LOCUS       BBa_K329051             1544 bp    DNA     linear       25-Nov-2024
DEFINITION  [KanR + LacZ] cassette
ACCESSION   BBa_K329051
VERSION     BBa_K329051.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..295
                     /label=lacZ
     misc_feature    62..64
                     /label=START
     misc_feature    290..295
                     /label=STOP
     CDS             62..295
                     /label=BBa_I732006
     stem_loop       315..358
                     /label=stem_loop
     misc_feature    304..383
                     /label=BBa_B0010
     terminator      304..383
                     /label=BBa_B0010
     stem_loop       399..418
                     /label=T7 TE
     misc_feature    425..425
                     /label=stop
     polyA_site      419..432
                     /label=polya
     misc_feature    392..432
                     /label=BBa_B0012
     terminator      392..432
                     /label=BBa_B0012
     terminator      304..432
                     /label=BBa_B0015
     CDS             589..1407
                     /label=kanamycin resistance
     misc_feature    441..1407
                     /label=BBa_P1003
     stem_loop       1427..1470
                     /label=stem_loop
     misc_feature    1416..1495
                     /label=BBa_B0010
     terminator      1416..1495
                     /label=BBa_B0010
     stem_loop       1511..1530
                     /label=T7 TE
     misc_feature    1537..1537
                     /label=stop
     polyA_site      1531..1544
                     /label=polya
     misc_feature    1504..1544
                     /label=BBa_B0012
     terminator      1504..1544
                     /label=BBa_B0012
     terminator      1416..1544
                     /label=BBa_B0015
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      121 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      181 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      241 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt aataatacta
      301 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      361 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      421 tctgcgttta tatactagag ctgatccttc aactcagcaa aagttcgatt tattcaacaa
      481 agccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
      541 tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt
      601 caacgggaaa cgtcttgctc ccgtccgcgc ttaaactcca acatggacgc tgatttatat
      661 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
      721 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
      781 gttacagatg agatggtccg tctcaactgg ctgacggagt ttatgcctct cccgaccatc
      841 aagcatttta tccgtactcc tgatgatgcg tggttactca ccaccgcgat tcctgggaaa
      901 acagccttcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
      961 gccgtgttcc tgcgccggtt acattcgatt cctgtttgta attgtccttt taacagcgat
     1021 cgtgtatttc gtcttgctca ggcgcaatca cgcatgaata acggtttggt tgatgcgagt
     1081 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcacaag
     1141 ctcttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     1201 atttttgacg aggggaaatt aataggttgt attgatgttg gacgggtcgg aatcgcagac
     1261 cgttaccagg accttgccat tctttggaac tgcctcggtg agttttctcc ttcattacag
     1321 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     1381 ttgatgctcg atgagttttt ctaataatac tagagccagg catcaaataa aacgaaaggc
     1441 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1501 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//