BBa_K329011 1 BBa_K329011 RBS + Xis-Int HK022 2010-10-16T11:00:00Z 2015-05-08T01:12:03Z PCR amplification on a genomic source. This part allows the expression of both the Excisionase and the Integrase of the double-stranded HK022 DNA enterobacteriophage of Escherichia coli, member of the lambdoid or lambda-like group of phages. Expression of these two proteins promotes the excision of the phage DNA by recombining the attR and the attL sites. After excision, the original attB and attP sites are reformed. false false _449_ 0 5926 9 It's complicated false Kept the original RBS and the structural organisation of these two genes (the beginning of Int overlaps with the end of Xis). false Xavier Duportet annotation2113822 1 Xis range2113822 1 20 238 annotation2113823 1 Int range2113823 1 216 1289 annotation2087858 1 RBS Xis range2087858 1 7 12 BBa_K329011_sequence 1 attctgcggagactttgcgatgtacttaacacttcaggagtggaacgctcgccagcgacgcccaagaagccttgaaacagttcgtcgatgggtgcgcgaatgcaggatattccctcctccggttaaggatggaagagagtatctgttccacgaatcagcggtaaaggttgacttaaatcgaccagtaacaggtagccttttgaagaggatcagaaatgggaagaaggcgaagtcatgagcgccgggatttaccccctaacctttatataagaaacaatggatattactgctacagggacccaaggacgggtaaagagtttggattaggccgagacaggcgaatcgcaatcactgaagctatacaggccaacattgagttgctatccgggaacaggcgtgagtcactgatagacagaattaaaggcgctgacgcaatcactcttcatgcgtggcttgaccgatatgaaacaatcctcagcgagaggggtatcaggccgaaaactctactcgactacgccagcaaaatcagagcaatccgaagaaaattgccggacaaaccgctcgctgacatatcaacgaaagaggtggcagcaatgctaaacacctacgtagcagaaggcaaatcggcttccgcaaaattgatcaggtcaacccttgttgacgtttttcgtgaggcaatagccgaggggcatgtggctacgaatccggtaacagcaacccgcacagcaaaatcagaagtaaggcgttcaagactgacagctaatgaatatgtcgcgatataccatgcagccgaaccgctcccaatctggctgagactggcaatggatttggctgtcgttacagggcagagagtaggcgatttgtgcagaatgaaatggtcagacataaacgacaaccatcttcacattgaacagagtaaaacaggggctaaactcgccattccactaacgctaacgattgacgcgctcaatatctcattggctgatacactacagcaatgcagggaggccagcagcagtgaaactataatcgcatcaaagcatcacgatccgctttccccgaaaacagtatcaaagtattttacaaaggcgagaaatgcatctggactctcatttgatggaaacccgccaacattccatgaactgcgtagcctgtcagcgaggctataccggaaccagattggcgataagtttgctcaacgtcttctcgggcataaatcagattcaatggcggcgcggtatagggacagccgtggacgggaatgggacaaaattgaaatcgacaaatgataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z