LOCUS BBa_K325906 7688 bp DNA linear 20-Nov-2024 DEFINITION pbad CDEG plambda AB ACCESSION BBa_K325906 VERSION BBa_K325906.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 RBS 1211..1227 /label=rbs CDS 1228..2667 /label=LuxC RBS 2668..2685 /label=rbs CDS 2686..3608 /label=LuxD CDS 3626..4762 /label=LuxE RBS 4763..4779 /label=rbs RBS 3609..3625 /label=rbs CDS 4780..5490 /label=LuxG stem_loop 5491..5529 /label=terminator promoter 5530..5578 /label=pLambda RBS 5593..5598 /label=rbs CDS 5605..6687 /label=LuxA RBS 6690..6695 /label=rbs CDS 6702..7685 /label=LuxB misc_feature 1211..7688 /label=BBa_K325905 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc ttaaggaggt aaaaaaaatg aacaaatgca tcccgatgat catcaacggt 1261 atgatccagg acttcgacaa ctacgcttac aaggaggtaa agctgaacaa cgacaaccgt 1321 gttaagctgt ccgttatcac cgagtcttct gtttctaaaa ccctgaacat caaagaccgt 1381 atcaacctga acctgaacca gatcgttaac ttcctgtaca ccgttggtca gcgttggaaa 1441 tctgaagaat acaaccgtcg tcgtacctac atccgtgaac tgaaaaccta cctgggttac 1501 tctgacgaaa tggcgcgtct ggaagcgaac tggatcgcga tgctgctgtg ctctaaatct 1561 gcgctgtacg acatcgttaa ctacgacctg ggttctatcc acgttctgga cgaatggctg 1621 ccgcgtggtg actgctacgt taaagcgcag ccgaaaggtg tttctgttca cctgctggcg 1681 ggtaacgttc cgctgtctgg tgttacctct atcctgcgtg cgatcctgac caaaaacgaa 1741 tgcatcatca aaacctcttc ttctgacccg ttcaccgcga acgcgctggt ttcttctttc 1801 atcgacgtta acgcggacca cccgatcacc aaatctatgt ctgttatgta ctggccgcac 1861 gacgaagaca tgaccctgtc tcagcgtatc atgaaccacg cggacgttgt tatcgcgtgg 1921 ggtggtgacg aagcgatcaa atgggcggtt aaatactctc cgccgcacgt tgacatcctg 1981 aaattcggtc cgaaaaaatc tctgtctatc atcgaagcgc cgaaagacat cgaagcggcg 2041 gcgatgggtg ttgcgcacga catctgcttc tacgaccagc aggcgtgctt ctctacccag 2101 gacgtttact acatcggtga caacctgccg ctgttcctga acgaactgga aaaacagctg 2161 gaccgttacg cgaaaatcct gccgaaaggt tctaactctt tcgacgaaaa agcggcgttc 2221 accctgaccg aaaaagaatc tctgttcgcg ggttacgaag ttcgtaaagg tgacaaacag 2281 gcgtggctga tcgttgtttc tccgaccaac tctttcggta accagccgct gtctcgttct 2341 gtttacgttc accaggtttc tgacatcaaa gaaatcatcc cgttcgttaa caaaaaccgt 2401 acccagaccg tttctatcta cccgtgggaa gcgtctctga aataccgtga caaactggcg 2461 cgttctggtg ttgaacgtat cgttgaatct ggtatgaaca acatcttccg tgttggtggt 2521 gcgcacgact ctctgtctcc gctgcaatac ctggttcgtt tcgtttctca cgaacgtccg 2581 ttcaactaca ccaccaaaga cgttgcggtt gaaatcgaac agacccgtta cctggaagaa 2641 gacaaattcc tggttttcgt tccgtaatta aggaggtaaa aaaaatgaaa gacgaatctg 2701 cgttcttcac catcgaccac atcatcaaac tggacaacgg tcagtctatc cgtgtttggg 2761 aaaccctgcc gaaaaaaaac gttccggaaa aaaaacacac catcctgatc gcgtctggtt 2821 tcgcgcgtcg tatggaccac ttcgcgggtc tggcggaata cctgtctacc aacggtttcc 2881 acgttatccg ttacgactct ctgcaccacg ttggtctgtc ttctggttgc atcaacgaat 2941 ttaccatgtc tatcggtaaa aactctctgc tgaccgttgt tgactggctg accgaccacg 3001 gtgttgaacg tatcggtctg atcgcggcgt ctctgtctgc gcgtatcgcg tacgaagttg 3061 ttaacaaaat caaactgtct ttcctgatca ccgcggttgg tgttgttaac ctgcgtgaca 3121 ccctggaaaa agcgctggaa tacgactacc tgcaactgcc gatctctgaa ctgccggaag 3181 acctggactt cgaaggtcac aacctgggtt ctgaagtttt cgttaccgac tgcttcaaac 3241 acaactggga caccctggac tctaccctga actctgttaa aggtctggcg atcccgttca 3301 tcgcgttcac cgcgaacgac gactcttggg ttaaacagtc tgaagttatc gaactgatcg 3361 actctatcga atcttctaac tgcaaactgt actctctgat cggttcttct cacgacctgg 3421 gtgaaaacct ggttgttctg cgtaacttct accagtctgt taccaaagcg gcgctggcgc 3481 tggacgacgg tctgctggac ctggaaatcg acatcatcga accgcgtttc gaagacgtta 3541 cctctatcac cgttaaagaa cgtcgtctga aaaacgaaat cgaaaacgaa ctgctggaac 3601 tggcgtaatt aaggaggtaa aaaaaatgac cgttcacacc gaatacaaac gtaaccagat 3661 catcgcgtct tctgaaatcg acgacctgat cttcatgacc aaaccgcagg aatggtcttt 3721 cgaagaacag aaagaaatcc gtgacaaact ggttcgtgaa gcgttctact tccactacaa 3781 ccgtaacgaa gaataccgta actactgcat caaccagcac gtttctgaca acctgcacac 3841 catcgacgaa atcccggttt tcccgacctc tgttttcaaa tacaaaaaac tgcacaccgt 3901 taccgcggaa gacatcgaaa actggtacac ctcttctggt acccgtggtg ttaaatctca 3961 catcgcgcgt gaccgtctgt ctatcgaacg tctgctgggt tctgttaact tcggtatgaa 4021 atacgttggt gactggttcg aacaccagat ggaactgatc aacctgggtc cggaccgttt 4081 caacaccaac aacatctggt tcaaatacgt tatgtctctg gttgaactgc tgtacccgac 4141 cgaatttacc gttgacaacg acaaaatcga cttcgagaaa acagtaaaac acctgttccg 4201 tatcaaaaac tccaaaaaag acatctgcct gatcggtccg ccgttcttcg tttacctgct 4261 gtgccagtac atgaaagaaa acaacatcga atttaaaggt ggtgaccgtg ttcacatcat 4321 caccggtggt ggttggaaat ctaaccagaa cgactctctg gaccgtgcgg acttcaacca 4381 gctgctgatg gacaccttcc agctggacaa aatcaaccag atccgtgaca ccttcaacca 4441 ggttgaactg aacacctgct tcttcgaaga cgaatttcag cgtaaacacg ttccgccgtg 4501 ggtttacgcg cgtgcgctgg acccggaaac cctgaaaccg gttgcggacg gtgaaatcgg 4561 tctgctgtct tacatggacg cgtcttctac cgcgtacccg gcgttcatcg ttaccgacga 4621 catcggtatc gttaaagaaa tccgtgaacc ggacccgtac ccgggtgtta ccgttgaaat 4681 cgttcgtcgt ctgaacaccc gtgcgcagaa aggttgcgcg ctgtctatgg cgaacgttat 4741 ccagaaaaac atcaaagact aattaaggag gtaaaaaaaa tgatcgttga cggtcgtgtt 4801 tctaaaatcg ttctggcgtc tatccgtaac aacatctaca aagttttcat caccgttaac 4861 tctccgatca aattcatcgc gggtcagttc gttatggtta ccatcaacgg taaaaaatgc 4921 ccgttctcta tcgcgaactg cccgaccaaa aactacgaaa tcgaactgca catcggttct 4981 tctaaccgtg actgctctct ggacatcatc gaatacttcg ttgacgctct ggttgaagaa 5041 gttgctatcg aactggacgc tccgcacggt aacgcttggc tgcgttctga atctaacaac 5101 ccgctgctgc tgatcgcggg tggtaccggt ctgtcttaca tcaactctat cctgaccaac 5161 tgcctgaacc gtaacatccc gcaggacatc tacctgtact ggggtgttaa agactcttct 5221 ctgctgtacg aagacgaaga actgctgaac ctgtctctga acaacaaaaa cttccactac 5281 atcccggtta tcgaagacaa atctgaagaa tggatcggtc gtaaaggtac cgttctggac 5341 gcggttatgg aagacttcac cgacctgacc tacttcgaca tctacgtttg cggtccgttc 5401 atgatggcga aaaccgcgaa agaaaaactg atcgaagaaa aaaaagcgaa atctgaacag 5461 atgttcgcgg acgcgttcgc gtacgtttaa aaaaaaaaac cccgcccctg acagggcggg 5521 gttttttttt aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgcct 5581 caaatagcaa tataaggact ctctatgaaa tttggaaact ttttgcttac ataccaacct 5641 ccccaatttt ctcaaacaga ggtaatgaaa cgtttggtta aattaggtcg catctctgag 5701 gagtgtggtt ttgataccgt atggttactg gagcatcatt tcacggagtt tggtttgctt 5761 ggtaaccctt atgtcgctgc tgcatattta cttggcgcga ctaaaaaatt gaatgtagga 5821 actgccgcta ttgttcttcc cacagcccat ccagtacgcc aacttgaaga tgtgaattta 5881 ttggatcaaa tgtcaaaagg acgatttcgg tttggtattt gccgagggct ttacaacaag 5941 gactttcgcg tattcggcac agatatgaat aacagtcgcg ccttagcgga atgctggtac 6001 gggctgataa agaatggcat gacagaggga tatatggaag ctgataatga acatatcaag 6061 ttccataagg taaaagtaaa ccccgcggcg tatagcagag gtggcgcacc ggtttatgtg 6121 gtggctgaat cagcttcgac gactgagtgg gctgctcaat ttggcctacc gatgatatta 6181 agttggatta taaatactaa cgaaaagaaa gcacaacttg agctttataa tgaagtggct 6241 caagaatatg ggcacgatat tcataatatc gaccattgct tatcatatat aacatctgta 6301 gatcatgact caattaaagc gaaagagatt tgccggaaat ttctggggca ttggtatgat 6361 tcttatgtga atgctacgac tatttttgat gattcagacc aaacaagagg ttatgatttc 6421 aataaagggc agtggcgtga ctttgtatta aaaggacata aagatactaa tcgccgtatt 6481 gattacagtt acgaaatcaa tcccgtggga acgccgcagg aatgtattga cataattcaa 6541 aaagacattg atgctacagg aatatcaaat atttgttgtg gatttgaagc taatggaaca 6601 gtagacgaaa ttattgcttc catgaagctc ttccagtctg atgtcatgcc atttcttaaa 6661 gaaaaacaac gttcgctatt atattagcta aggagaaaga aatgaaattt ggattgttct 6721 tccttaactt catcaattca acaactgttc aagaacaaag tatagttcgc atgcaggaaa 6781 taacggagta tgttgataag ttgaattttg aacagatttt agtgtatgaa aatcattttt 6841 cagataatgg tgttgtcggc gctcctctga ctgtttctgg ttttctgctc ggtttaacag 6901 agaaaattaa aattggttca ttaaatcaca tcattacaac tcatcatcct gtcgccatag 6961 cggaggaagc ttgcttattg gatcagttaa gtgaagggag atttatttta gggtttagtg 7021 attgcgaaaa aaaagatgaa atgcattttt ttaatcgccc ggttgaatat caacagcaac 7081 tatttgaaga gtgttatgaa atcattaacg atgctttaac aacaggctat tgtaatccag 7141 ataacgattt ttatagcttc cctaaaatat ctgtaaatcc ccatgcttat acgccaggcg 7201 gacctcggaa atatgtaaca gcaaccagtc atcatattgt tgagtgggcg gccaaaaaag 7261 gtattcctct catctttaag tgggatgatt ctaatgatgt tagatatgaa tatgctgaaa 7321 gatataaagc cgttgcggat aaatatgacg ttgacctatc agagatagac catcagttaa 7381 tgatattagt taactataac gaagatagta ataaagctaa acaagagacg cgtgcattta 7441 ttagtgatta tgttcttgaa atgcacccta atgaaaattt cgaaaataaa cttgaagaaa 7501 taattgcaga aaacgctgtc ggaaattata cggagtgtat aactgcggct aagttggcaa 7561 ttgaaaagtg tggtgcgaaa agtgtattgc tgtcctttga accaatgaat gatttgatga 7621 gccaaaaaaa tgtaatcaat attgttgatg ataatattaa gaagtaccac atggaatata 7681 cctaataa //