##gff-version 3
##sequence-region BBa_K327015 1 2875
BBa_K327015	.	ribosome_entry_site	246	257	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K327015	.	conserved_region	250	253	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327015	.	ribosome_entry_site	2036	2047	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K327015	.	conserved_region	2040	2043	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327015	.	engineered_region	1	237	.	+	0	ID=BBa_I746351;Name=BBa_I746351
BBa_K327015	.	engineered_region	1	12	.	+	0	ID=annotation1943868;Name=B0034;Parent=BBa_I746351
BBa_K327015	.	start_codon	19	19	.	+	0	ID=annotation1943869;Name=PSP3 pag;Parent=BBa_I746351
BBa_K327015	.	CDS	19	237	.	+	0	ID=annotation1943870;Name=PSP3 pag;Parent=BBa_I746351
BBa_K327015	.	promoter	939	1035	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_K327015	.	non_covalent_binding_site	995	1011	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K327015	.	non_covalent_binding_site	1019	1035	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K327015	.	promoter	985	990	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K327015	.	engineered_region	939	1035	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K327015	.	promoter	1009	1014	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K327015	.	promoter	996	996	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K327015	.	non_covalent_binding_site	944	963	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K327015	.	promoter	1032	1035	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K327015	.	CDS	264	905	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_K327015	.	CDS	264	866	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K327015	.	engineered_tag	906	930	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K327015	.	tag	867	899	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K327015	.	ribosome_entry_site	1044	1055	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K327015	.	conserved_region	1048	1051	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327015	.	terminator	2835	2875	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K327015	.	polyA_site	2862	2875	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K327015	.	stem_loop	2842	2861	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K327015	.	stop_codon	2868	2868	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K327015	.	engineered_region	2835	2875	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K327015	.	CDS	1062	1730	.	+	0	ID=BBa_C0052;Name=BBa_C0052
BBa_K327015	.	CDS	1062	1730	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_K327015	.	tag	1692	1730	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_K327015	.	engineered_region	1062	1730	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_K327015	.	engineered_tag	1731	1755	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_K327015	.	CDS	2054	2713	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K327015	.	engineered_tag	2714	2738	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K327015	.	CDS	2057	2673	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K327015	.	tag	2674	2707	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K327015	.	terminator	2747	2826	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K327015	.	stem_loop	2758	2801	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K327015	.	engineered_region	2747	2826	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K327015	.	engineered_region	1764	2027	.	+	0	ID=BBa_I746352;Name=BBa_I746352
BBa_K327015	.	CDS	1785	2027	.	+	0	ID=annotation1943872;Name=phiR73 delta;Parent=BBa_I746352
BBa_K327015	.	start_codon	1785	1785	.	+	0	ID=annotation1943873;Name=phiR73 delta;Parent=BBa_I746352
BBa_K327015	.	sequence_alteration	1865	1865	.	+	0	ID=annotation1943874;Name=silent mutation to remove PstI site;Parent=BBa_I746352
BBa_K327015	.	engineered_region	1764	1775	.	+	0	ID=annotation1943871;Name=B0034;Parent=BBa_I746352
>BBa_K327015
aaagaggagaaatactagatgatgcactgcccgttatgccaaaacgctgcacatgctcgcactagccggtaccttagca
ccgaaacgaaagaacgttatcaccagtgccaaaacataaattgcggatgtacatttatcacttttgagacactatcaag
attcattgtgaaaccggggactgttgatcctgctccgccccaccccatcagaaaccaacaacagcaactttggctttga
tactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtga
aatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgac
ggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgta
tcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctcccc
gcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaa
gctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaa
aaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctct
gcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgct
gctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagtaagcacctg
taggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctgg
cggtgatatactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttgga
cttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagc
gaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaa
tgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaa
gcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggc
tgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactgg
acgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcata
gatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcg
gtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgata
gtgctagtgtagatctctactagagaaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcat
acccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacat
ttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcacc
ggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagaaagaggagaaatactaga
tgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaac
ccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgac
gccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttac
gtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctac
agaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatat
gcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggg
aaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagc
cttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgac
gaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata