LOCUS       BBa_K329056             2169 bp    DNA     linear       22-Nov-2024
DEFINITION  [KanR + LacZ] in between WT_MUT[HK022] Tn916 Arms
ACCESSION   BBa_K329056
VERSION     BBa_K329056.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    118..307
                     /label=BBa_K329041
     misc_feature    44..117
                     /label=half of attR HK022
     misc_feature    44..313
                     /label=BBa_K329044
     promoter        322..356
                     /label=BBa_J23110
     misc_feature    369..372
                     /label=conserved
     RBS             365..376
                     /label=BBa_B0034
     CDS             383..616
                     /label=lacZ
     misc_feature    383..385
                     /label=START
     misc_feature    611..616
                     /label=STOP
     CDS             383..616
                     /label=BBa_I732006
     stem_loop       636..679
                     /label=stem_loop
     misc_feature    625..704
                     /label=BBa_B0010
     terminator      625..704
                     /label=BBa_B0010
     stem_loop       720..739
                     /label=T7 TE
     misc_feature    746..746
                     /label=stop
     polyA_site      740..753
                     /label=polya
     misc_feature    713..753
                     /label=BBa_B0012
     terminator      713..753
                     /label=BBa_B0012
     terminator      625..753
                     /label=BBa_B0015
     CDS             910..1728
                     /label=kanamycin resistance
     misc_feature    762..1728
                     /label=BBa_P1003
     stem_loop       1748..1791
                     /label=stem_loop
     misc_feature    1737..1816
                     /label=BBa_B0010
     terminator      1737..1816
                     /label=BBa_B0010
     stem_loop       1832..1851
                     /label=T7 TE
     misc_feature    1858..1858
                     /label=stop
     polyA_site      1852..1865
                     /label=polya
     misc_feature    1825..1865
                     /label=BBa_B0012
     terminator      1825..1865
                     /label=BBa_B0012
     terminator      1737..1865
                     /label=BBa_B0015
     misc_feature    322..1865
                     /label=BBa_K329051
     misc_feature    1874..2123
                     /label=BBa_K329046
     misc_feature    2124..2129
                     /label=Coupling
     misc_feature    2130..2135
                     /label=Mutation
     misc_feature    2136..2169
                     /label=Half of attL HK022 MUT
     misc_feature    1874..2169
                     /label=BBa_K329049
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagctagaga caaatgattt
       61 tattttgact aataatgacc tacttacatt aatttactga taaccttttt tattttaaaa
      121 atagcataaa aatctagtta tccgcataaa aactggactt atcacacttt atcaaggtca
      181 aaaccactca atttactact aatttactac ttatgaatga gctttgatac gacgatttat
      241 ccttgaaaag tgaagatata aagatacttc caataaaatt tgaatattta ataggtagac
      301 acttcaatac tagtactaga gtttacggct agctcagtcc taggtacaat gctagctact
      361 agagaaagag gagaaatact agatgaccat gattacggat tcactggccg tcgttttaca
      421 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      481 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      541 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
      601 ctggctggag taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      661 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      721 gctcaccttc gggtgggcct ttctgcgttt atatactaga gctgatcctt caactcagca
      781 aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca
      841 caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca
      901 aggggtgtta tgagccatat tcaacgggaa acgtcttgct cccgtccgcg cttaaactcc
      961 aacatggacg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     1021 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     1081 aaaggtagcg ttgccaatga tgttacagat gagatggtcc gtctcaactg gctgacggag
     1141 tttatgcctc tcccgaccat caagcatttt atccgtactc ctgatgatgc gtggttactc
     1201 accaccgcga ttcctgggaa aacagccttc caggtattag aagaatatcc tgattcaggt
     1261 gaaaatattg ttgatgcgct ggccgtgttc ctgcgccggt tacattcgat tcctgtttgt
     1321 aattgtcctt ttaacagcga tcgtgtattt cgtcttgctc aggcgcaatc acgcatgaat
     1381 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     1441 gtctggaaag aaatgcacaa gctcttgcca ttctcaccgg attcagtcgt cactcatggt
     1501 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     1561 ggacgggtcg gaatcgcaga ccgttaccag gaccttgcca ttctttggaa ctgcctcggt
     1621 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     1681 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaataata ctagagccag
     1741 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1801 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1861 ttatatacta gagtgcaaca ttcgattctg caatggcaga aatgaaacgc ttgaataaag
     1921 agaagcaaca ggagcgtctt gttgcttagt agtacaaatg aatttactac ttatttacca
     1981 cttctgacag ctaagacatg aggaaatatg caaagaaacg tgaagtatct tcctacagta
     2041 aaaatactcg aaagcacata gaataaggct ttacgagcat ttaagaaaat ataaaaagat
     2101 aattagaaat ttatactttg tttattttaa aaatccaact tattcaccta aagtgcaccg
     2161 accgtgaat
//