BBa_K327014
type: ComponentDefinition
persistent identity: BBa K327014
display id: BBa_K327014
version: 1
was generated by: iGEM to SBOL conversion
description: The initiator
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:03Z
mutable provenance: Parts registry
owned by: james
owned by: myers
mutable description: A piece of our genetic circuit that transcribes the cl lambda protein, activated by light OR las
discontinued: false
dominant: true
group u list: _447_
m user id: 0
owner id: 6137
owning group id: 415
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Jennifer Lau
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0034
component: BBa_I14015
component: BBa_R0082
component: BBa_K327006
component: BBa_B0015
component: BBa_C0051
sequence annotation: BBa_R0082
sequence annotation: BBa_I14015
sequence annotation: BBa_B0034
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence annotation: BBa_K327006
sequence: https://synbiohub.org/public/igem/BBa_K327014_sequence/1

BBa_B0034
type: Component
persistent identity: component2318177
display id: component2318177
version: 1
top level: BBa_K327014
definition: BBa_B0034
access: public

BBa_I14015
type: Component
persistent identity: component2318175
display id: component2318175
version: 1
top level: BBa_K327014
definition: las TetO
access: public

BBa_R0082
type: Component
persistent identity: component2318170
display id: component2318170
version: 1
top level: BBa_K327014
definition: OmpR
access: public

BBa_K327006
type: Component
persistent identity: component2318219
display id: component2318219
version: 1
top level: BBa_K327014
definition: BBa_K327006
access: public

BBa_B0015
type: Component
persistent identity: component2318188
display id: component2318188
version: 1
top level: BBa_K327014
definition: BBa_B0015
access: public

BBa_C0051
type: Component
persistent identity: component2318181
display id: component2318181
version: 1
top level: BBa_K327014
definition: cI lam
access: public

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation2318170
display id: annotation2318170
version: 1
top level: BBa_K327014
location: https://synbiohub.org/public/igem/BBa_K327014/annotation2318170/range2318170/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_K327014/annotation2318170/range2318170/1
type: Range
persistent identity: range2318170
display id: range2318170
version: 1
top level: BBa_K327014
direction: direction/reverse
start: 1
end: 108
orientation: inline

BBa_I14015
type: SequenceAnnotation
persistent identity: annotation2318175
display id: annotation2318175
version: 1
top level: BBa_K327014
location: https://synbiohub.org/public/igem/BBa_K327014/annotation2318175/range2318175/1
component: BBa_I14015

https://synbiohub.org/public/igem/BBa_K327014/annotation2318175/range2318175/1
type: Range
persistent identity: range2318175
display id: range2318175
version: 1
top level: BBa_K327014
direction: direction/reverse
start: 117
end: 286
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2318177
display id: annotation2318177
version: 1
top level: BBa_K327014
location: https://synbiohub.org/public/igem/BBa_K327014/annotation2318177/range2318177/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K327014/annotation2318177/range2318177/1
type: Range
persistent identity: range2318177
display id: range2318177
version: 1
top level: BBa_K327014
direction: direction/reverse
start: 295
end: 306
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2318181
display id: annotation2318181
version: 1
top level: BBa_K327014
location: https://synbiohub.org/public/igem/BBa_K327014/annotation2318181/range2318181/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K327014/annotation2318181/range2318181/1
type: Range
persistent identity: range2318181
display id: range2318181
version: 1
top level: BBa_K327014
direction: direction/reverse
start: 313
end: 1087
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2318188
display id: annotation2318188
version: 1
top level: BBa_K327014
location: https://synbiohub.org/public/igem/BBa_K327014/annotation2318188/range2318188/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K327014/annotation2318188/range2318188/1
type: Range
persistent identity: range2318188
display id: range2318188
version: 1
top level: BBa_K327014
direction: direction/reverse
start: 1096
end: 1224
orientation: inline

BBa_K327006
type: SequenceAnnotation
persistent identity: annotation2318219
display id: annotation2318219
version: 1
top level: BBa_K327014
location: https://synbiohub.org/public/igem/BBa_K327014/annotation2318219/range2318219/1
component: BBa_K327006

https://synbiohub.org/public/igem/BBa_K327014/annotation2318219/range2318219/1
type: Range
persistent identity: range2318219
display id: range2318219
version: 1
top level: BBa_K327014
direction: direction/reverse
start: 1233
end: 5546
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

las TetO
type: ComponentDefinition
persistent identity: BBa I14015
display id: BBa_I14015
version: 1
was generated by: iGEM to SBOL conversion
description: P(Las) TetO
created: 2004-07-29T11:00:00Z
modified: 2015-08-31T04:07:37Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: LasR and 3OC12HSL regulated promoter with a single Tet Operator (binding site for TetR repressor)
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: R0079
sequence annotation: -35
sequence annotation: -10
sequence annotation: TetR
sequence: https://synbiohub.org/public/igem/BBa_I14015_sequence/1

R0079
type: SequenceAnnotation
persistent identity: annotation1017909
display id: annotation1017909
version: 1
top level: las TetO
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I14015/annotation1017909/range1017909/1
type: Range
persistent identity: range1017909
display id: range1017909
version: 1
top level: las TetO
direction: direction/forward
start: 1
end: 151
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026120
display id: annotation1026120
version: 1
top level: las TetO
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I14015/annotation1026120/range1026120/1
type: Range
persistent identity: range1026120
display id: range1026120
version: 1
top level: las TetO
direction: direction/forward
start: 117
end: 122
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026116
display id: annotation1026116
version: 1
top level: las TetO
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I14015/annotation1026116/range1026116/1
type: Range
persistent identity: range1026116
display id: range1026116
version: 1
top level: las TetO
direction: direction/forward
start: 140
end: 145
orientation: inline

TetR
type: SequenceAnnotation
persistent identity: annotation1017910
display id: annotation1017910
version: 1
top level: las TetO
location: https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I14015/annotation1017910/range1017910/1
type: Range
persistent identity: range1017910
display id: range1017910
version: 1
top level: las TetO
direction: direction/forward
start: 152
end: 170
orientation: inline

BBa_K327006
type: ComponentDefinition
persistent identity: BBa K327006
display id: BBa_K327006
version: 1
was generated by: iGEM to SBOL conversion
description: Reworked light sensor
created: 2010-08-24T11:00:00Z
modified: 2015-05-08T01:12:03Z
mutable provenance: Registry
owned by: james
owned by: myers
mutable description: This part is very similar to BBa_M30109, but with the pTetR (R0040) promoter replaced with a constitutive promoter, to allow use of TetR elsewhere within the system.
discontinued: false
dominant: false
group u list: _447_
m user id: 0
owner id: 6138
owning group id: 415
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Keegan Owsley
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_I15010
component: BBa_B0034
component: BBa_J23119
component: BBa_I15009
component: BBa_I15008
component: BBa_I13453
sequence annotation: BBa_I13453
sequence annotation: BBa_B0034
sequence annotation: BBa_I15008
sequence annotation: BBa_I15009
sequence annotation: BBa_B0015
sequence annotation: BBa_J23119
sequence annotation: BBa_I15010
sequence: https://synbiohub.org/public/igem/BBa_K327006_sequence/1

BBa_B0015
type: Component
persistent identity: component2298049
display id: component2298049
version: 1
top level: BBa_K327006
definition: BBa_B0015
access: public

BBa_I15010
type: Component
persistent identity: component2298042
display id: component2298042
version: 1
top level: BBa_K327006
definition: BBa_I15010
access: public

BBa_B0034
type: Component
persistent identity: component2298039
display id: component2298039
version: 1
top level: BBa_K327006
definition: BBa_B0034
access: public

BBa_J23119
type: Component
persistent identity: component2298037
display id: component2298037
version: 1
top level: BBa_K327006
definition: BBa_J23119
access: public

BBa_B0015
type: Component
persistent identity: component2298036
display id: component2298036
version: 1
top level: BBa_K327006
definition: BBa_B0015
access: public

BBa_I15009
type: Component
persistent identity: component2298029
display id: component2298029
version: 1
top level: BBa_K327006
definition: BBa_I15009
access: public

BBa_B0034
type: Component
persistent identity: component2298026
display id: component2298026
version: 1
top level: BBa_K327006
definition: BBa_B0034
access: public

BBa_I15008
type: Component
persistent identity: component2298024
display id: component2298024
version: 1
top level: BBa_K327006
definition: BBa_I15008
access: public

BBa_B0034
type: Component
persistent identity: component2298022
display id: component2298022
version: 1
top level: BBa_K327006
definition: BBa_B0034
access: public

BBa_I13453
type: Component
persistent identity: component2298020
display id: component2298020
version: 1
top level: BBa_K327006
definition: BBa_I13453
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2298020
display id: annotation2298020
version: 1
top level: BBa_K327006
location: https://synbiohub.org/public/igem/BBa_K327006/annotation2298020/range2298020/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K327006/annotation2298020/range2298020/1
type: Range
persistent identity: range2298020
display id: range2298020
version: 1
top level: BBa_K327006
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2298022
display id: annotation2298022
version: 1
top level: BBa_K327006
location: https://synbiohub.org/public/igem/BBa_K327006/annotation2298022/range2298022/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K327006/annotation2298022/range2298022/1
type: Range
persistent identity: range2298022
display id: range2298022
version: 1
top level: BBa_K327006
direction: direction/reverse
start: 139
end: 150
orientation: inline

BBa_I15008
type: SequenceAnnotation
persistent identity: annotation2298024
display id: annotation2298024
version: 1
top level: BBa_K327006
location: https://synbiohub.org/public/igem/BBa_K327006/annotation2298024/range2298024/1
component: BBa_I15008

https://synbiohub.org/public/igem/BBa_K327006/annotation2298024/range2298024/1
type: Range
persistent identity: range2298024
display id: range2298024
version: 1
top level: BBa_K327006
direction: direction/reverse
start: 157
end: 907
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2298026
display id: annotation2298026
version: 1
top level: BBa_K327006
location: https://synbiohub.org/public/igem/BBa_K327006/annotation2298026/range2298026/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K327006/annotation2298026/range2298026/1
type: Range
persistent identity: range2298026
display id: range2298026
version: 1
top level: BBa_K327006
direction: direction/reverse
start: 916
end: 927
orientation: inline

BBa_I15009
type: SequenceAnnotation
persistent identity: annotation2298029
display id: annotation2298029
version: 1
top level: BBa_K327006
location: https://synbiohub.org/public/igem/BBa_K327006/annotation2298029/range2298029/1
component: BBa_I15009

https://synbiohub.org/public/igem/BBa_K327006/annotation2298029/range2298029/1
type: Range
persistent identity: range2298029
display id: range2298029
version: 1
top level: BBa_K327006
direction: direction/reverse
start: 934
end: 1708
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2298036
display id: annotation2298036
version: 1
top level: BBa_K327006
location: https://synbiohub.org/public/igem/BBa_K327006/annotation2298036/range2298036/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K327006/annotation2298036/range2298036/1
type: Range
persistent identity: range2298036
display id: range2298036
version: 1
top level: BBa_K327006
direction: direction/reverse
start: 1717
end: 1845
orientation: inline

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2298037
display id: annotation2298037
version: 1
top level: BBa_K327006
location: https://synbiohub.org/public/igem/BBa_K327006/annotation2298037/range2298037/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K327006/annotation2298037/range2298037/1
type: Range
persistent identity: range2298037
display id: range2298037
version: 1
top level: BBa_K327006
direction: direction/reverse
start: 1854
end: 1888
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2298039
display id: annotation2298039
version: 1
top level: BBa_K327006
location: https://synbiohub.org/public/igem/BBa_K327006/annotation2298039/range2298039/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K327006/annotation2298039/range2298039/1
type: Range
persistent identity: range2298039
display id: range2298039
version: 1
top level: BBa_K327006
direction: direction/reverse
start: 1897
end: 1908
orientation: inline

BBa_I15010
type: SequenceAnnotation
persistent identity: annotation2298042
display id: annotation2298042
version: 1
top level: BBa_K327006
location: https://synbiohub.org/public/igem/BBa_K327006/annotation2298042/range2298042/1
component: BBa_I15010

https://synbiohub.org/public/igem/BBa_K327006/annotation2298042/range2298042/1
type: Range
persistent identity: range2298042
display id: range2298042
version: 1
top level: BBa_K327006
direction: direction/reverse
start: 1915
end: 4177
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2298049
display id: annotation2298049
version: 1
top level: BBa_K327006
location: https://synbiohub.org/public/igem/BBa_K327006/annotation2298049/range2298049/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K327006/annotation2298049/range2298049/1
type: Range
persistent identity: range2298049
display id: range2298049
version: 1
top level: BBa_K327006
direction: direction/reverse
start: 4186
end: 4314
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: iGEM to SBOL conversion
description: heme oxygenase (ho1) from Synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
top level: BBa_I15008
direction: direction/forward
start: 727
end: 751
orientation: inline

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: iGEM to SBOL conversion
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Second of two required phycocyanobilin biosynthetic genes.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: PcyA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
top level: BBa_I15009
direction: direction/forward
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
top level: BBa_I15009
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_I15010
type: ComponentDefinition
persistent identity: BBa I15010
display id: BBa_I15010
version: 1
was generated by: iGEM to SBOL conversion
description: cph8 (Cph1/EnvZ fusion)
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
mutable description: Chimeric Cph1 light receptor/EnvZ protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Silent mutation at base 108 (G-A) to remove PstI site
star: false
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cph8
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15010_sequence/1

Cph8
type: SequenceAnnotation
persistent identity: annotation1891613
display id: annotation1891613
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
type: Range
persistent identity: range1891613
display id: range1891613
version: 1
top level: BBa_I15010
direction: direction/forward
start: 1
end: 2238
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214031
display id: annotation2214031
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
type: Range
persistent identity: range2214031
display id: range2214031
version: 1
top level: BBa_I15010
direction: direction/forward
start: 2239
end: 2263
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

https://synbiohub.org/public/igem/BBa_K327014_sequence/1
type: Sequence
persistent identity: BBa K327014 sequence
display id: BBa_K327014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagaggcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacactccctatcagtgatagagatactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14015_sequence/1
type: Sequence
persistent identity: BBa I14015 sequence
display id: BBa_I14015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcccctcgctgagcgcgtcccggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatcaaggctacctgccagttctggcaggtttggccgcgggttctttttggtacactccctatcagtgatagaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K327006_sequence/1
type: Sequence
persistent identity: BBa K327006 sequence
display id: BBa_K327006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagcccca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15008_sequence/1
type: Sequence
persistent identity: BBa I15008 sequence
display id: BBa_I15008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15009_sequence/1
type: Sequence
persistent identity: BBa I15009 sequence
display id: BBa_I15009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15010_sequence/1
type: Sequence
persistent identity: BBa I15010 sequence
display id: BBa_I15010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z