##gff-version 3 ##sequence-region BBa_K327013 1 5546 BBa_K327013 . terminator 1096 1224 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K327013 . terminator 1096 1175 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K327013 . stem_loop 1107 1150 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K327013 . engineered_region 1096 1175 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K327013 . terminator 1184 1224 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K327013 . polyA_site 1211 1224 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K327013 . stem_loop 1191 1210 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K327013 . engineered_region 1184 1224 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K327013 . stop_codon 1217 1217 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K327013 . CDS 313 1087 . + 0 ID=BBa_C0051;Name=BBa_C0051 BBa_K327013 . CDS 316 1023 . + 0 ID=annotation23334;Name=cI lambda;Parent=BBa_C0051 BBa_K327013 . tag 1024 1056 . + 0 ID=annotation23335;Name=LVA;Parent=BBa_C0051 BBa_K327013 . engineered_tag 1063 1087 . + 0 ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051 BBa_K327013 . engineered_region 1233 5546 . + 0 ID=BBa_K327006;Name=BBa_K327006 BBa_K327013 . CDS 3147 5409 . + 0 ID=BBa_I15010;Name=BBa_I15010;Parent=BBa_K327006 BBa_K327013 . CDS 3147 5384 . + 0 ID=annotation1891613;Name=Cph8;Parent=BBa_I15010 BBa_K327013 . engineered_tag 5385 5409 . + 0 ID=annotation2214031;Name=Help:Barcodes;Parent=BBa_I15010 BBa_K327013 . promoter 3086 3120 . + 0 ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K327006 BBa_K327013 . CDS 2166 2940 . + 0 ID=BBa_I15009;Name=BBa_I15009;Parent=BBa_K327006 BBa_K327013 . CDS 2166 2915 . + 0 ID=annotation1891612;Name=PcyA;Parent=BBa_I15009 BBa_K327013 . engineered_tag 2916 2940 . + 0 ID=annotation2214032;Name=Help:Barcodes;Parent=BBa_I15009 BBa_K327013 . terminator 5418 5546 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K327006 BBa_K327013 . terminator 5418 5497 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K327013 . stem_loop 5429 5472 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K327013 . engineered_region 5418 5497 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K327013 . terminator 5506 5546 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K327013 . polyA_site 5533 5546 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K327013 . stem_loop 5513 5532 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K327013 . engineered_region 5506 5546 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K327013 . stop_codon 5539 5539 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K327013 . CDS 1389 2139 . + 0 ID=BBa_I15008;Name=BBa_I15008;Parent=BBa_K327006 BBa_K327013 . engineered_tag 2115 2139 . + 0 ID=annotation2214026;Name=Help:Barcodes;Parent=BBa_I15008 BBa_K327013 . terminator 2949 3077 . + 0 ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K327006 BBa_K327013 . terminator 2949 3028 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K327013 . stem_loop 2960 3003 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K327013 . engineered_region 2949 3028 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K327013 . terminator 3037 3077 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K327013 . polyA_site 3064 3077 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K327013 . stem_loop 3044 3063 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K327013 . engineered_region 3037 3077 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K327013 . stop_codon 3070 3070 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K327013 . ribosome_entry_site 3129 3140 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327006 BBa_K327013 . conserved_region 3133 3136 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K327013 . ribosome_entry_site 2148 2159 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327006 BBa_K327013 . conserved_region 2152 2155 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K327013 . promoter 1233 1362 . + 0 ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K327006 BBa_K327013 . ribosome_entry_site 1371 1382 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K327006 BBa_K327013 . conserved_region 1375 1378 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K327013 . ribosome_entry_site 295 306 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K327013 . conserved_region 299 302 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K327013 . promoter 117 286 . + 0 ID=BBa_I14015;Name=BBa_I14015 BBa_K327013 . promoter 233 238 . + 0 ID=annotation1026120;Name=-35;Parent=BBa_I14015 BBa_K327013 . promoter 256 261 . + 0 ID=annotation1026116;Name=-10;Parent=BBa_I14015 BBa_K327013 . non_covalent_binding_site 268 286 . + 0 ID=annotation1017910;Name=TetR;Parent=BBa_I14015 BBa_K327013 . engineered_region 117 267 . + 0 ID=annotation1017909;Name=R0079;Parent=BBa_I14015 BBa_K327013 . promoter 1 108 . + 0 ID=BBa_R0082;Name=BBa_R0082 BBa_K327013 . non_covalent_binding_site 13 30 . + 0 ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082 BBa_K327013 . non_covalent_binding_site 34 51 . + 0 ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082 BBa_K327013 . promoter 98 103 . + 0 ID=annotation301167;Name=-10;Parent=BBa_R0082 BBa_K327013 . non_covalent_binding_site 54 71 . + 0 ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082 BBa_K327013 . promoter 75 80 . + 0 ID=annotation301166;Name=-35;Parent=BBa_R0082 >BBa_K327013 tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga ttattctgcatttttggggagaatggacttactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagct gccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggc aggtttggccgcgggttctttttggtacactccctatcagtgatagagatactagagaaagaggagaaatactagatga gcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaa tgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggc atcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaa tcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgtttt ttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcaca accaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaa gctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgg gggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtaccca atgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctg caaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaa ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc acactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgc tatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat accgtttttttgggctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaag ggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattc ctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatccc atcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctcca actggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccc tgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaa aatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaata cctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctt tgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctc acccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgta gatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctac gttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattg ccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgcccc agtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcc tttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcc cccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaac aacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaaga aagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagct caaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactgg aaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgata gtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatact agagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggccaccaccgt acaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggttta gtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccag aggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccgg acaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggttttt catcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggttttt atcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaaga agtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaa gataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctat ttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcac caaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggg gtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaaccccca aagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaaga tacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggat tttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttgggg aaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagt gcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggctta ttggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtg acccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaa agaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtc aacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctga tggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatgg ctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcacc gggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagc gggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaa tatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttc caggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtg cgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagct tggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcatgacaaaa gaagggtaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaa agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg cctttctgcgtttata