BBa_K329010
1
BBa_K329010
RBS + Int HK022
2010-10-16T11:00:00Z
2015-05-08T01:12:03Z
Parts come from a PCR amplification on a genomic source.
This integrase comes from the double-stranded HK022 DNA enterobacteriophage of Escherichia coli, member of the lambdoid or lambda-like group of phages.
This protein is necessary for both the integration and the excision of the phage DNA into the host cell.
The integration process only requires Int to be expressed, and recombines the attP site from the phage with the attB attachment site on the bacterial chromosome.
false
false
_449_
0
5926
9
It's complicated
false
The original RBS was kept, superposed with the end of the Xis gene.
false
Xavier Duportet
annotation2087842
1
Int HK022
range2087842
1
20
1074
annotation2087841
1
RBS
range2087841
1
7
12
BBa_K329010_sequence
1
atgggaagaaggcgaagtcatgagcgccgggatttaccccctaacctttatataagaaacaatggatattactgctacagggacccaaggacgggtaaagagtttggattaggccgagacaggcgaatcgcaatcactgaagctatacaggccaacattgagttgctatccgggaacaggcgtgagtcactgatagacagaattaaaggcgctgacgcaatcactcttcatgcgtggcttgaccgatatgaaacaatcctcagcgagaggggtatcaggccgaaaactctactcgactacgccagcaaaatcagagcaatccgaagaaaattgccggacaaaccgctcgctgacatatcaacgaaagaggtggcagcaatgctaaacacctacgtagcagaaggcaaatcggcttccgcaaaattgatcaggtcaacccttgttgacgtttttcgtgaggcaatagccgaggggcatgtggctacgaatccggtaacagcaacccgcacagcaaaatcagaagtaaggcgttcaagactgacagctaatgaatatgtcgcgatataccatgcagccgaaccgctcccaatctggctgagactggcaatggatttggctgtcgttacagggcagagagtaggcgatttgtgcagaatgaaatggtcagacataaacgacaaccatcttcacattgaacagagtaaaacaggggctaaactcgccattccactaacgctaacgattgacgcgctcaatatctcattggctgatacactacagcaatgcagggaggccagcagcagtgaaactataatcgcatcaaagcatcacgatccgctttccccgaaaacagtatcaaagtattttacaaaggcgagaaatgcatctggactctcatttgatggaaacccgccaacattccatgaactgcgtagcctgtcagcgaggctataccggaaccagattggcgataagtttgctcaacgtcttctcgggcataaatcagattcaatggcggcgcggtatagggacagccgtggacgggaatgggacaaaattgaaatcgacaaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z