LOCUS       BBa_K327017             2730 bp    DNA     linear       25-Nov-2024
DEFINITION  lux OR delta cI activated "off" part
ACCESSION   BBa_K327017
VERSION     BBa_K327017.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=PLL
     promoter        1..92
                     /label=BBa_I746365
     misc_feature    105..108
                     /label=conserved
     RBS             101..112
                     /label=BBa_B0034
     CDS             119..721
                     /label=LasI
     misc_feature    722..754
                     /label=LVA
     misc_feature    761..785
                     /label=Help:Barcodes
     CDS             119..785
                     /label=BBa_C0078
     misc_feature    794..890
                     /label=BBa_R0065
     misc_feature    799..818
                     /label=Lux Box
     promoter        840..845
                     /label=-10
     misc_feature    850..866
                     /label=OR2 cI
     promoter        851..851
                     /label=lux p(R) start
     promoter        864..869
                     /label=-35
     misc_feature    874..890
                     /label=OR1 cI
     promoter        887..890
                     /label=-10
     promoter        794..890
                     /label=BBa_R0065
     misc_feature    903..906
                     /label=conserved
     RBS             899..910
                     /label=BBa_B0034
     CDS             917..1585
                     /label=cI 434
     misc_feature    1547..1585
                     /label=LVA
     misc_feature    917..1585
                     /label=BBa_C0052
     misc_feature    1586..1610
                     /label=Help:Barcodes
     CDS             917..1585
                     /label=BBa_C0052
     misc_feature    1619..1630
                     /label=B0034
     CDS             1640..1882
                     /label=phiR73 delta
     misc_feature    1640..1640
                     /label=phiR73 delta
     misc_feature    1720..1720
                     /label=silent mutation to remove PstI site
     misc_feature    1619..1882
                     /label=BBa_I746352
     misc_feature    1895..1898
                     /label=conserved
     RBS             1891..1902
                     /label=BBa_B0034
     CDS             1912..2528
                     /label=tetR
     misc_feature    2529..2562
                     /label=SsrA
     misc_feature    2569..2593
                     /label=Help:Barcodes
     CDS             1909..2593
                     /label=BBa_C0040
     stem_loop       2613..2656
                     /label=stem_loop
     misc_feature    2602..2681
                     /label=BBa_B0010
     terminator      2602..2681
                     /label=BBa_B0010
     stem_loop       2697..2716
                     /label=T7 TE
     misc_feature    2723..2723
                     /label=stop
     polyA_site      2717..2730
                     /label=polya
     misc_feature    2690..2730
                     /label=BBa_B0012
     terminator      2690..2730
                     /label=BBa_B0012
     terminator      2602..2730
                     /label=BBa_B0015
ORIGIN
        1 gtggagatga acaaaaaaca cacagccatt gtaagacagc ctgaacaaat cccccctgtt
       61 gcgtctgctg aaaatattca caaaataaag cgtactagag aaagaggaga aatactagat
      121 gatcgttcag atcggtcgtc gtgaagagtt cgacaaaaaa ctgctgggtg aaatgcacaa
      181 actgcgtgct caggttttca aagaacgtaa aggttgggac gtttccgtta tcgacgaaat
      241 ggaaatcgac ggttacgacg ctctgtcccc gtactacatg ctgatccagg aagacacccc
      301 ggaagctcag gttttcggtt gctggcgtat cttcgacacc accggtccgt acatgctgaa
      361 aaacaccttc ccggaactgc tgcacggtaa agaagctccg tgctccccgc acatctggga
      421 actgtcccgt ttcgctatca actccggtca gaaaggttcc ctgggtttct ccgactgcac
      481 cctggaagct atgcgtgctc tggctcgtta ctccttgcag aacgacatcc agaccctggt
      541 taccgttacc accgttggtg ttgaaaaaat gatgatccgt gctggtctgg acgtttcccg
      601 tttcggtccg cacctgaaaa tcggtatcga acgtgctgtt gctctgcgta tcgaactgaa
      661 cgctaaaacc cagatcgctc tgtacggtgg tgttctggtt gaacagcgtc tggctgtttc
      721 cgctgctaac gacgaaaact acgctctggt tgcttaataa ctctgatagt gctagtgtag
      781 atctctacta gagtaagcac ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt
      841 atagtcgaat aacaccgtgc gtgttgacta ttttacctct ggcggtgata tactagagaa
      901 agaggagaaa tactagatga gtatttcttc cagggtaaaa agcaaaagaa tccagcttgg
      961 acttaaccag gctgaacttg ctcaaaaggt ggggactacc cagcagtcta tagagcagct
     1021 cgaaaacggt aaaactaagc gaccacgctt tttaccagaa cttgcgtcag ctcttggcgt
     1081 aagtgttgac tggctgctca atggcacctc tgattcgaat gttagatttg ttgggcacgt
     1141 tgagcccaaa gggaaatatc cattgattag catggttaga gctggttcgt ggtgtgaagc
     1201 ttgtgaaccc tacgatatca aggacattga tgaatggtat gacagtgacg ttaacttatt
     1261 aggcaatgga ttctggctga aggttgaagg tgattccatg acctcacctg taggtcaaag
     1321 catccctgaa ggtcatatgg tgttagtaga tactggacgg gagccagtga atggaagcct
     1381 tgttgtagcc aaactgactg acgcgaacga agcaacattc aagaaactgg tcatagatgg
     1441 cggtcagaag tacctgaaag gcctgaatcc ttcatggcct atgactccta tcaacggaaa
     1501 ctgcaagatt atcggtgttg tcgtggaagc gagggtaaaa ttcgtagctg caaacgacga
     1561 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagaa
     1621 agaggagaaa tactagatga tgcgctgccc tttctgtcgt cattcagcgc atacccgcac
     1681 cagccggtat gtgagtgaca atgtcaaaga aagttatctc cagtgccaga atatttactg
     1741 ttcggcgaca tttaaaacgc atgagtcaat ttgtgccgtg attcgttctc cggtcacgga
     1801 ggaaaaacca gcaccggcaa gcacagcacc ggctgttgtc cgaaaagtta aaggctgtta
     1861 cagctcacca ttcaaccatt aatactagag aaagaggaga aatactagat gtccagatta
     1921 gataaaagta aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt
     1981 ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat
     2041 gtaaaaaata agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat
     2101 actcactttt gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa
     2161 agttttagat gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg
     2221 cctacagaaa aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt
     2281 ttttcactag agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc
     2341 gtattggaag atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat
     2401 agtatgccgc cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca
     2461 gccttcttat tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa
     2521 agtgggtccg ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc
     2581 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2641 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     2701 caccttcggg tgggcctttc tgcgtttata
//