LOCUS       BBa_K327008             1778 bp    DNA     linear       22-Nov-2024
DEFINITION  The initiator testing construct (initiator with RFP)
ACCESSION   BBa_K327008
VERSION     BBa_K327008.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     misc_feature    121..124
                     /label=conserved
     RBS             117..128
                     /label=BBa_B0034
     CDS             135..809
                     /label=mrfp1
     misc_feature    816..840
                     /label=Help:Barcodes
     CDS             135..840
                     /label=BBa_E1010
     misc_feature    853..856
                     /label=conserved
     RBS             849..860
                     /label=BBa_B0034
     CDS             870..1577
                     /label=cI lambda
     misc_feature    1578..1610
                     /label=LVA
     misc_feature    1617..1641
                     /label=Help:Barcodes
     CDS             867..1641
                     /label=BBa_C0051
     stem_loop       1661..1704
                     /label=stem_loop
     misc_feature    1650..1729
                     /label=BBa_B0010
     terminator      1650..1729
                     /label=BBa_B0010
     stem_loop       1745..1764
                     /label=T7 TE
     misc_feature    1771..1771
                     /label=stop
     polyA_site      1765..1778
                     /label=polya
     misc_feature    1738..1778
                     /label=BBa_B0012
     terminator      1738..1778
                     /label=BBa_B0012
     terminator      1650..1778
                     /label=BBa_B0015
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag
      121 aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag
      181 ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc
      241 gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt
      301 tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc
      361 cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg
      421 ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg
      481 gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta
      541 tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg
      601 ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg
      661 aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg
      721 acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac
      781 gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc
      841 tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta acacaagagc
      901 agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct
      961 tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgctttat
     1021 ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa attctcaaag
     1081 ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg
     1141 ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg
     1201 cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag agatgggtaa
     1261 gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga
     1321 ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc gttgaccctg
     1381 agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat gagtttacct
     1441 tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac ccacagtacc
     1501 caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct agtcagtggc
     1561 ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct taataacgct
     1621 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1681 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1741 cactggctca ccttcgggtg ggcctttctg cgtttata
//