LOCUS BBa_K327010 6100 bp DNA linear 25-Nov-2024 DEFINITION light sensor + RFP + lambda cI ACCESSION BBa_K327010 VERSION BBa_K327010.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 misc_feature 883..907 /label=Help:Barcodes CDS 157..907 /label=BBa_I15008 misc_feature 920..923 /label=conserved RBS 916..927 /label=BBa_B0034 CDS 934..1683 /label=PcyA misc_feature 1684..1708 /label=Help:Barcodes CDS 934..1708 /label=BBa_I15009 stem_loop 1728..1771 /label=stem_loop misc_feature 1717..1796 /label=BBa_B0010 terminator 1717..1796 /label=BBa_B0010 stem_loop 1812..1831 /label=T7 TE misc_feature 1838..1838 /label=stop polyA_site 1832..1845 /label=polya misc_feature 1805..1845 /label=BBa_B0012 terminator 1805..1845 /label=BBa_B0012 terminator 1717..1845 /label=BBa_B0015 promoter 1854..1888 /label=BBa_J23119 misc_feature 1901..1904 /label=conserved RBS 1897..1908 /label=BBa_B0034 CDS 1915..4152 /label=Cph8 misc_feature 4153..4177 /label=Help:Barcodes CDS 1915..4177 /label=BBa_I15010 stem_loop 4197..4240 /label=stem_loop misc_feature 4186..4265 /label=BBa_B0010 terminator 4186..4265 /label=BBa_B0010 stem_loop 4281..4300 /label=T7 TE misc_feature 4307..4307 /label=stop polyA_site 4301..4314 /label=polya misc_feature 4274..4314 /label=BBa_B0012 terminator 4274..4314 /label=BBa_B0012 terminator 4186..4314 /label=BBa_B0015 misc_feature 1..4314 /label=BBa_K327006 misc_feature 4335..4352 /label=C1 OmpR misc_feature 4356..4373 /label=C2 OmpR misc_feature 4376..4393 /label=C3 OmpR promoter 4397..4402 /label=-35 promoter 4420..4425 /label=-10 promoter 4323..4430 /label=BBa_R0082 misc_feature 4443..4446 /label=conserved RBS 4439..4450 /label=BBa_B0034 CDS 4457..5131 /label=mrfp1 misc_feature 5138..5162 /label=Help:Barcodes CDS 4457..5162 /label=BBa_E1010 misc_feature 5175..5178 /label=conserved RBS 5171..5182 /label=BBa_B0034 CDS 5192..5899 /label=cI lambda misc_feature 5900..5932 /label=LVA misc_feature 5939..5963 /label=Help:Barcodes CDS 5189..5963 /label=BBa_C0051 stem_loop 5983..6026 /label=stem_loop misc_feature 5972..6051 /label=BBa_B0010 terminator 5972..6051 /label=BBa_B0010 stem_loop 6067..6086 /label=T7 TE misc_feature 6093..6093 /label=stop polyA_site 6087..6100 /label=polya misc_feature 6060..6100 /label=BBa_B0012 terminator 6060..6100 /label=BBa_B0012 terminator 5972..6100 /label=BBa_B0015 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga gtgtcaactt agcttcccag 181 ttgcgggaag ggacgaaaaa atcccactcc atggcggaga acgtcggctt tgtcaaatgc 241 ttcctcaagg gcgttgtcga gaaaaattcc taccgtaagc tggttggcaa tctctacttt 301 gtctacagtg ccatggaaga ggaaatggca aaatttaagg accatcccat cctcagccac 361 atttacttcc ccgaactcaa ccgcaaacaa agcctagagc aagacctgca attctattac 421 ggctccaact ggcggcaaga agtgaaaatt tctgccgctg gccaagccta tgtggaccga 481 gtccggcaag tggccgctac ggcccctgaa ttgttggtgg cccattccta cacccgttac 541 ctgggggatc tttccggcgg tcaaattctc aagaaaattg cccaaaatgc catgaatctc 601 cacgatggtg gcacagcttt ctatgaattt gccgacattg atgacgaaaa ggcttttaaa 661 aatacctacc gtcaagctat gaatgatctg cccattgacc aagccaccgc cgaacggatt 721 gtggatgaag ccaatgacgc ctttgccatg aacatgaaaa tgttcaacga acttgaaggc 781 aacctgatca aggcgatcgg cattatggtg ttcaacagcc tcacccgtcg ccgcagtcaa 841 ggcagcaccg aagttggcct cgccacctcc gaaggctaat aacgctgata gtgctagtgt 901 agatcgctac tagagaaaga ggagaaatac tagatggccg tcactgattt aagtttgacc 961 aattcttccc tgatgcctac gttgaacccg atgattcaac agttggccct ggcgatcgcc 1021 gctagttggc aaagtttacc cctcaagccc tatcaattgc cggaggattt gggctacgta 1081 gaaggccgcc tggaagggga aaagttagtg attgaaaatc ggtgctacca aacgccccag 1141 tttcgcaaaa tgcatttgga gttggccaag gtgggcaaag ggttggatat tctccactgt 1201 gtaatgtttc ctgagccttt atacggtcta cctttgtttg gctgtgacat tgtggccggc 1261 cccggtggag taagtgcggc tattgcggat ctatccccca cccaaagcga tcgccaattg 1321 cccgcagcgt accaaaaatc attggcagag ctaggccagc cagaatttga gcaacaacgg 1381 gaattgcccc cctggggaga aatattttct gaatattgtt tattcatccg tcccagcaat 1441 gtcactgaag aagaaagatt tgtacaaagg gtagtggact ttttgcaaat tcattgtcac 1501 caatccatcg ttgccgaacc cttgtctgaa gctcaaactt tggagcaccg tcaggggcaa 1561 attcattact gccaacaaca acagaaaaat gataaaaccc gtcgggtact ggaaaaagct 1621 tttggggaag cttgggcgga acggtatatg agccaagtct tatttgatgt tatccaataa 1681 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg 1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1801 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagttgacag 1861 ctagctcagt cctaggtata atgctagcta ctagagaaag aggagaaata ctagatggcc 1921 accaccgtac aactcagcga ccaatccctc cgtcagctag aaaccctcgc catccacacc 1981 gcccacctga ttcagcccca cggtttagtg gtggtcctgc aagaaccaga cctcaccatc 2041 agccaaatta gcgccaactg caccggcatt ttagggcgat cgccagagga tttgttgggc 2101 agaaccctag gggaagtgtt tgatagcttt cagattgatc ccatccagag tcgcctaacg 2161 gccggacaaa tcagcagcct caaccccagt aaactttggg cgcgggtcat gggggacgac 2221 tttgtcattt ttgacggggt ttttcatcgc aacagtgacg gtttattggt atgtgaactc 2281 gagccagcct acacttccga taatctgccc ttcctcggtt tttatcacat ggccaacgct 2341 gccctgaatc ggttgcgcca acaagctaat ctacgggatt tctacgatgt tattgtcgaa 2401 gaagtccgcc gtatgactgg ctttgaccgg gtgatgctat accgctttga tgaaaataac 2461 cacggtgatg tcattgccga agataaacgg gatgatatgg aaccctattt gggcctgcac 2521 tatcccgaat cggatattcc ccaacccgcc cgtcggctat ttatccacaa ccccattcga 2581 gtaattcccg atgtttatgg tgtggcggtg cccctgaccc cagcggttaa ccccagcacc 2641 aaccgagcgg tggatttaac agaatccatt ctgcgcagtg cgtaccattg ccacttgacc 2701 tatctgaaaa atatgggggt aggagcgtct ttaaccattt ccctaattaa ggacggccat 2761 ctctgggggc tcattgcctg ccaccatcaa acccccaaag taattccctt tgaactgcgt 2821 aaagcctgcg aattttttgg tcgggtggtg tttagcaaca tttccgccca ggaagatacg 2881 gaaaccttcg attaccgggt gcagctggcg gagcatgaag cggttttatt ggacaaaatg 2941 accacggcgg cggattttgt cgaaggatta actaatcatc ccgatcgcct gttgggatta 3001 acgggctccc agggggcggc catttgcttt ggggaaaaat tgattttagt aggggaaacc 3061 ccggacgaga aagcagtgca atatttactg caatggttgg agaatcggga agtgcaagac 3121 gttttcttca cctcttccct ctcacaaatt tatcctgatg cagtgaattt taaatccgtg 3181 gccagtggct tattggccat tcccattgcc cgtcacaact ttttgctctg gtttcgccct 3241 gaagtgttgc aaacggttaa ttggggcggt gacccaaatc atgcttacga agctacccag 3301 gaagacggta aaatcgagct ccatccccgc caatcctttg acctctggaa agaaattgtc 3361 cgactccaat ctttgccctg gcaatcggtg gaaatccaaa gtgccctggc cctgaaaaag 3421 gcgatcgtca acctcatttt gcgccaggca gaagaattgc atatggcggc tggtgttaag 3481 caactggcgg atgaccgcac gctgctgatg gcgggggtaa gtcacgactt gcgcacgccg 3541 ctgacgcgta ttcgcctggc gactgagatg atgagcgagc aggatggcta tctggcagaa 3601 tcgatcaata aagatatcga agagtgcaac gccatcattg agcagtttat cgactacctg 3661 cgcaccgggc aggagatgcc gatggaaatg gcggatctta atgcagtact cggtgaggtg 3721 attgctgccg aaagtggcta tgagcgggaa attgaaaccg cgctttaccc cggcagcatt 3781 gaagtgaaaa tgcacccgct gtcgatcaaa cgcgcggtgg cgaatatggt ggtcaacgcc 3841 gcccgttatg gcaatggctg ggtcaaagtc agcagcggaa cggagccgaa tcgcgcctgg 3901 ttccaggtgg aagatgacgg tccgggaatt gcgccggaac aacgtaagca cctgttccag 3961 ccgtttgtcc gcggcgacag tgcgcgcacc attagcggca cgggattagg gctggcaatt 4021 gtgcagcgta tcgtggataa ccataacggg atgctggagc ttggcaccag cgagcggggc 4081 gggctttcca ttcgcgcctg gctgccagtg ccggtaacgc gggcgcaggg catgacaaaa 4141 gaagggtaat aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa 4201 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 4261 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 4321 agtcccttgc atttacattt tgaaacatct atagcgataa atgaaacatc ttaaaagttt 4381 tagtatcata ttcgtgttgg attattctgc atttttgggg agaatggact tactagagaa 4441 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa 4501 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg 4561 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc 4621 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca 4681 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg 4741 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga 4801 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt 4861 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg 4921 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc 4981 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac 5041 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga 5101 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc 5161 gctactagag aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga 5221 gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg 5281 cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt 5341 atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa 5401 agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc 5461 ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca 5521 ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt 5581 aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat 5641 gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc 5701 tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac 5761 cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta 5821 cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg 5881 gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg 5941 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag 6001 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 6061 cacactggct caccttcggg tgggcctttc tgcgtttata //