LOCUS BBa_K327013 5546 bp DNA linear 14-Nov-2024 DEFINITION The initiator ACCESSION BBa_K327013 VERSION BBa_K327013.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 117..267 /label=R0079 misc_feature 268..286 /label=TetR promoter 256..261 /label=-10 promoter 233..238 /label=-35 promoter 117..286 /label=BBa_I14015 misc_feature 299..302 /label=conserved RBS 295..306 /label=BBa_B0034 CDS 316..1023 /label=cI lambda misc_feature 1024..1056 /label=LVA misc_feature 1063..1087 /label=Help:Barcodes CDS 313..1087 /label=BBa_C0051 stem_loop 1107..1150 /label=stem_loop misc_feature 1096..1175 /label=BBa_B0010 terminator 1096..1175 /label=BBa_B0010 stem_loop 1191..1210 /label=T7 TE misc_feature 1217..1217 /label=stop polyA_site 1211..1224 /label=polya misc_feature 1184..1224 /label=BBa_B0012 terminator 1184..1224 /label=BBa_B0012 terminator 1096..1224 /label=BBa_B0015 promoter 1233..1362 /label=BBa_I13453 misc_feature 1375..1378 /label=conserved RBS 1371..1382 /label=BBa_B0034 misc_feature 2115..2139 /label=Help:Barcodes CDS 1389..2139 /label=BBa_I15008 misc_feature 2152..2155 /label=conserved RBS 2148..2159 /label=BBa_B0034 CDS 2166..2915 /label=PcyA misc_feature 2916..2940 /label=Help:Barcodes CDS 2166..2940 /label=BBa_I15009 stem_loop 2960..3003 /label=stem_loop misc_feature 2949..3028 /label=BBa_B0010 terminator 2949..3028 /label=BBa_B0010 stem_loop 3044..3063 /label=T7 TE misc_feature 3070..3070 /label=stop polyA_site 3064..3077 /label=polya misc_feature 3037..3077 /label=BBa_B0012 terminator 3037..3077 /label=BBa_B0012 terminator 2949..3077 /label=BBa_B0015 promoter 3086..3120 /label=BBa_J23119 misc_feature 3133..3136 /label=conserved RBS 3129..3140 /label=BBa_B0034 CDS 3147..5384 /label=Cph8 misc_feature 5385..5409 /label=Help:Barcodes CDS 3147..5409 /label=BBa_I15010 stem_loop 5429..5472 /label=stem_loop misc_feature 5418..5497 /label=BBa_B0010 terminator 5418..5497 /label=BBa_B0010 stem_loop 5513..5532 /label=T7 TE misc_feature 5539..5539 /label=stop polyA_site 5533..5546 /label=polya misc_feature 5506..5546 /label=BBa_B0012 terminator 5506..5546 /label=BBa_B0012 terminator 5418..5546 /label=BBa_B0015 misc_feature 1233..5546 /label=BBa_K327006 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagaggccc 121 ctcgctgagc gcgtcccgga gctgggggca acctagctgc cacctgcttt tctgctagct 181 attccagcga aaacatacag atttccggcg aaatcaaggc tacctgccag ttctggcagg 241 tttggccgcg ggttcttttt ggtacactcc ctatcagtga tagagatact agagaaagag 301 gagaaatact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca 361 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct 421 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat 481 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa 541 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg 601 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca 661 cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa 721 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc 781 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag 841 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc 901 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc 961 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt 1021 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt 1081 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 1141 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 1201 cgggtgggcc tttctgcgtt tatatactag agacattgat tatttgcacg gcgtcacact 1261 ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac gctttttatc 1321 gcaactctct actgtttctc cataccgttt ttttgggcta gctactagag aaagaggaga 1381 aatactagat gagtgtcaac ttagcttccc agttgcggga agggacgaaa aaatcccact 1441 ccatggcgga gaacgtcggc tttgtcaaat gcttcctcaa gggcgttgtc gagaaaaatt 1501 cctaccgtaa gctggttggc aatctctact ttgtctacag tgccatggaa gaggaaatgg 1561 caaaatttaa ggaccatccc atcctcagcc acatttactt ccccgaactc aaccgcaaac 1621 aaagcctaga gcaagacctg caattctatt acggctccaa ctggcggcaa gaagtgaaaa 1681 tttctgccgc tggccaagcc tatgtggacc gagtccggca agtggccgct acggcccctg 1741 aattgttggt ggcccattcc tacacccgtt acctggggga tctttccggc ggtcaaattc 1801 tcaagaaaat tgcccaaaat gccatgaatc tccacgatgg tggcacagct ttctatgaat 1861 ttgccgacat tgatgacgaa aaggctttta aaaataccta ccgtcaagct atgaatgatc 1921 tgcccattga ccaagccacc gccgaacgga ttgtggatga agccaatgac gcctttgcca 1981 tgaacatgaa aatgttcaac gaacttgaag gcaacctgat caaggcgatc ggcattatgg 2041 tgttcaacag cctcacccgt cgccgcagtc aaggcagcac cgaagttggc ctcgccacct 2101 ccgaaggcta ataacgctga tagtgctagt gtagatcgct actagagaaa gaggagaaat 2161 actagatggc cgtcactgat ttaagtttga ccaattcttc cctgatgcct acgttgaacc 2221 cgatgattca acagttggcc ctggcgatcg ccgctagttg gcaaagttta cccctcaagc 2281 cctatcaatt gccggaggat ttgggctacg tagaaggccg cctggaaggg gaaaagttag 2341 tgattgaaaa tcggtgctac caaacgcccc agtttcgcaa aatgcatttg gagttggcca 2401 aggtgggcaa agggttggat attctccact gtgtaatgtt tcctgagcct ttatacggtc 2461 tacctttgtt tggctgtgac attgtggccg gccccggtgg agtaagtgcg gctattgcgg 2521 atctatcccc cacccaaagc gatcgccaat tgcccgcagc gtaccaaaaa tcattggcag 2581 agctaggcca gccagaattt gagcaacaac gggaattgcc cccctgggga gaaatatttt 2641 ctgaatattg tttattcatc cgtcccagca atgtcactga agaagaaaga tttgtacaaa 2701 gggtagtgga ctttttgcaa attcattgtc accaatccat cgttgccgaa cccttgtctg 2761 aagctcaaac tttggagcac cgtcaggggc aaattcatta ctgccaacaa caacagaaaa 2821 atgataaaac ccgtcgggta ctggaaaaag cttttgggga agcttgggcg gaacggtata 2881 tgagccaagt cttatttgat gttatccaat aataactctg atagtgctag tgtagatctc 2941 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 3001 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 3061 gcctttctgc gtttatatac tagagttgac agctagctca gtcctaggta taatgctagc 3121 tactagagaa agaggagaaa tactagatgg ccaccaccgt acaactcagc gaccaatccc 3181 tccgtcagct agaaaccctc gccatccaca ccgcccacct gattcagccc cacggtttag 3241 tggtggtcct gcaagaacca gacctcacca tcagccaaat tagcgccaac tgcaccggca 3301 ttttagggcg atcgccagag gatttgttgg gcagaaccct aggggaagtg tttgatagct 3361 ttcagattga tcccatccag agtcgcctaa cggccggaca aatcagcagc ctcaacccca 3421 gtaaactttg ggcgcgggtc atgggggacg actttgtcat ttttgacggg gtttttcatc 3481 gcaacagtga cggtttattg gtatgtgaac tcgagccagc ctacacttcc gataatctgc 3541 ccttcctcgg tttttatcac atggccaacg ctgccctgaa tcggttgcgc caacaagcta 3601 atctacggga tttctacgat gttattgtcg aagaagtccg ccgtatgact ggctttgacc 3661 gggtgatgct ataccgcttt gatgaaaata accacggtga tgtcattgcc gaagataaac 3721 gggatgatat ggaaccctat ttgggcctgc actatcccga atcggatatt ccccaacccg 3781 cccgtcggct atttatccac aaccccattc gagtaattcc cgatgtttat ggtgtggcgg 3841 tgcccctgac cccagcggtt aaccccagca ccaaccgagc ggtggattta acagaatcca 3901 ttctgcgcag tgcgtaccat tgccacttga cctatctgaa aaatatgggg gtaggagcgt 3961 ctttaaccat ttccctaatt aaggacggcc atctctgggg gctcattgcc tgccaccatc 4021 aaacccccaa agtaattccc tttgaactgc gtaaagcctg cgaatttttt ggtcgggtgg 4081 tgtttagcaa catttccgcc caggaagata cggaaacctt cgattaccgg gtgcagctgg 4141 cggagcatga agcggtttta ttggacaaaa tgaccacggc ggcggatttt gtcgaaggat 4201 taactaatca tcccgatcgc ctgttgggat taacgggctc ccagggggcg gccatttgct 4261 ttggggaaaa attgatttta gtaggggaaa ccccggacga gaaagcagtg caatatttac 4321 tgcaatggtt ggagaatcgg gaagtgcaag acgttttctt cacctcttcc ctctcacaaa 4381 tttatcctga tgcagtgaat tttaaatccg tggccagtgg cttattggcc attcccattg 4441 cccgtcacaa ctttttgctc tggtttcgcc ctgaagtgtt gcaaacggtt aattggggcg 4501 gtgacccaaa tcatgcttac gaagctaccc aggaagacgg taaaatcgag ctccatcccc 4561 gccaatcctt tgacctctgg aaagaaattg tccgactcca atctttgccc tggcaatcgg 4621 tggaaatcca aagtgccctg gccctgaaaa aggcgatcgt caacctcatt ttgcgccagg 4681 cagaagaatt gcatatggcg gctggtgtta agcaactggc ggatgaccgc acgctgctga 4741 tggcgggggt aagtcacgac ttgcgcacgc cgctgacgcg tattcgcctg gcgactgaga 4801 tgatgagcga gcaggatggc tatctggcag aatcgatcaa taaagatatc gaagagtgca 4861 acgccatcat tgagcagttt atcgactacc tgcgcaccgg gcaggagatg ccgatggaaa 4921 tggcggatct taatgcagta ctcggtgagg tgattgctgc cgaaagtggc tatgagcggg 4981 aaattgaaac cgcgctttac cccggcagca ttgaagtgaa aatgcacccg ctgtcgatca 5041 aacgcgcggt ggcgaatatg gtggtcaacg ccgcccgtta tggcaatggc tgggtcaaag 5101 tcagcagcgg aacggagccg aatcgcgcct ggttccaggt ggaagatgac ggtccgggaa 5161 ttgcgccgga acaacgtaag cacctgttcc agccgtttgt ccgcggcgac agtgcgcgca 5221 ccattagcgg cacgggatta gggctggcaa ttgtgcagcg tatcgtggat aaccataacg 5281 ggatgctgga gcttggcacc agcgagcggg gcgggctttc cattcgcgcc tggctgccag 5341 tgccggtaac gcgggcgcag ggcatgacaa aagaagggta ataactctga tagtgctagt 5401 gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 5461 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 5521 ttcgggtggg cctttctgcg tttata //