##gff-version 3
##sequence-region BBa_K327011 1 6484
BBa_K327011	.	engineered_region	1	1108	.	+	0	ID=BBa_K327009;Name=BBa_K327009
BBa_K327011	.	promoter	1	170	.	+	0	ID=BBa_I14015;Name=BBa_I14015;Parent=BBa_K327009
BBa_K327011	.	promoter	117	122	.	+	0	ID=annotation1026120;Name=-35;Parent=BBa_I14015
BBa_K327011	.	engineered_region	1	151	.	+	0	ID=annotation1017909;Name=R0079;Parent=BBa_I14015
BBa_K327011	.	promoter	140	145	.	+	0	ID=annotation1026116;Name=-10;Parent=BBa_I14015
BBa_K327011	.	non_covalent_binding_site	152	170	.	+	0	ID=annotation1017910;Name=TetR;Parent=BBa_I14015
BBa_K327011	.	ribosome_entry_site	179	190	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327009
BBa_K327011	.	conserved_region	183	186	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327011	.	CDS	197	971	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K327009
BBa_K327011	.	tag	908	940	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K327011	.	engineered_tag	947	971	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K327011	.	CDS	200	907	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K327011	.	terminator	980	1108	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K327009
BBa_K327011	.	terminator	980	1059	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K327011	.	stem_loop	991	1034	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K327011	.	engineered_region	980	1059	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K327011	.	terminator	1068	1108	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K327011	.	stem_loop	1075	1094	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K327011	.	stop_codon	1101	1101	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K327011	.	polyA_site	1095	1108	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K327011	.	engineered_region	1068	1108	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K327011	.	engineered_region	5439	6484	.	+	0	ID=BBa_K327007;Name=BBa_K327007
BBa_K327011	.	ribosome_entry_site	5555	5566	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327007
BBa_K327011	.	conserved_region	5559	5562	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327011	.	CDS	5573	6347	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K327007
BBa_K327011	.	tag	6284	6316	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K327011	.	engineered_tag	6323	6347	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K327011	.	CDS	5576	6283	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K327011	.	terminator	6356	6484	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K327007
BBa_K327011	.	terminator	6356	6435	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K327011	.	stem_loop	6367	6410	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K327011	.	engineered_region	6356	6435	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K327011	.	terminator	6444	6484	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K327011	.	stem_loop	6451	6470	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K327011	.	stop_codon	6477	6477	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K327011	.	polyA_site	6471	6484	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K327011	.	engineered_region	6444	6484	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K327011	.	promoter	5439	5546	.	+	0	ID=BBa_R0082;Name=BBa_R0082;Parent=BBa_K327007
BBa_K327011	.	non_covalent_binding_site	5492	5509	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K327011	.	promoter	5536	5541	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K327011	.	non_covalent_binding_site	5472	5489	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K327011	.	non_covalent_binding_site	5451	5468	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K327011	.	promoter	5513	5518	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K327011	.	engineered_region	1117	5430	.	+	0	ID=BBa_K327006;Name=BBa_K327006
BBa_K327011	.	ribosome_entry_site	3013	3024	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327006
BBa_K327011	.	conserved_region	3017	3020	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327011	.	CDS	1273	2023	.	+	0	ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_K327006
BBa_K327011	.	engineered_tag	1999	2023	.	+	0	ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008
BBa_K327011	.	ribosome_entry_site	1255	1266	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327006
BBa_K327011	.	conserved_region	1259	1262	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327011	.	promoter	2970	3004	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K327006
BBa_K327011	.	promoter	1117	1246	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K327006
BBa_K327011	.	CDS	3031	5293	.	+	0	ID=BBa_I15010;Name=BBa_I15010;Parent=BBa_K327006
BBa_K327011	.	engineered_tag	5269	5293	.	+	0	ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010
BBa_K327011	.	CDS	3031	5268	.	+	0	ID=annotation1891613;Name=Cph8;Parent=BBa_I15010
BBa_K327011	.	CDS	2050	2824	.	+	0	ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_K327006
BBa_K327011	.	CDS	2050	2799	.	+	0	ID=annotation1891612;Name=PcyA;Parent=BBa_I15009
BBa_K327011	.	engineered_tag	2800	2824	.	+	0	ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009
BBa_K327011	.	terminator	2833	2961	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K327006
BBa_K327011	.	terminator	2833	2912	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K327011	.	stem_loop	2844	2887	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K327011	.	engineered_region	2833	2912	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K327011	.	terminator	2921	2961	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K327011	.	stem_loop	2928	2947	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K327011	.	stop_codon	2954	2954	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K327011	.	polyA_site	2948	2961	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K327011	.	engineered_region	2921	2961	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K327011	.	ribosome_entry_site	2032	2043	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327006
BBa_K327011	.	conserved_region	2036	2039	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327011	.	terminator	5302	5430	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K327006
BBa_K327011	.	terminator	5302	5381	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K327011	.	stem_loop	5313	5356	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K327011	.	engineered_region	5302	5381	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K327011	.	terminator	5390	5430	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K327011	.	stem_loop	5397	5416	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K327011	.	stop_codon	5423	5423	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K327011	.	polyA_site	5417	5430	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K327011	.	engineered_region	5390	5430	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K327011
gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaaca
tacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacactccctat
cagtgatagagatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttga
ggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaag
atggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttg
caaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttag
tatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagctt
agaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgagg
ttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctga
gcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggat
agcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtgggga
aagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacg
ctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttc
gttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttta
tatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatc
ctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggaga
aatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcgg
ctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctac
agtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgca
aacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggcca
agcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctg
ggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttct
atgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgacca
agccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggc
aacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcc
tcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggc
cgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatc
gccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaag
gggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtggg
caaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtg
gccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtacc
aaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctga
atattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaatt
cattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattact
gccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatat
gagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgcta
gctactagagaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaacc
ctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagcc
aaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttga
tagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcg
cgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcg
agccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgcca
acaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgcta
taccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgc
actatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgttta
tggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgc
agtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacg
gccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaatt
ttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggag
catgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgt
tgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaa
agcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatt
tatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctct
ggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacgg
taaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcg
gtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatgg
cggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgac
gcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagag
tgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatg
cagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattga
agtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgg
gtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaac
aacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaat
tgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcc
tggctgccagtgccggtaacgcgggcgcagggcatgacaaaagaagggtaataactctgatagtgctagtgtagatctc
tactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtg
aacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccttgcattta
cattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcattt
ttggggagaatggacttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagc
ttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcaga
caagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattg
cttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcgg
ttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctga
gcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggctt
gaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgacc
ctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcag
ggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtg
gggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaat
aacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata