BBa_K329056
type: ComponentDefinition
persistent identity: BBa K329056
display id: BBa_K329056
version: 1
was generated by: iGEM to SBOL conversion
description: [KanR + LacZ] in between WT_MUT[HK022] Tn916 Arms
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:04Z
mutable provenance: to write
owned by: james
owned by: myers
mutable description: to write
discontinued: false
dominant: false
experience: experience/None
group u list: _449_
m user id: 0
owner id: 5926
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: to write
star: false
creator: Xavier Duportet
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K329049
component: BBa_K329051
component: BBa_K329044
component: BBa_J23119
sequence annotation: BBa_J23119
sequence annotation: BBa_K329044
sequence annotation: BBa_K329051
sequence annotation: BBa_K329049
sequence: https://synbiohub.org/public/igem/BBa_K329056_sequence/1

BBa_K329049
type: Component
persistent identity: component2114414
display id: component2114414
version: 1
top level: BBa_K329056
definition: BBa_K329049
access: public

BBa_K329051
type: Component
persistent identity: component2114409
display id: component2114409
version: 1
top level: BBa_K329056
definition: BBa_K329051
access: public

BBa_K329044
type: Component
persistent identity: component2114385
display id: component2114385
version: 1
top level: BBa_K329056
definition: BBa_K329044
access: public

BBa_J23119
type: Component
persistent identity: component2114382
display id: component2114382
version: 1
top level: BBa_K329056
definition: BBa_J23119
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2114382
display id: annotation2114382
version: 1
top level: BBa_K329056
location: https://synbiohub.org/public/igem/BBa_K329056/annotation2114382/range2114382/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K329056/annotation2114382/range2114382/1
type: Range
persistent identity: range2114382
display id: range2114382
version: 1
top level: BBa_K329056
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K329044
type: SequenceAnnotation
persistent identity: annotation2114385
display id: annotation2114385
version: 1
top level: BBa_K329056
location: https://synbiohub.org/public/igem/BBa_K329056/annotation2114385/range2114385/1
component: BBa_K329044

https://synbiohub.org/public/igem/BBa_K329056/annotation2114385/range2114385/1
type: Range
persistent identity: range2114385
display id: range2114385
version: 1
top level: BBa_K329056
direction: direction/reverse
start: 44
end: 313
orientation: inline

BBa_K329051
type: SequenceAnnotation
persistent identity: annotation2114409
display id: annotation2114409
version: 1
top level: BBa_K329056
location: https://synbiohub.org/public/igem/BBa_K329056/annotation2114409/range2114409/1
component: BBa_K329051

https://synbiohub.org/public/igem/BBa_K329056/annotation2114409/range2114409/1
type: Range
persistent identity: range2114409
display id: range2114409
version: 1
top level: BBa_K329056
direction: direction/reverse
start: 322
end: 1865
orientation: inline

BBa_K329049
type: SequenceAnnotation
persistent identity: annotation2114414
display id: annotation2114414
version: 1
top level: BBa_K329056
location: https://synbiohub.org/public/igem/BBa_K329056/annotation2114414/range2114414/1
component: BBa_K329049

https://synbiohub.org/public/igem/BBa_K329056/annotation2114414/range2114414/1
type: Range
persistent identity: range2114414
display id: range2114414
version: 1
top level: BBa_K329056
direction: direction/reverse
start: 1874
end: 2169
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_K329044
type: ComponentDefinition
persistent identity: BBa K329044
display id: BBa_K329044
version: 1
was generated by: iGEM to SBOL conversion
description: Right arm Tn916 - Wild Type + Mutant HK022
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:04Z
mutable provenance: to write
owned by: james
owned by: myers
mutable description: to write
discontinued: false
dominant: false
experience: experience/None
group u list: _449_
m user id: 0
owner id: 5926
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: to write
star: false
creator: Xavier Duportet
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: half of attR HK022
sequence annotation: BBa_K329041
sequence: https://synbiohub.org/public/igem/BBa_K329044_sequence/1

half of attR HK022
type: SequenceAnnotation
persistent identity: annotation2114008
display id: annotation2114008
version: 1
top level: BBa_K329044
location: https://synbiohub.org/public/igem/BBa_K329044/annotation2114008/range2114008/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K329044/annotation2114008/range2114008/1
type: Range
persistent identity: range2114008
display id: range2114008
version: 1
top level: BBa_K329044
direction: direction/forward
start: 1
end: 74
orientation: inline

BBa_K329041
type: SequenceAnnotation
persistent identity: annotation2113984
display id: annotation2113984
version: 1
top level: BBa_K329044
location: https://synbiohub.org/public/igem/BBa_K329044/annotation2113984/range2113984/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K329044/annotation2113984/range2113984/1
type: Range
persistent identity: range2113984
display id: range2113984
version: 1
top level: BBa_K329044
direction: direction/forward
start: 75
end: 264
orientation: inline

BBa_K329049
type: ComponentDefinition
persistent identity: BBa K329049
display id: BBa_K329049
version: 1
was generated by: iGEM to SBOL conversion
description: Left arm Tn916 - Wild Type + Mutant HK022
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:04Z
mutable provenance: to write
owned by: james
owned by: myers
mutable description: to write
discontinued: false
dominant: false
experience: experience/None
group u list: _449_
m user id: 0
owner id: 5926
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: to write
star: false
creator: Xavier Duportet
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: BBa_K329046
sequence annotation: Coupling
sequence annotation: Mutation
sequence annotation: Half of attL HK022 MUT
sequence: https://synbiohub.org/public/igem/BBa_K329049_sequence/1

BBa_K329046
type: SequenceAnnotation
persistent identity: annotation2113966
display id: annotation2113966
version: 1
top level: BBa_K329049
location: https://synbiohub.org/public/igem/BBa_K329049/annotation2113966/range2113966/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K329049/annotation2113966/range2113966/1
type: Range
persistent identity: range2113966
display id: range2113966
version: 1
top level: BBa_K329049
direction: direction/forward
start: 1
end: 250
orientation: inline

Coupling
type: SequenceAnnotation
persistent identity: annotation2113967
display id: annotation2113967
version: 1
top level: BBa_K329049
location: https://synbiohub.org/public/igem/BBa_K329049/annotation2113967/range2113967/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K329049/annotation2113967/range2113967/1
type: Range
persistent identity: range2113967
display id: range2113967
version: 1
top level: BBa_K329049
direction: direction/forward
start: 251
end: 256
orientation: inline

Mutation
type: SequenceAnnotation
persistent identity: annotation2113968
display id: annotation2113968
version: 1
top level: BBa_K329049
location: https://synbiohub.org/public/igem/BBa_K329049/annotation2113968/range2113968/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K329049/annotation2113968/range2113968/1
type: Range
persistent identity: range2113968
display id: range2113968
version: 1
top level: BBa_K329049
direction: direction/forward
start: 257
end: 262
orientation: inline

Half of attL HK022 MUT
type: SequenceAnnotation
persistent identity: annotation2113969
display id: annotation2113969
version: 1
top level: BBa_K329049
location: https://synbiohub.org/public/igem/BBa_K329049/annotation2113969/range2113969/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K329049/annotation2113969/range2113969/1
type: Range
persistent identity: range2113969
display id: range2113969
version: 1
top level: BBa_K329049
direction: direction/forward
start: 263
end: 296
orientation: inline

BBa_K329051
type: ComponentDefinition
persistent identity: BBa K329051
display id: BBa_K329051
version: 1
was generated by: iGEM to SBOL conversion
description: [KanR + LacZ] cassette
created: 2010-10-24T11:00:00Z
modified: 2015-05-08T01:12:04Z
mutable provenance: to write
owned by: james
owned by: myers
mutable description: to write
discontinued: false
dominant: false
experience: experience/None
group u list: _449_
m user id: 0
owner id: 5926
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: to write
star: false
creator: Xavier Duportet
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0015
component: BBa_P1003
component: BBa_I732006
component: BBa_B0034
component: BBa_J23110
sequence annotation: BBa_J23110
sequence annotation: BBa_B0034
sequence annotation: BBa_I732006
sequence annotation: BBa_B0015
sequence annotation: BBa_P1003
sequence: https://synbiohub.org/public/igem/BBa_K329051_sequence/1

BBa_B0015
type: Component
persistent identity: component2114007
display id: component2114007
version: 1
top level: BBa_K329051
definition: BBa_B0015
access: public

BBa_P1003
type: Component
persistent identity: component2114000
display id: component2114000
version: 1
top level: BBa_K329051
definition: kanR
access: public

BBa_B0015
type: Component
persistent identity: component2113998
display id: component2113998
version: 1
top level: BBa_K329051
definition: BBa_B0015
access: public

BBa_I732006
type: Component
persistent identity: component2113991
display id: component2113991
version: 1
top level: BBa_K329051
definition: lacZ-alpha
access: public

BBa_B0034
type: Component
persistent identity: component2113987
display id: component2113987
version: 1
top level: BBa_K329051
definition: BBa_B0034
access: public

BBa_J23110
type: Component
persistent identity: component2113985
display id: component2113985
version: 1
top level: BBa_K329051
definition: BBa_J23110
access: public

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2113985
display id: annotation2113985
version: 1
top level: BBa_K329051
location: https://synbiohub.org/public/igem/BBa_K329051/annotation2113985/range2113985/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K329051/annotation2113985/range2113985/1
type: Range
persistent identity: range2113985
display id: range2113985
version: 1
top level: BBa_K329051
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2113987
display id: annotation2113987
version: 1
top level: BBa_K329051
location: https://synbiohub.org/public/igem/BBa_K329051/annotation2113987/range2113987/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K329051/annotation2113987/range2113987/1
type: Range
persistent identity: range2113987
display id: range2113987
version: 1
top level: BBa_K329051
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation2113991
display id: annotation2113991
version: 1
top level: BBa_K329051
location: https://synbiohub.org/public/igem/BBa_K329051/annotation2113991/range2113991/1
component: BBa_I732006

https://synbiohub.org/public/igem/BBa_K329051/annotation2113991/range2113991/1
type: Range
persistent identity: range2113991
display id: range2113991
version: 1
top level: BBa_K329051
direction: direction/reverse
start: 62
end: 295
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2113998
display id: annotation2113998
version: 1
top level: BBa_K329051
location: https://synbiohub.org/public/igem/BBa_K329051/annotation2113998/range2113998/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K329051/annotation2113998/range2113998/1
type: Range
persistent identity: range2113998
display id: range2113998
version: 1
top level: BBa_K329051
direction: direction/reverse
start: 304
end: 432
orientation: inline

BBa_P1003
type: SequenceAnnotation
persistent identity: annotation2114000
display id: annotation2114000
version: 1
top level: BBa_K329051
location: https://synbiohub.org/public/igem/BBa_K329051/annotation2114000/range2114000/1
component: BBa_P1003

https://synbiohub.org/public/igem/BBa_K329051/annotation2114000/range2114000/1
type: Range
persistent identity: range2114000
display id: range2114000
version: 1
top level: BBa_K329051
direction: direction/reverse
start: 441
end: 1407
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2114007
display id: annotation2114007
version: 1
top level: BBa_K329051
location: https://synbiohub.org/public/igem/BBa_K329051/annotation2114007/range2114007/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K329051/annotation2114007/range2114007/1
type: Range
persistent identity: range2114007
display id: range2114007
version: 1
top level: BBa_K329051
direction: direction/reverse
start: 1416
end: 1544
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

lacZ-alpha
type: ComponentDefinition
persistent identity: BBa I732006
display id: BBa_I732006
version: 1
was generated by: iGEM to SBOL conversion
description: lacZ alpha fragment
created: 2007-07-06T11:00:00Z
modified: 2015-08-31T04:07:56Z
mutable provenance: PCR amplified from BBa_J33202 without the RBS.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
discontinued: false
dominant: true
experience: experience/Works
group u list: _156_
m user id: 0
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: None
star: true
creator: Zhan Jian
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: START
sequence annotation: lacZ
sequence annotation: STOP
sequence: https://synbiohub.org/public/igem/BBa_I732006_sequence/1

START
type: SequenceAnnotation
persistent identity: annotation1936905
display id: annotation1936905
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I732006/annotation1936905/range1936905/1
type: Range
persistent identity: range1936905
display id: range1936905
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 3
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation1936904
display id: annotation1936904
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I732006/annotation1936904/range1936904/1
type: Range
persistent identity: range1936904
display id: range1936904
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 1
end: 234
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation1936906
display id: annotation1936906
version: 1
top level: lacZ-alpha
location: https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I732006/annotation1936906/range1936906/1
type: Range
persistent identity: range1936906
display id: range1936906
version: 1
top level: lacZ-alpha
direction: direction/forward
start: 229
end: 234
orientation: inline

kanR
type: ComponentDefinition
persistent identity: BBa P1003
display id: BBa_P1003
version: 1
was generated by: iGEM to SBOL conversion
description: kanamycin resistance cassette
created: 2006-01-23T12:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
mutable description: Kanamycin resistance cassette for modular BioBricks vector construction.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: kanamycin resistance
sequence: https://synbiohub.org/public/igem/BBa_P1003_sequence/1

kanamycin resistance
type: SequenceAnnotation
persistent identity: annotation1897112
display id: annotation1897112
version: 1
top level: kanR
location: https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1003/annotation1897112/range1897112/1
type: Range
persistent identity: range1897112
display id: range1897112
version: 1
top level: kanR
direction: direction/forward
start: 149
end: 967
orientation: inline

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K329056_sequence/1
type: Sequence
persistent identity: BBa K329056 sequence
display id: BBa_K329056_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagctagagacaaatgattttattttgactaataatgacctacttacattaatttactgataaccttttttattttaaaaatagcataaaaatctagttatccgcataaaaactggacttatcacactttatcaaggtcaaaaccactcaatttactactaatttactacttatgaatgagctttgatacgacgatttatccttgaaaagtgaagatataaagatacttccaataaaatttgaatatttaataggtagacacttcaatactagtactagagtttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtgcaacattcgattctgcaatggcagaaatgaaacgcttgaataaagagaagcaacaggagcgtcttgttgcttagtagtacaaatgaatttactacttatttaccacttctgacagctaagacatg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K329044_sequence/1
type: Sequence
persistent identity: BBa K329044 sequence
display id: BBa_K329044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagagacaaatgattttattttgactaataatgacctacttacattaatttactgataaccttttttattttaaaaatagcataaaaatctagttatccgcataaaaactggacttatcacactttatcaaggtcaaaaccactcaatttactactaatttactacttatgaatgagctttgatacgacgatttatccttgaaaagtgaagatataaagatacttccaataaaatttgaatatttaataggtagacacttcaatactag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K329049_sequence/1
type: Sequence
persistent identity: BBa K329049 sequence
display id: BBa_K329049_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgcaacattcgattctgcaatggcagaaatgaaacgcttgaataaagagaagcaacaggagcgtcttgttgcttagtagtacaaatgaatttactacttatttaccacttctgacagctaagacatgaggaaatatgcaaagaaacgtgaagtatcttcctacagtaaaaatactcgaaagcacatagaataaggctttacgagcatttaagaaaatataaaaagataattagaaatttatactttgtttattttaaaaatccaacttattcacctaaagtgcaccgaccgtgaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K329051_sequence/1
type: Sequence
persistent identity: BBa K329051 sequence
display id: BBa_K329051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732006_sequence/1
type: Sequence
persistent identity: BBa I732006 sequence
display id: BBa_I732006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1003_sequence/1
type: Sequence
persistent identity: BBa P1003 sequence
display id: BBa_P1003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23110_sequence/1
type: Sequence
persistent identity: BBa J23110 sequence
display id: BBa_J23110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z