LOCUS       BBa_K327002             1832 bp    DNA     linear       22-Dec-2024
DEFINITION  Polycistronic Delta, Tet, and 434 cI
ACCESSION   BBa_K327002
VERSION     BBa_K327002.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..687
                     /label=cI 434
     misc_feature    649..687
                     /label=LVA
     misc_feature    19..687
                     /label=BBa_C0052
     misc_feature    688..712
                     /label=Help:Barcodes
     CDS             19..687
                     /label=BBa_C0052
     misc_feature    721..732
                     /label=B0034
     CDS             742..984
                     /label=phiR73 delta
     misc_feature    742..742
                     /label=phiR73 delta
     misc_feature    822..822
                     /label=silent mutation to remove PstI site
     misc_feature    721..984
                     /label=BBa_I746352
     misc_feature    997..1000
                     /label=conserved
     RBS             993..1004
                     /label=BBa_B0034
     CDS             1014..1630
                     /label=tetR
     misc_feature    1631..1664
                     /label=SsrA
     misc_feature    1671..1695
                     /label=Help:Barcodes
     CDS             1011..1695
                     /label=BBa_C0040
     stem_loop       1715..1758
                     /label=stem_loop
     misc_feature    1704..1783
                     /label=BBa_B0010
     terminator      1704..1783
                     /label=BBa_B0010
     stem_loop       1799..1818
                     /label=T7 TE
     misc_feature    1825..1825
                     /label=stop
     polyA_site      1819..1832
                     /label=polya
     misc_feature    1792..1832
                     /label=BBa_B0012
     terminator      1792..1832
                     /label=BBa_B0012
     terminator      1704..1832
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gagtatttct tccagggtaa aaagcaaaag aatccagctt
       61 ggacttaacc aggctgaact tgctcaaaag gtggggacta cccagcagtc tatagagcag
      121 ctcgaaaacg gtaaaactaa gcgaccacgc tttttaccag aacttgcgtc agctcttggc
      181 gtaagtgttg actggctgct caatggcacc tctgattcga atgttagatt tgttgggcac
      241 gttgagccca aagggaaata tccattgatt agcatggtta gagctggttc gtggtgtgaa
      301 gcttgtgaac cctacgatat caaggacatt gatgaatggt atgacagtga cgttaactta
      361 ttaggcaatg gattctggct gaaggttgaa ggtgattcca tgacctcacc tgtaggtcaa
      421 agcatccctg aaggtcatat ggtgttagta gatactggac gggagccagt gaatggaagc
      481 cttgttgtag ccaaactgac tgacgcgaac gaagcaacat tcaagaaact ggtcatagat
      541 ggcggtcaga agtacctgaa aggcctgaat ccttcatggc ctatgactcc tatcaacgga
      601 aactgcaaga ttatcggtgt tgtcgtggaa gcgagggtaa aattcgtagc tgcaaacgac
      661 gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag
      721 aaagaggaga aatactagat gatgcgctgc cctttctgtc gtcattcagc gcatacccgc
      781 accagccggt atgtgagtga caatgtcaaa gaaagttatc tccagtgcca gaatatttac
      841 tgttcggcga catttaaaac gcatgagtca atttgtgccg tgattcgttc tccggtcacg
      901 gaggaaaaac cagcaccggc aagcacagca ccggctgttg tccgaaaagt taaaggctgt
      961 tacagctcac cattcaacca ttaatactag agaaagagga gaaatactag atgtccagat
     1021 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
     1081 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
     1141 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
     1201 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
     1261 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
     1321 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
     1381 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
     1441 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
     1501 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     1561 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     1621 aaagtgggtc cgctgcaaac gacgaaaact acgctttagt agcttaataa cactgatagt
     1681 gctagtgtag atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     1741 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     1801 ctcaccttcg ggtgggcctt tctgcgttta ta
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