LOCUS BBa_K325905 6478 bp DNA linear 22-Dec-2024 DEFINITION Bacterial Lux reporter
CDEG pλ AB
(E. coli optimised) ACCESSION BBa_K325905 VERSION BBa_K325905.1 FEATURES Location/Qualifiers RBS 1..17 /label=rbs CDS 18..1457 /label=LuxC RBS 1458..1475 /label=rbs CDS 1476..2398 /label=LuxD CDS 2416..3552 /label=LuxE RBS 3553..3569 /label=rbs RBS 2399..2415 /label=rbs CDS 3570..4280 /label=LuxG stem_loop 4281..4319 /label=terminator promoter 4320..4368 /label=pLambda RBS 4383..4388 /label=rbs CDS 4395..5477 /label=LuxA RBS 5480..5485 /label=rbs CDS 5492..6475 /label=LuxB ORIGIN 1 ttaaggaggt aaaaaaaatg aacaaatgca tcccgatgat catcaacggt atgatccagg 61 acttcgacaa ctacgcttac aaggaggtaa agctgaacaa cgacaaccgt gttaagctgt 121 ccgttatcac cgagtcttct gtttctaaaa ccctgaacat caaagaccgt atcaacctga 181 acctgaacca gatcgttaac ttcctgtaca ccgttggtca gcgttggaaa tctgaagaat 241 acaaccgtcg tcgtacctac atccgtgaac tgaaaaccta cctgggttac tctgacgaaa 301 tggcgcgtct ggaagcgaac tggatcgcga tgctgctgtg ctctaaatct gcgctgtacg 361 acatcgttaa ctacgacctg ggttctatcc acgttctgga cgaatggctg ccgcgtggtg 421 actgctacgt taaagcgcag ccgaaaggtg tttctgttca cctgctggcg ggtaacgttc 481 cgctgtctgg tgttacctct atcctgcgtg cgatcctgac caaaaacgaa tgcatcatca 541 aaacctcttc ttctgacccg ttcaccgcga acgcgctggt ttcttctttc atcgacgtta 601 acgcggacca cccgatcacc aaatctatgt ctgttatgta ctggccgcac gacgaagaca 661 tgaccctgtc tcagcgtatc atgaaccacg cggacgttgt tatcgcgtgg ggtggtgacg 721 aagcgatcaa atgggcggtt aaatactctc cgccgcacgt tgacatcctg aaattcggtc 781 cgaaaaaatc tctgtctatc atcgaagcgc cgaaagacat cgaagcggcg gcgatgggtg 841 ttgcgcacga catctgcttc tacgaccagc aggcgtgctt ctctacccag gacgtttact 901 acatcggtga caacctgccg ctgttcctga acgaactgga aaaacagctg gaccgttacg 961 cgaaaatcct gccgaaaggt tctaactctt tcgacgaaaa agcggcgttc accctgaccg 1021 aaaaagaatc tctgttcgcg ggttacgaag ttcgtaaagg tgacaaacag gcgtggctga 1081 tcgttgtttc tccgaccaac tctttcggta accagccgct gtctcgttct gtttacgttc 1141 accaggtttc tgacatcaaa gaaatcatcc cgttcgttaa caaaaaccgt acccagaccg 1201 tttctatcta cccgtgggaa gcgtctctga aataccgtga caaactggcg cgttctggtg 1261 ttgaacgtat cgttgaatct ggtatgaaca acatcttccg tgttggtggt gcgcacgact 1321 ctctgtctcc gctgcaatac ctggttcgtt tcgtttctca cgaacgtccg ttcaactaca 1381 ccaccaaaga cgttgcggtt gaaatcgaac agacccgtta cctggaagaa gacaaattcc 1441 tggttttcgt tccgtaatta aggaggtaaa aaaaatgaaa gacgaatctg cgttcttcac 1501 catcgaccac atcatcaaac tggacaacgg tcagtctatc cgtgtttggg aaaccctgcc 1561 gaaaaaaaac gttccggaaa aaaaacacac catcctgatc gcgtctggtt tcgcgcgtcg 1621 tatggaccac ttcgcgggtc tggcggaata cctgtctacc aacggtttcc acgttatccg 1681 ttacgactct ctgcaccacg ttggtctgtc ttctggttgc atcaacgaat ttaccatgtc 1741 tatcggtaaa aactctctgc tgaccgttgt tgactggctg accgaccacg gtgttgaacg 1801 tatcggtctg atcgcggcgt ctctgtctgc gcgtatcgcg tacgaagttg ttaacaaaat 1861 caaactgtct ttcctgatca ccgcggttgg tgttgttaac ctgcgtgaca ccctggaaaa 1921 agcgctggaa tacgactacc tgcaactgcc gatctctgaa ctgccggaag acctggactt 1981 cgaaggtcac aacctgggtt ctgaagtttt cgttaccgac tgcttcaaac acaactggga 2041 caccctggac tctaccctga actctgttaa aggtctggcg atcccgttca tcgcgttcac 2101 cgcgaacgac gactcttggg ttaaacagtc tgaagttatc gaactgatcg actctatcga 2161 atcttctaac tgcaaactgt actctctgat cggttcttct cacgacctgg gtgaaaacct 2221 ggttgttctg cgtaacttct accagtctgt taccaaagcg gcgctggcgc tggacgacgg 2281 tctgctggac ctggaaatcg acatcatcga accgcgtttc gaagacgtta cctctatcac 2341 cgttaaagaa cgtcgtctga aaaacgaaat cgaaaacgaa ctgctggaac tggcgtaatt 2401 aaggaggtaa aaaaaatgac cgttcacacc gaatacaaac gtaaccagat catcgcgtct 2461 tctgaaatcg acgacctgat cttcatgacc aaaccgcagg aatggtcttt cgaagaacag 2521 aaagaaatcc gtgacaaact ggttcgtgaa gcgttctact tccactacaa ccgtaacgaa 2581 gaataccgta actactgcat caaccagcac gtttctgaca acctgcacac catcgacgaa 2641 atcccggttt tcccgacctc tgttttcaaa tacaaaaaac tgcacaccgt taccgcggaa 2701 gacatcgaaa actggtacac ctcttctggt acccgtggtg ttaaatctca catcgcgcgt 2761 gaccgtctgt ctatcgaacg tctgctgggt tctgttaact tcggtatgaa atacgttggt 2821 gactggttcg aacaccagat ggaactgatc aacctgggtc cggaccgttt caacaccaac 2881 aacatctggt tcaaatacgt tatgtctctg gttgaactgc tgtacccgac cgaatttacc 2941 gttgacaacg acaaaatcga cttcgagaaa acagtaaaac acctgttccg tatcaaaaac 3001 tccaaaaaag acatctgcct gatcggtccg ccgttcttcg tttacctgct gtgccagtac 3061 atgaaagaaa acaacatcga atttaaaggt ggtgaccgtg ttcacatcat caccggtggt 3121 ggttggaaat ctaaccagaa cgactctctg gaccgtgcgg acttcaacca gctgctgatg 3181 gacaccttcc agctggacaa aatcaaccag atccgtgaca ccttcaacca ggttgaactg 3241 aacacctgct tcttcgaaga cgaatttcag cgtaaacacg ttccgccgtg ggtttacgcg 3301 cgtgcgctgg acccggaaac cctgaaaccg gttgcggacg gtgaaatcgg tctgctgtct 3361 tacatggacg cgtcttctac cgcgtacccg gcgttcatcg ttaccgacga catcggtatc 3421 gttaaagaaa tccgtgaacc ggacccgtac ccgggtgtta ccgttgaaat cgttcgtcgt 3481 ctgaacaccc gtgcgcagaa aggttgcgcg ctgtctatgg cgaacgttat ccagaaaaac 3541 atcaaagact aattaaggag gtaaaaaaaa tgatcgttga cggtcgtgtt tctaaaatcg 3601 ttctggcgtc tatccgtaac aacatctaca aagttttcat caccgttaac tctccgatca 3661 aattcatcgc gggtcagttc gttatggtta ccatcaacgg taaaaaatgc ccgttctcta 3721 tcgcgaactg cccgaccaaa aactacgaaa tcgaactgca catcggttct tctaaccgtg 3781 actgctctct ggacatcatc gaatacttcg ttgacgctct ggttgaagaa gttgctatcg 3841 aactggacgc tccgcacggt aacgcttggc tgcgttctga atctaacaac ccgctgctgc 3901 tgatcgcggg tggtaccggt ctgtcttaca tcaactctat cctgaccaac tgcctgaacc 3961 gtaacatccc gcaggacatc tacctgtact ggggtgttaa agactcttct ctgctgtacg 4021 aagacgaaga actgctgaac ctgtctctga acaacaaaaa cttccactac atcccggtta 4081 tcgaagacaa atctgaagaa tggatcggtc gtaaaggtac cgttctggac gcggttatgg 4141 aagacttcac cgacctgacc tacttcgaca tctacgtttg cggtccgttc atgatggcga 4201 aaaccgcgaa agaaaaactg atcgaagaaa aaaaagcgaa atctgaacag atgttcgcgg 4261 acgcgttcgc gtacgtttaa aaaaaaaaac cccgcccctg acagggcggg gttttttttt 4321 aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcct caaatagcaa 4381 tataaggact ctctatgaaa tttggaaact ttttgcttac ataccaacct ccccaatttt 4441 ctcaaacaga ggtaatgaaa cgtttggtta aattaggtcg catctctgag gagtgtggtt 4501 ttgataccgt atggttactg gagcatcatt tcacggagtt tggtttgctt ggtaaccctt 4561 atgtcgctgc tgcatattta cttggcgcga ctaaaaaatt gaatgtagga actgccgcta 4621 ttgttcttcc cacagcccat ccagtacgcc aacttgaaga tgtgaattta ttggatcaaa 4681 tgtcaaaagg acgatttcgg tttggtattt gccgagggct ttacaacaag gactttcgcg 4741 tattcggcac agatatgaat aacagtcgcg ccttagcgga atgctggtac gggctgataa 4801 agaatggcat gacagaggga tatatggaag ctgataatga acatatcaag ttccataagg 4861 taaaagtaaa ccccgcggcg tatagcagag gtggcgcacc ggtttatgtg gtggctgaat 4921 cagcttcgac gactgagtgg gctgctcaat ttggcctacc gatgatatta agttggatta 4981 taaatactaa cgaaaagaaa gcacaacttg agctttataa tgaagtggct caagaatatg 5041 ggcacgatat tcataatatc gaccattgct tatcatatat aacatctgta gatcatgact 5101 caattaaagc gaaagagatt tgccggaaat ttctggggca ttggtatgat tcttatgtga 5161 atgctacgac tatttttgat gattcagacc aaacaagagg ttatgatttc aataaagggc 5221 agtggcgtga ctttgtatta aaaggacata aagatactaa tcgccgtatt gattacagtt 5281 acgaaatcaa tcccgtggga acgccgcagg aatgtattga cataattcaa aaagacattg 5341 atgctacagg aatatcaaat atttgttgtg gatttgaagc taatggaaca gtagacgaaa 5401 ttattgcttc catgaagctc ttccagtctg atgtcatgcc atttcttaaa gaaaaacaac 5461 gttcgctatt atattagcta aggagaaaga aatgaaattt ggattgttct tccttaactt 5521 catcaattca acaactgttc aagaacaaag tatagttcgc atgcaggaaa taacggagta 5581 tgttgataag ttgaattttg aacagatttt agtgtatgaa aatcattttt cagataatgg 5641 tgttgtcggc gctcctctga ctgtttctgg ttttctgctc ggtttaacag agaaaattaa 5701 aattggttca ttaaatcaca tcattacaac tcatcatcct gtcgccatag cggaggaagc 5761 ttgcttattg gatcagttaa gtgaagggag atttatttta gggtttagtg attgcgaaaa 5821 aaaagatgaa atgcattttt ttaatcgccc ggttgaatat caacagcaac tatttgaaga 5881 gtgttatgaa atcattaacg atgctttaac aacaggctat tgtaatccag ataacgattt 5941 ttatagcttc cctaaaatat ctgtaaatcc ccatgcttat acgccaggcg gacctcggaa 6001 atatgtaaca gcaaccagtc atcatattgt tgagtgggcg gccaaaaaag gtattcctct 6061 catctttaag tgggatgatt ctaatgatgt tagatatgaa tatgctgaaa gatataaagc 6121 cgttgcggat aaatatgacg ttgacctatc agagatagac catcagttaa tgatattagt 6181 taactataac gaagatagta ataaagctaa acaagagacg cgtgcattta ttagtgatta 6241 tgttcttgaa atgcacccta atgaaaattt cgaaaataaa cttgaagaaa taattgcaga 6301 aaacgctgtc ggaaattata cggagtgtat aactgcggct aagttggcaa ttgaaaagtg 6361 tggtgcgaaa agtgtattgc tgtcctttga accaatgaat gatttgatga gccaaaaaaa 6421 tgtaatcaat attgttgatg ataatattaa gaagtaccac atggaatata cctaataa //