgcgttcacgaatgcggttaccggcgtggactattcctatgcggagtatttgga
aaagtcgtgttgtctgggcaaggcgttacagaattatggcctggtggtcgatggccgtatcgcgctgtgcagcgagaac
tgtgaagagtttttcattccggtgatcgcgggtttgtttattggcgtgggcgttgcaccgaccaatgaaatctacacgc
tgcgcgaactggtgcactccctgggtatctctaagccgacgatcgttttcagctccaagaaaggtttggataaagttat
tacggtgcaaaagacggtcaccacgatcaaaacgattgtgatcctggacagcaaagttgattaccgcggttatcaatgc
ctggatacgttcatcaagcgtaacaccccgccaggttttcaagcatctagcttcaaaacggtggaggttgaccgtaaag
aacaagtcgcactgattatgaatagctcgggtagcacgggcctgccaaagggtgtgcagctgacccatgaaaacaccgt
tacccgttttagccacgcgcgtgatccgatttacggcaatcaagtgtcgccgggtacggctgttctgactgttgtccct
tttcaccatggttttggcatgtttaccactctgggttacctgatttgcggttttcgcgtcgttatgctgaccaagtttg
atgaagaaaccttcctgaaaaccctgcaagactacaagtgcactaatgtgattctggtcccgacgctgttcgcgatcct
gaacaaaagcgagctgctgaataagtacgacctgagcaatttggtggagattgcgagcggcggtgctccgttgagcaaa
gaagtaggcgaggcagtggcacgtcgctttaacctgcctggcgtccgtcaaggttacggtttgactgaaaccacgagcg
cgattatcatcaccccggaaggtgacgataaacctggcgctagcggtaaggtcgtgccgttgttcaaggctaaagttat
tgatctggacaccaagaagagcctgggcccgaatcgccgtggtgaagtttgcgttaagggcccgatgctgatgaaaggt
tatgtaaacaatccggaagcaacgaaagagctgatcgacgaagagggctggctgcacactggcgatatcggctactacg
atgaagagaaacactttttcattgttgatcgcctgaaaagcctgatcaagtacaagggttaccaagtcccgccagcgga
gctggaaagcgtactgttgcagcacccgtcgattttcgacgccggtgtggctggtgtgcctgatccggtggctggcgaa
ctgccgggtgctgtcgtggtgctggagagcggtaagaatatgaccgaaaaagaagtcatggactatgtggcgagccaag
tgtccaatgcaaaacgtctgcgtggcggtgttcgctttgtggacgaagtgccgaaaggtctgaccggcaaaattgacgg
tcgtgcaattcgtgagattctgaagaaaccggtcgcgaaaatgtaataa