LOCUS BBa_K327016 2875 bp DNA linear 31-Oct-2024 DEFINITION Lux activated, C1lam repressed switch ACCESSION BBa_K327016 VERSION BBa_K327016.1 FEATURES Location/Qualifiers misc_feature 1..12 /label=B0034 misc_feature 19..19 /label=PSP3 pag CDS 19..237 /label=PSP3 pag misc_feature 1..237 /label=BBa_I746351 misc_feature 250..253 /label=conserved RBS 246..257 /label=BBa_B0034 CDS 264..866 /label=LasI misc_feature 867..899 /label=LVA misc_feature 906..930 /label=Help:Barcodes CDS 264..905 /label=BBa_C0078 misc_feature 939..1035 /label=BBa_R0065 misc_feature 944..963 /label=Lux Box promoter 985..990 /label=-10 misc_feature 995..1011 /label=OR2 cI promoter 996..996 /label=lux p(R) start promoter 1009..1014 /label=-35 misc_feature 1019..1035 /label=OR1 cI promoter 1032..1035 /label=-10 promoter 939..1035 /label=BBa_R0065 misc_feature 1048..1051 /label=conserved RBS 1044..1055 /label=BBa_B0034 CDS 1062..1730 /label=cI 434 misc_feature 1692..1730 /label=LVA misc_feature 1062..1730 /label=BBa_C0052 misc_feature 1731..1755 /label=Help:Barcodes CDS 1062..1730 /label=BBa_C0052 misc_feature 1764..1775 /label=B0034 CDS 1785..2027 /label=phiR73 delta misc_feature 1785..1785 /label=phiR73 delta misc_feature 1865..1865 /label=silent mutation to remove PstI site misc_feature 1764..2027 /label=BBa_I746352 misc_feature 2040..2043 /label=conserved RBS 2036..2047 /label=BBa_B0034 CDS 2057..2673 /label=tetR misc_feature 2674..2707 /label=SsrA misc_feature 2714..2738 /label=Help:Barcodes CDS 2054..2713 /label=BBa_C0040 stem_loop 2758..2801 /label=stem_loop misc_feature 2747..2826 /label=BBa_B0010 terminator 2747..2826 /label=BBa_B0010 stem_loop 2842..2861 /label=T7 TE misc_feature 2868..2868 /label=stop polyA_site 2862..2875 /label=polya misc_feature 2835..2875 /label=BBa_B0012 terminator 2835..2875 /label=BBa_B0012 ORIGIN 1 aaagaggaga aatactagat gatgcactgc ccgttatgcc aaaacgctgc acatgctcgc 61 actagccggt accttagcac cgaaacgaaa gaacgttatc accagtgcca aaacataaat 121 tgcggatgta catttatcac ttttgagaca ctatcaagat tcattgtgaa accggggact 181 gttgatcctg ctccgcccca ccccatcaga aaccaacaac agcaactttg gctttgatac 241 tagagaaaga ggagaaatac tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca 301 aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt 361 gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg tccccgtact 421 acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg cgtatcttcg 481 acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag 541 ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag 601 gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct cgttactcct 661 tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga 721 tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg 781 ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac ggtggtgttc 841 tggttgaaca gcgtctggct gtttccgctg ctaacgacga aaactacgct ctggttgctt 901 aataactctg atagtgctag tgtagatctc tactagagta agcacctgta ggatcgtaca 961 ggtttacgca agaaaatggt ttgttatagt cgaataacac cgtgcgtgtt gactatttta 1021 cctctggcgg tgatatacta gagaaagagg agaaatacta gatgagtatt tcttccaggg 1081 taaaaagcaa aagaatccag cttggactta accaggctga acttgctcaa aaggtgggga 1141 ctacccagca gtctatagag cagctcgaaa acggtaaaac taagcgacca cgctttttac 1201 cagaacttgc gtcagctctt ggcgtaagtg ttgactggct gctcaatggc acctctgatt 1261 cgaatgttag atttgttggg cacgttgagc ccaaagggaa atatccattg attagcatgg 1321 ttagagctgg ttcgtggtgt gaagcttgtg aaccctacga tatcaaggac attgatgaat 1381 ggtatgacag tgacgttaac ttattaggca atggattctg gctgaaggtt gaaggtgatt 1441 ccatgacctc acctgtaggt caaagcatcc ctgaaggtca tatggtgtta gtagatactg 1501 gacgggagcc agtgaatgga agccttgttg tagccaaact gactgacgcg aacgaagcaa 1561 cattcaagaa actggtcata gatggcggtc agaagtacct gaaaggcctg aatccttcat 1621 ggcctatgac tcctatcaac ggaaactgca agattatcgg tgttgtcgtg gaagcgaggg 1681 taaaattcgt agctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt 1741 gctagtgtag atctctacta gagaaagagg agaaatacta gatgatgcgc tgccctttct 1801 gtcgtcattc agcgcatacc cgcaccagcc ggtatgtgag tgacaatgtc aaagaaagtt 1861 atctccagtg ccagaatatt tactgttcgg cgacatttaa aacgcatgag tcaatttgtg 1921 ccgtgattcg ttctccggtc acggaggaaa aaccagcacc ggcaagcaca gcaccggctg 1981 ttgtccgaaa agttaaaggc tgttacagct caccattcaa ccattaatac tagagaaaga 2041 ggagaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct 2101 gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt 2161 agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc 2221 cattgagatg ttagataggc accatactca cttttgccct ttagaagggg aaagctggca 2281 agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg 2341 agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca 2401 attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc 2461 tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga 2521 agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt 2581 atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg 2641 attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt 2701 agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata 2761 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2821 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //