BBa_K329008
1
BBa_K329008
Double terminator (B0015) - attC - mRFP
2010-10-11T11:00:00Z
2015-05-08T01:12:03Z
This construct was originally integrated in a pSUlib vector (low copy plasmid, Kanamycin resistant) according to the biobrick standard.
This part is composed of a double terminator (<partinfo>BBa_B0015</partinfo>) followed by an attC site and mRFP (with an RBS). When the IntI integrase is expressed, it can specifically excise a region of DNA flanked by attC sites.
<br>Important note : RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix.
false
false
_449_
0
7402
9
It's complicated
false
We used BioBrick Standard Assembly.
false
Raphael Pantier
BBa_K329008_sequence
1
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagggttataacaattcattcaagccgacgccgcttcgcggcgcggcttaattcaagcgttataacctactagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaagcggccgctgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z