##gff-version 3
##sequence-region BBa_K327010 1 6100
BBa_K327010	.	CDS	4457	5162	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K327010	.	engineered_tag	5138	5162	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K327010	.	CDS	4457	5131	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K327010	.	ribosome_entry_site	4439	4450	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K327010	.	conserved_region	4443	4446	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327010	.	terminator	5972	6100	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K327010	.	terminator	6060	6100	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K327010	.	polyA_site	6087	6100	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K327010	.	stem_loop	6067	6086	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K327010	.	stop_codon	6093	6093	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K327010	.	engineered_region	6060	6100	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K327010	.	terminator	5972	6051	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K327010	.	stem_loop	5983	6026	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K327010	.	engineered_region	5972	6051	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K327010	.	promoter	4323	4430	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K327010	.	promoter	4420	4425	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K327010	.	non_covalent_binding_site	4356	4373	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K327010	.	non_covalent_binding_site	4335	4352	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K327010	.	promoter	4397	4402	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K327010	.	non_covalent_binding_site	4376	4393	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K327010	.	engineered_region	1	4314	.	+	0	ID=BBa_K327006;Name=BBa_K327006
BBa_K327010	.	CDS	157	907	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_K327006
BBa_K327010	.	engineered_tag	883	907	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_K327010	.	terminator	1717	1845	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K327006
BBa_K327010	.	terminator	1805	1845	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K327010	.	polyA_site	1832	1845	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K327010	.	stem_loop	1812	1831	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K327010	.	stop_codon	1838	1838	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K327010	.	engineered_region	1805	1845	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K327010	.	terminator	1717	1796	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K327010	.	stem_loop	1728	1771	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K327010	.	engineered_region	1717	1796	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K327010	.	CDS	1915	4177	.	+	0	ID=BBa_I15010;Name=BBa_I15010;Parent=BBa_K327006
BBa_K327010	.	engineered_tag	4153	4177	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_K327010	.	CDS	1915	4152	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_K327010	.	ribosome_entry_site	1897	1908	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327006
BBa_K327010	.	conserved_region	1901	1904	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327010	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K327006
BBa_K327010	.	terminator	4186	4314	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K327006
BBa_K327010	.	terminator	4274	4314	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K327010	.	polyA_site	4301	4314	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K327010	.	stem_loop	4281	4300	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K327010	.	stop_codon	4307	4307	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K327010	.	engineered_region	4274	4314	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K327010	.	terminator	4186	4265	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K327010	.	stem_loop	4197	4240	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K327010	.	engineered_region	4186	4265	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K327010	.	promoter	1854	1888	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K327006
BBa_K327010	.	CDS	934	1708	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_K327006
BBa_K327010	.	CDS	934	1683	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_K327010	.	engineered_tag	1684	1708	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_K327010	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327006
BBa_K327010	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327010	.	ribosome_entry_site	916	927	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327006
BBa_K327010	.	conserved_region	920	923	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327010	.	ribosome_entry_site	5171	5182	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K327010	.	conserved_region	5175	5178	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327010	.	CDS	5189	5963	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K327010	.	tag	5900	5932	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K327010	.	CDS	5192	5899	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K327010	.	engineered_tag	5939	5963	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
>BBa_K327010
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaa
tgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatgg
aagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcct
agagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtg
gaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatcttt
ccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgc
cgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgcc
gaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatca
aggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctc
cgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgat
ttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagtt
ggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagtt
agtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttg
gatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccg
gtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcatt
ggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgttta
ttcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcacc
aatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaaca
acagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtc
ttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagag
aaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatcc
acaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgc
caactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcag
attgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgg
gggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagccta
cacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaat
ctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttg
atgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccga
atcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcg
gtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtacc
attgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctg
ggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgg
gtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcgg
ttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaac
gggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaa
tatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatg
cagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccc
tgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgag
ctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatcc
aaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgt
taagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgc
ctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgcca
tcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcgg
tgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatg
cacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtca
gcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagca
cctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgt
atcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccag
tgccggtaacgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagtgtagatctctactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccttgcatttacattttgaaa
catctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaa
tggacttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagt
tcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccag
accgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggtt
ccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacg
tgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaa
gttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccg
aacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacga
cgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggac
atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaat
aacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaac
acaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaa
tctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttata
acgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagat
gtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatg
ttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctg
cattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaat
tctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaag
aaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagtt
gttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgcttt
agtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttata