##gff-version 3
##sequence-region BBa_K327017 1 2730
BBa_K327017	.	engineered_region	1619	1882	.	+	0	ID=BBa_I746352;Name=BBa_I746352
BBa_K327017	.	engineered_region	1619	1630	.	+	0	ID=annotation1943871;Name=B0034;Parent=BBa_I746352
BBa_K327017	.	start_codon	1640	1640	.	+	0	ID=annotation1943873;Name=phiR73 delta;Parent=BBa_I746352
BBa_K327017	.	CDS	1640	1882	.	+	0	ID=annotation1943872;Name=phiR73 delta;Parent=BBa_I746352
BBa_K327017	.	sequence_alteration	1720	1720	.	+	0	ID=annotation1943874;Name=silent mutation to remove PstI site;Parent=BBa_I746352
BBa_K327017	.	CDS	119	785	.	+	0	ID=BBa_C0078;Name=BBa_C0078
BBa_K327017	.	CDS	119	721	.	+	0	ID=annotation305970;Name=LasI;Parent=BBa_C0078
BBa_K327017	.	engineered_tag	761	785	.	+	0	ID=annotation2214003;Name=Help:Barcodes;Parent=BBa_C0078
BBa_K327017	.	tag	722	754	.	+	0	ID=annotation306608;Name=LVA;Parent=BBa_C0078
BBa_K327017	.	terminator	2602	2730	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K327017	.	terminator	2690	2730	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K327017	.	stem_loop	2697	2716	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K327017	.	engineered_region	2690	2730	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K327017	.	stop_codon	2723	2723	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K327017	.	polyA_site	2717	2730	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K327017	.	terminator	2602	2681	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K327017	.	engineered_region	2602	2681	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K327017	.	stem_loop	2613	2656	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K327017	.	CDS	917	1585	.	+	0	ID=BBa_C0052;Name=BBa_C0052
BBa_K327017	.	engineered_region	917	1585	.	+	0	ID=annotation7035;Name=BBa_C0052;Parent=BBa_C0052
BBa_K327017	.	CDS	917	1585	.	+	0	ID=annotation1743;Name=cI 434;Parent=BBa_C0052
BBa_K327017	.	tag	1547	1585	.	+	0	ID=annotation1745;Name=LVA;Parent=BBa_C0052
BBa_K327017	.	engineered_tag	1586	1610	.	+	0	ID=annotation2213992;Name=Help:Barcodes;Parent=BBa_C0052
BBa_K327017	.	ribosome_entry_site	1891	1902	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K327017	.	conserved_region	1895	1898	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327017	.	ribosome_entry_site	899	910	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K327017	.	conserved_region	903	906	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327017	.	promoter	1	92	.	+	0	ID=BBa_I746365;Name=BBa_I746365
BBa_K327017	.	promoter	1	92	.	+	0	ID=annotation1943788;Name=PLL;Parent=BBa_I746365
BBa_K327017	.	ribosome_entry_site	101	112	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K327017	.	conserved_region	105	108	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K327017	.	promoter	794	890	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_K327017	.	promoter	840	845	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K327017	.	non_covalent_binding_site	799	818	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K327017	.	non_covalent_binding_site	850	866	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K327017	.	engineered_region	794	890	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K327017	.	promoter	887	890	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K327017	.	non_covalent_binding_site	874	890	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K327017	.	promoter	864	869	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K327017	.	promoter	851	851	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K327017	.	CDS	1909	2593	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K327017	.	CDS	1912	2528	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K327017	.	engineered_tag	2569	2593	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K327017	.	tag	2529	2562	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
>BBa_K327017
gtggagatgaacaaaaaacacacagccattgtaagacagcctgaacaaatcccccctgttgcgtctgctgaaaatattc
acaaaataaagcgtactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaa
aactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacga
aatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttc
ggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaag
ctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccga
ctgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccacc
gttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaac
gtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtct
ggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctacta
gagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgact
attttacctctggcggtgatatactagagaaagaggagaaatactagatgagtatttcttccagggtaaaaagcaaaag
aatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaac
ggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggca
cctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctgg
ttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggc
aatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgt
tagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaa
gaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaac
tgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagctt
aataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgatgcgctgccctttctgtcg
tcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttac
tgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccgg
caagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagaaagag
gagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcg
aaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcg
ggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctgg
caagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttag
gtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactaga
gaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgct
aaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaag
gtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtc
cgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata