LOCUS       BBa_K327004             1074 bp    DNA     linear       29-Nov-2024
DEFINITION  pBad Promoter+ RBS + Las Membrane Protein + double terminator
ACCESSION   BBa_K327004
VERSION     BBa_K327004.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             157..873
                     /label=lasR
     misc_feature    874..906
                     /label=LVA
     misc_feature    636..636
                     /label=G
     misc_feature    913..937
                     /label=Help:Barcodes
     CDS             157..937
                     /label=BBa_C0079
     stem_loop       957..1000
                     /label=stem_loop
     misc_feature    946..1025
                     /label=BBa_B0010
     terminator      946..1025
                     /label=BBa_B0010
     stem_loop       1041..1060
                     /label=T7 TE
     misc_feature    1067..1067
                     /label=stop
     polyA_site      1061..1074
                     /label=polya
     misc_feature    1034..1074
                     /label=BBa_B0012
     terminator      1034..1074
                     /label=BBa_B0012
     terminator      946..1074
                     /label=BBa_B0015
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg ccttggttga cggttttctt
      181 gagctggaac gctcaagtgg aaaattggag tggagcgcca tcctccagaa gatggcgagc
      241 gaccttggat tctcgaagat cctgttcggc ctgttgccta aggacagcca ggactacgag
      301 aacgccttca tcgtcggcaa ctacccggcc gcctggcgcg agcattacga ccgggctggc
      361 tacgcgcggg tcgacccgac ggtcagtcac tgtacccaga gcgtactgcc gattttctgg
      421 gaaccgtcca tctaccagac gcgaaagcag cacgagttct tcgaggaagc ctcggccgcc
      481 ggcctggtgt atgggctgac catgccgctg catggtgctc gcggcgaact cggcgcgctg
      541 agcctcagcg tggaagcgga aaaccgggcc gaggccaacc gtttcataga gtcggtcctg
      601 ccgaccctgt ggatgctcaa ggactacgca ctgcaaagcg gtgccggact ggccttcgaa
      661 catccggtca gcaaaccggt ggttctgacc agccgggaga aggaagtgtt gcagtggtgc
      721 gccatcggca agaccagttg ggagatatcg gttatctgca actgctcgga agccaatgtg
      781 aacttccata tgggaaatat tcggcggaag ttcggtgtga cctcccgccg cgtagcggcc
      841 attatggccg ttaatttggg tcttattact ctcgctgcaa acgacgaaaa ctacgcttta
      901 gtagcttaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa
      961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1021 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//