##gff-version 3
##sequence-region BBa_K329056 1 2169
BBa_K329056	.	engineered_region	322	1865	.	+	0	ID=BBa_K329051;Name=BBa_K329051
BBa_K329056	.	ribosome_entry_site	365	376	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K329051
BBa_K329056	.	conserved_region	369	372	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K329056	.	terminator	625	753	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K329051
BBa_K329056	.	terminator	625	704	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K329056	.	stem_loop	636	679	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K329056	.	engineered_region	625	704	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K329056	.	terminator	713	753	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K329056	.	engineered_region	713	753	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K329056	.	stop_codon	746	746	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K329056	.	polyA_site	740	753	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K329056	.	stem_loop	720	739	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K329056	.	engineered_region	762	1728	.	+	0	ID=BBa_P1003;Name=BBa_P1003;Parent=BBa_K329051
BBa_K329056	.	CDS	910	1728	.	+	0	ID=annotation1897112;Name=kanamycin resistance;Parent=BBa_P1003
BBa_K329056	.	promoter	322	356	.	+	0	ID=BBa_J23110;Name=BBa_J23110;Parent=BBa_K329051
BBa_K329056	.	CDS	383	616	.	+	0	ID=BBa_I732006;Name=BBa_I732006;Parent=BBa_K329051
BBa_K329056	.	stop_codon	611	616	.	+	0	ID=annotation1936906;Name=STOP;Parent=BBa_I732006
BBa_K329056	.	start_codon	383	385	.	+	0	ID=annotation1936905;Name=START;Parent=BBa_I732006
BBa_K329056	.	CDS	383	616	.	+	0	ID=annotation1936904;Name=lacZ;Parent=BBa_I732006
BBa_K329056	.	terminator	1737	1865	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K329051
BBa_K329056	.	terminator	1737	1816	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K329056	.	stem_loop	1748	1791	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K329056	.	engineered_region	1737	1816	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K329056	.	terminator	1825	1865	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K329056	.	engineered_region	1825	1865	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K329056	.	stop_codon	1858	1858	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K329056	.	polyA_site	1852	1865	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K329056	.	stem_loop	1832	1851	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K329056	.	sequence_feature	44	313	.	+	0	ID=BBa_K329044;Name=BBa_K329044
BBa_K329056	.	sequence_feature	44	117	.	+	0	ID=annotation2114008;Name=half of attR HK022;Parent=BBa_K329044
BBa_K329056	.	engineered_region	118	307	.	+	0	ID=annotation2113984;Name=BBa_K329041;Parent=BBa_K329044
BBa_K329056	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119
BBa_K329056	.	sequence_feature	1874	2169	.	+	0	ID=BBa_K329049;Name=BBa_K329049
BBa_K329056	.	sequence_feature	2136	2169	.	+	0	ID=annotation2113969;Name=Half of attL HK022 MUT;Parent=BBa_K329049
BBa_K329056	.	sequence_feature	2130	2135	.	+	0	ID=annotation2113968;Name=Mutation;Parent=BBa_K329049
BBa_K329056	.	engineered_region	1874	2123	.	+	0	ID=annotation2113966;Name=BBa_K329046;Parent=BBa_K329049
BBa_K329056	.	sequence_feature	2124	2129	.	+	0	ID=annotation2113967;Name=Coupling;Parent=BBa_K329049
>BBa_K329056
ttgacagctagctcagtcctaggtataatgctagctactagagctagagacaaatgattttattttgactaataatgac
ctacttacattaatttactgataaccttttttattttaaaaatagcataaaaatctagttatccgcataaaaactggac
ttatcacactttatcaaggtcaaaaccactcaatttactactaatttactacttatgaatgagctttgatacgacgatt
tatccttgaaaagtgaagatataaagatacttccaataaaatttgaatatttaataggtagacacttcaatactagtac
tagagtttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaccatgatta
cggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcaca
tccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggc
gaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctgatccttcaactcagcaaaagttcgat
ttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaat
aaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccg
cgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaa
tctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttac
agatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgat
gatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaa
atattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcg
tgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaat
ggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtg
atttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcaga
ccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaa
tatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtgcaacattcgattctgcaatgg
cagaaatgaaacgcttgaataaagagaagcaacaggagcgtcttgttgcttagtagtacaaatgaatttactacttatt
taccacttctgacagctaagacatgaggaaatatgcaaagaaacgtgaagtatcttcctacagtaaaaatactcgaaag
cacatagaataaggctttacgagcatttaagaaaatataaaaagataattagaaatttatactttgtttattttaaaaa
tccaacttattcacctaaagtgcaccgaccgtgaat