BBa_K329009
1
BBa_K329009
attC - double terminator (B0015) - attC - mRFP
2010-10-11T11:00:00Z
2015-05-08T01:12:03Z
<partinfo>BBa_K329008</partinfo>
This part is composed of a double terminator (<partinfo>BBa_B0015</partinfo>) flanked by attC sites and followed by mRFP (with an RBS). When the IntI integrase is expressed (and if there is a promoter upstream of this part), it can specifically excise the terminator and triggers the expression of mRFP.
<br>Important note : RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix.
false
false
_449_
0
7402
9
It's complicated
false
RBS-mRFP is situated between the SpeI and PstI restriction sites, the excision of this gene permit to restore a normal biobrick suffix. We used BioBrick Standard Assembly.
false
Raphael Pantier
BBa_K329009_sequence
1
agggttataacaattcattcaagccgacgccgcttcgcggcgcggcttaattcaagcgttataacctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagggttataacaattcattcaagccgacgccgcttcgcggcgcggcttaattcaagcgttataacctactagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaagcggccgctgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z