BBa_K327015
type: ComponentDefinition
persistent identity: BBa K327015
display id: BBa_K327015
version: 1
was generated by: iGEM to SBOL conversion
description: Lux activated, C1lam repressed switch
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:03Z
mutable provenance: registry
owned by: james
owned by: myers
mutable description: A switch which is activated by lux and repressed by C1lam
discontinued: true
dominant: false
experience: experience/None
group u list: _447_
m user id: 0
owner id: 6137
owning group id: 415
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: na
star: false
creator: Jennifer Lau
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0012
component: BBa_B0010
component: BBa_I746351
component: BBa_B0034
component: BBa_R0065
component: BBa_C0078
component: BBa_C0040
component: BBa_I746352
component: BBa_C0052
sequence annotation: BBa_I746351
sequence annotation: BBa_B0034
sequence annotation: BBa_C0078
sequence annotation: BBa_R0065
sequence annotation: BBa_C0052
sequence annotation: BBa_I746352
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K327015_sequence/1

BBa_B0012
type: Component
persistent identity: component2085397
display id: component2085397
version: 1
top level: BBa_K327015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2085395
display id: component2085395
version: 1
top level: BBa_K327015
definition: BBa_B0010
access: public

BBa_I746351
type: Component
persistent identity: component2085363
display id: component2085363
version: 1
top level: BBa_K327015
definition: BBa_I746351
access: public

BBa_B0034
type: Component
persistent identity: component2085379
display id: component2085379
version: 1
top level: BBa_K327015
definition: BBa_B0034
access: public

BBa_R0065
type: Component
persistent identity: component2085370
display id: component2085370
version: 1
top level: BBa_K327015
definition: cI+luxR
access: public

BBa_C0078
type: Component
persistent identity: component2085368
display id: component2085368
version: 1
top level: BBa_K327015
definition: lasI
access: public

BBa_B0034
type: Component
persistent identity: component2085365
display id: component2085365
version: 1
top level: BBa_K327015
definition: BBa_B0034
access: public

BBa_C0040
type: Component
persistent identity: component2085393
display id: component2085393
version: 1
top level: BBa_K327015
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component2085390
display id: component2085390
version: 1
top level: BBa_K327015
definition: BBa_B0034
access: public

BBa_I746352
type: Component
persistent identity: component2085388
display id: component2085388
version: 1
top level: BBa_K327015
definition: BBa_I746352
access: public

BBa_C0052
type: Component
persistent identity: component2085381
display id: component2085381
version: 1
top level: BBa_K327015
definition: cI 434
access: public

BBa_I746351
type: SequenceAnnotation
persistent identity: annotation2085363
display id: annotation2085363
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085363/range2085363/1
component: BBa_I746351

https://synbiohub.org/public/igem/BBa_K327015/annotation2085363/range2085363/1
type: Range
persistent identity: range2085363
display id: range2085363
version: 1
top level: BBa_K327015
direction: direction/reverse
start: 1
end: 237
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085365
display id: annotation2085365
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085365/range2085365/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K327015/annotation2085365/range2085365/1
type: Range
persistent identity: range2085365
display id: range2085365
version: 1
top level: BBa_K327015
direction: direction/reverse
start: 246
end: 257
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2085368
display id: annotation2085368
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085368/range2085368/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K327015/annotation2085368/range2085368/1
type: Range
persistent identity: range2085368
display id: range2085368
version: 1
top level: BBa_K327015
direction: direction/reverse
start: 264
end: 905
orientation: inline

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation2085370
display id: annotation2085370
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085370/range2085370/1
component: BBa_R0065

https://synbiohub.org/public/igem/BBa_K327015/annotation2085370/range2085370/1
type: Range
persistent identity: range2085370
display id: range2085370
version: 1
top level: BBa_K327015
direction: direction/forward
start: 939
end: 1035
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085379
display id: annotation2085379
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085379/range2085379/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K327015/annotation2085379/range2085379/1
type: Range
persistent identity: range2085379
display id: range2085379
version: 1
top level: BBa_K327015
direction: direction/reverse
start: 1044
end: 1055
orientation: inline

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation2085381
display id: annotation2085381
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085381/range2085381/1
component: BBa_C0052

https://synbiohub.org/public/igem/BBa_K327015/annotation2085381/range2085381/1
type: Range
persistent identity: range2085381
display id: range2085381
version: 1
top level: BBa_K327015
direction: direction/forward
start: 1062
end: 1730
orientation: inline

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2085388
display id: annotation2085388
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085388/range2085388/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_K327015/annotation2085388/range2085388/1
type: Range
persistent identity: range2085388
display id: range2085388
version: 1
top level: BBa_K327015
direction: direction/reverse
start: 1764
end: 2027
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2085390
display id: annotation2085390
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085390/range2085390/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K327015/annotation2085390/range2085390/1
type: Range
persistent identity: range2085390
display id: range2085390
version: 1
top level: BBa_K327015
direction: direction/reverse
start: 2036
end: 2047
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2085393
display id: annotation2085393
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085393/range2085393/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K327015/annotation2085393/range2085393/1
type: Range
persistent identity: range2085393
display id: range2085393
version: 1
top level: BBa_K327015
direction: direction/reverse
start: 2054
end: 2713
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2085395
display id: annotation2085395
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085395/range2085395/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K327015/annotation2085395/range2085395/1
type: Range
persistent identity: range2085395
display id: range2085395
version: 1
top level: BBa_K327015
direction: direction/forward
start: 2747
end: 2826
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2085397
display id: annotation2085397
version: 1
top level: BBa_K327015
location: https://synbiohub.org/public/igem/BBa_K327015/annotation2085397/range2085397/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K327015/annotation2085397/range2085397/1
type: Range
persistent identity: range2085397
display id: range2085397
version: 1
top level: BBa_K327015
direction: direction/forward
start: 2835
end: 2875
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

lasI
type: ComponentDefinition
persistent identity: BBa C0078
display id: BBa_C0078
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for lasI protein, which produces the chemical signal AI-1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: LasI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0078_sequence/1

LasI
type: SequenceAnnotation
persistent identity: annotation305970
display id: annotation305970
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
type: Range
persistent identity: range305970
display id: range305970
version: 1
top level: lasI
direction: direction/forward
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306608
display id: annotation306608
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
type: Range
persistent identity: range306608
display id: range306608
version: 1
top level: lasI
direction: direction/forward
start: 604
end: 636
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214003
display id: annotation2214003
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
type: Range
persistent identity: range2214003
display id: range2214003
version: 1
top level: lasI
direction: direction/forward
start: 643
end: 667
orientation: inline

cI 434
type: ComponentDefinition
persistent identity: BBa C0052
display id: BBa_C0052
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage 434 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage 434
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence contains a LVA tag for faster degredation and has no RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984) References (unparsed) here: Nikolnikov,S., Posfai,G. and Sain,B. The construction of a versatile plasmid vector that allows direct selection of fragments cloned into six unique sites of the cI gene of coliphage 434. Gene 30 (1-3), 261-265 (1984)
star: true
creator: Maia Mahoney
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0052
sequence annotation: cI 434
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0052_sequence/1

BBa_C0052
type: SequenceAnnotation
persistent identity: annotation7035
display id: annotation7035
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0052/annotation7035/range7035/1
type: Range
persistent identity: range7035
display id: range7035
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

cI 434
type: SequenceAnnotation
persistent identity: annotation1743
display id: annotation1743
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0052/annotation1743/range1743/1
type: Range
persistent identity: range1743
display id: range1743
version: 1
top level: cI 434
direction: direction/forward
start: 1
end: 669
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1745
display id: annotation1745
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0052/annotation1745/range1745/1
type: Range
persistent identity: range1745
display id: range1745
version: 1
top level: cI 434
direction: direction/forward
start: 631
end: 669
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213992
display id: annotation2213992
version: 1
top level: cI 434
location: https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0052/annotation2213992/range2213992/1
type: Range
persistent identity: range2213992
display id: range2213992
version: 1
top level: cI 434
direction: direction/forward
start: 670
end: 694
orientation: inline

cI+luxR
type: ComponentDefinition
persistent identity: BBa R0065
display id: BBa_R0065
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lambda cI and luxR regulated -- hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR. LuxI cI activity of promoter + + zero + - maximum - + zero - - leaky (no quantitative information)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight <genbank>AF170104</genbank>.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0065
sequence annotation: Lux Box
sequence annotation: -10
sequence annotation: OR2 cI
sequence annotation: lux p(R) start
sequence annotation: -35
sequence annotation: OR1 cI
sequence: https://synbiohub.org/public/igem/BBa_R0065_sequence/1

BBa_R0065
type: SequenceAnnotation
persistent identity: annotation1986774
display id: annotation1986774
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0065/annotation1986774/range1986774/1
type: Range
persistent identity: range1986774
display id: range1986774
version: 1
top level: cI+luxR
direction: direction/forward
start: 1
end: 97
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation1986775
display id: annotation1986775
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0065/annotation1986775/range1986775/1
type: Range
persistent identity: range1986775
display id: range1986775
version: 1
top level: cI+luxR
direction: direction/forward
start: 6
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986776
display id: annotation1986776
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0065/annotation1986776/range1986776/1
type: Range
persistent identity: range1986776
display id: range1986776
version: 1
top level: cI+luxR
direction: direction/forward
start: 47
end: 52
orientation: inline

OR2 cI
type: SequenceAnnotation
persistent identity: annotation1986777
display id: annotation1986777
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0065/annotation1986777/range1986777/1
type: Range
persistent identity: range1986777
display id: range1986777
version: 1
top level: cI+luxR
direction: direction/forward
start: 57
end: 73
orientation: inline

lux p(R) start
type: SequenceAnnotation
persistent identity: annotation1986778
display id: annotation1986778
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0065/annotation1986778/range1986778/1
type: Range
persistent identity: range1986778
display id: range1986778
version: 1
top level: cI+luxR
direction: direction/forward
start: 58
end: 58
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986779
display id: annotation1986779
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0065/annotation1986779/range1986779/1
type: Range
persistent identity: range1986779
display id: range1986779
version: 1
top level: cI+luxR
direction: direction/forward
start: 71
end: 76
orientation: inline

OR1 cI
type: SequenceAnnotation
persistent identity: annotation1986780
display id: annotation1986780
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0065/annotation1986780/range1986780/1
type: Range
persistent identity: range1986780
display id: range1986780
version: 1
top level: cI+luxR
direction: direction/forward
start: 81
end: 97
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986781
display id: annotation1986781
version: 1
top level: cI+luxR
location: https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0065/annotation1986781/range1986781/1
type: Range
persistent identity: range1986781
display id: range1986781
version: 1
top level: cI+luxR
direction: direction/forward
start: 94
end: 97
orientation: inline

BBa_I746351
type: ComponentDefinition
persistent identity: BBa I746351
display id: BBa_I746351
version: 1
was generated by: iGEM to SBOL conversion
description: pag activator from PSP3 phage
created: 2007-09-11T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
mutable description: The pag activator taken from PSP3 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The part does contain a RBS (B0034) already.
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: PSP3 pag
sequence: https://synbiohub.org/public/igem/BBa_I746351_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1943868
display id: annotation1943868
version: 1
top level: BBa_I746351
location: https://synbiohub.org/public/igem/BBa_I746351/annotation1943868/range1943868/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I746351/annotation1943868/range1943868/1
type: Range
persistent identity: range1943868
display id: range1943868
version: 1
top level: BBa_I746351
direction: direction/forward
start: 1
end: 12
orientation: inline

PSP3 pag
type: SequenceAnnotation
persistent identity: annotation1943870
display id: annotation1943870
version: 1
top level: BBa_I746351
location: https://synbiohub.org/public/igem/BBa_I746351/annotation1943870/range1943870/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746351/annotation1943870/range1943870/1
type: Range
persistent identity: range1943870
display id: range1943870
version: 1
top level: BBa_I746351
direction: direction/forward
start: 19
end: 237
orientation: inline

PSP3 pag
type: SequenceAnnotation
persistent identity: annotation1943869
display id: annotation1943869
version: 1
top level: BBa_I746351
location: https://synbiohub.org/public/igem/BBa_I746351/annotation1943869/range1943869/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746351/annotation1943869/range1943869/1
type: Range
persistent identity: range1943869
display id: range1943869
version: 1
top level: BBa_I746351
direction: direction/forward
start: 19
end: 19
orientation: inline

BBa_I746352
type: ComponentDefinition
persistent identity: BBa I746352
display id: BBa_I746352
version: 1
was generated by: iGEM to SBOL conversion
description: delta activator from phiR73 phage
created: 2007-09-11T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
mutable description: The delta activator taken from phiR73 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The part does contain a RBS (B0034) already.
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: phiR73 delta
sequence annotation: silent mutation to remove PstI site
sequence: https://synbiohub.org/public/igem/BBa_I746352_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1943871
display id: annotation1943871
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
type: Range
persistent identity: range1943871
display id: range1943871
version: 1
top level: BBa_I746352
direction: direction/forward
start: 1
end: 12
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943872
display id: annotation1943872
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
type: Range
persistent identity: range1943872
display id: range1943872
version: 1
top level: BBa_I746352
direction: direction/forward
start: 22
end: 264
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943873
display id: annotation1943873
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
type: Range
persistent identity: range1943873
display id: range1943873
version: 1
top level: BBa_I746352
direction: direction/forward
start: 22
end: 22
orientation: inline

silent mutation to remove PstI site
type: SequenceAnnotation
persistent identity: annotation1943874
display id: annotation1943874
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
type: Range
persistent identity: range1943874
display id: range1943874
version: 1
top level: BBa_I746352
direction: direction/forward
start: 102
end: 102
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K327015_sequence/1
type: Sequence
persistent identity: BBa K327015 sequence
display id: BBa_K327015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatgcactgcccgttatgccaaaacgctgcacatgctcgcactagccggtaccttagcaccgaaacgaaagaacgttatcaccagtgccaaaacataaattgcggatgtacatttatcacttttgagacactatcaagattcattgtgaaaccggggactgttgatcctgctccgccccaccccatcagaaaccaacaacagcaactttggctttgatactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0078_sequence/1
type: Sequence
persistent identity: BBa C0078 sequence
display id: BBa_C0078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0052_sequence/1
type: Sequence
persistent identity: BBa C0052 sequence
display id: BBa_C0052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0065_sequence/1
type: Sequence
persistent identity: BBa R0065 sequence
display id: BBa_R0065_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746351_sequence/1
type: Sequence
persistent identity: BBa I746351 sequence
display id: BBa_I746351_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatgcactgcccgttatgccaaaacgctgcacatgctcgcactagccggtaccttagcaccgaaacgaaagaacgttatcaccagtgccaaaacataaattgcggatgtacatttatcacttttgagacactatcaagattcattgtgaaaccggggactgttgatcctgctccgccccaccccatcagaaaccaacaacagcaactttggctttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746352_sequence/1
type: Sequence
persistent identity: BBa I746352 sequence
display id: BBa_I746352_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z