LOCUS       BBa_K327012             2421 bp    DNA     linear       25-Nov-2024
DEFINITION  cI lam activated RFP, cI lam, and Lux signal
ACCESSION   BBa_K327012
VERSION     BBa_K327012.1  
FEATURES             Location/Qualifiers
     misc_feature    56..69
                     /label=OR1 434
     misc_feature    9..25
                     /label=OR1 lambda
     misc_feature    33..49
                     /label=OR2 lambda
     promoter        71..76
                     /label=-10
     promoter        48..53
                     /label=-35
     promoter        1..82
                     /label=BBa_I12006
     misc_feature    95..98
                     /label=conserved
     RBS             91..102
                     /label=BBa_B0034
     CDS             112..819
                     /label=cI lambda
     misc_feature    820..852
                     /label=LVA
     misc_feature    859..883
                     /label=Help:Barcodes
     CDS             109..883
                     /label=BBa_C0051
     misc_feature    896..899
                     /label=conserved
     RBS             892..903
                     /label=BBa_B0034
     CDS             910..1584
                     /label=mrfp1
     misc_feature    1591..1615
                     /label=Help:Barcodes
     CDS             910..1615
                     /label=BBa_E1010
     misc_feature    1628..1631
                     /label=conserved
     RBS             1624..1635
                     /label=BBa_B0034
     misc_feature    2221..2252
                     /label=LVA
     CDS             1642..2220
                     /label=luxI
     misc_feature    1642..2259
                     /label=BBa_C0061
     misc_feature    2260..2284
                     /label=Help:Barcodes
     CDS             1642..2259
                     /label=BBa_C0061
     stem_loop       2304..2347
                     /label=stem_loop
     misc_feature    2293..2372
                     /label=BBa_B0010
     terminator      2293..2372
                     /label=BBa_B0010
     stem_loop       2388..2407
                     /label=T7 TE
     misc_feature    2414..2414
                     /label=stop
     polyA_site      2408..2421
                     /label=polya
     misc_feature    2381..2421
                     /label=BBa_B0012
     terminator      2381..2421
                     /label=BBa_B0012
     misc_feature    1624..2421
                     /label=BBa_F1610
     misc_feature    91..2421
                     /label=BBa_K327001
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atattacaaa
       61 ctttcttgta tagatttaac gttactagag aaagaggaga aatactagat gagcacaaaa
      121 aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc aatttatgaa
      181 aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat ggggatgggg
      241 cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta taacgccgca
      301 ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat cgccagagaa
      361 atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga gtatgagtac
      421 cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac ctttaccaaa
      481 ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc attctggctt
      541 gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt tcctgacgga
      601 atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg catagccaga
      661 cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca ggtgttttta
      721 caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc cgttgtgggg
      781 aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga cgaaaactac
      841 gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga gaaagaggag
      901 aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt
      961 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg
     1021 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct
     1081 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct
     1141 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg
     1201 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag
     1261 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag
     1321 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg
     1381 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt
     1441 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc
     1501 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct
     1561 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta
     1621 gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt tttggcaatt
     1681 ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa gcaaagactt
     1741 gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa ctcaaatgca
     1801 gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg tttattacct
     1861 acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca acagagtgct
     1921 cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa tagctcaaag
     1981 ataaataact ctgctagtga aattacaatg aaactatttg aagctatata taaacacgct
     2041 gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga gcgattttta
     2101 aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt attaggtgat
     2161 actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc agtcttaaat
     2221 gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga
     2281 tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2341 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2401 gtgggccttt ctgcgtttat a
//