##gff-version 3
##sequence-region BBa_K329051 1 1544
BBa_K329051	.	promoter	1	35	.	+	0	ID=BBa_J23110;Name=BBa_J23110
BBa_K329051	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K329051	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K329051	.	terminator	1416	1544	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K329051	.	terminator	1416	1495	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K329051	.	engineered_region	1416	1495	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K329051	.	stem_loop	1427	1470	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K329051	.	terminator	1504	1544	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K329051	.	polyA_site	1531	1544	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K329051	.	stem_loop	1511	1530	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K329051	.	engineered_region	1504	1544	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K329051	.	stop_codon	1537	1537	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K329051	.	engineered_region	441	1407	.	+	0	ID=BBa_P1003;Name=BBa_P1003
BBa_K329051	.	CDS	589	1407	.	+	0	ID=annotation1897112;Name=kanamycin resistance;Parent=BBa_P1003
BBa_K329051	.	CDS	62	295	.	+	0	ID=BBa_I732006;Name=BBa_I732006
BBa_K329051	.	CDS	62	295	.	+	0	ID=annotation1936904;Name=lacZ;Parent=BBa_I732006
BBa_K329051	.	stop_codon	290	295	.	+	0	ID=annotation1936906;Name=STOP;Parent=BBa_I732006
BBa_K329051	.	start_codon	62	64	.	+	0	ID=annotation1936905;Name=START;Parent=BBa_I732006
BBa_K329051	.	terminator	304	432	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K329051	.	terminator	304	383	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K329051	.	engineered_region	304	383	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K329051	.	stem_loop	315	358	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K329051	.	terminator	392	432	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K329051	.	polyA_site	419	432	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K329051	.	stem_loop	399	418	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K329051	.	engineered_region	392	432	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K329051	.	stop_codon	425	425	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K329051
tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaccatgattacggat
tcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatcccc
ctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatg
gcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagctgatccttcaactcagcaaaagttcgatttatt
caacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaac
tgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgctt
aaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctat
cgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatg
agatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgc
gtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatatt
gttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtat
ttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctg
gcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttc
tcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgtt
accaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatgg
tattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataatactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttata