LOCUS       BBa_K329057             2235 bp    DNA     linear       23-Dec-2024
DEFINITION  [KanR + LacZ] in between MUT_MUT[HK022] Tn916 Arms
ACCESSION   BBa_K329057
VERSION     BBa_K329057.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    172..361
                     /label=mutated right arm of Tn916
     misc_feature    44..171
                     /label=mutated attR HK022
     misc_feature    44..367
                     /label=BBa_K329045
     promoter        376..410
                     /label=BBa_J23110
     misc_feature    423..426
                     /label=conserved
     RBS             419..430
                     /label=BBa_B0034
     CDS             437..670
                     /label=lacZ
     misc_feature    437..439
                     /label=START
     misc_feature    665..670
                     /label=STOP
     CDS             437..670
                     /label=BBa_I732006
     stem_loop       690..733
                     /label=stem_loop
     misc_feature    679..758
                     /label=BBa_B0010
     terminator      679..758
                     /label=BBa_B0010
     stem_loop       774..793
                     /label=T7 TE
     misc_feature    800..800
                     /label=stop
     polyA_site      794..807
                     /label=polya
     misc_feature    767..807
                     /label=BBa_B0012
     terminator      767..807
                     /label=BBa_B0012
     terminator      679..807
                     /label=BBa_B0015
     CDS             964..1782
                     /label=kanamycin resistance
     misc_feature    816..1782
                     /label=BBa_P1003
     stem_loop       1802..1845
                     /label=stem_loop
     misc_feature    1791..1870
                     /label=BBa_B0010
     terminator      1791..1870
                     /label=BBa_B0010
     stem_loop       1886..1905
                     /label=T7 TE
     misc_feature    1912..1912
                     /label=stop
     polyA_site      1906..1919
                     /label=polya
     misc_feature    1879..1919
                     /label=BBa_B0012
     terminator      1879..1919
                     /label=BBa_B0012
     terminator      1791..1919
                     /label=BBa_B0015
     misc_feature    376..1919
                     /label=BBa_K329051
     misc_feature    1928..2177
                     /label=Left arm HK022 MUT
     misc_feature    2178..2183
                     /label=Coupling
     misc_feature    2184..2189
                     /label=Mutation
     misc_feature    2190..2235
                     /label=Half of attL HK022 MUT
     misc_feature    1928..2235
                     /label=BBa_K329050
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagctagaga ggtcactaat
       61 accatctaag tagttgattc atagtgactg catatgttgt gttttacagt attatgtagt
      121 ctgtttttta tgcaaaatct aatttaatat attgatacct tttttatttt aaaaatagca
      181 taaaaatcta gttatccgca taaaaactgg acttatcaca ctttatcaag gtcaaaacca
      241 ctcaatttac tactaattta ctacttatga atgagctttg atacgacgat ttatccttga
      301 aaagtgaaga tataaagata cttccaataa aatttgaata tttaataggt agacacttca
      361 atactagtac tagagtttac ggctagctca gtcctaggta caatgctagc tactagagaa
      421 agaggagaaa tactagatga ccatgattac ggattcactg gccgtcgttt tacaacgtcg
      481 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      541 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      601 gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct
      661 ggagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      721 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      781 cttcgggtgg gcctttctgc gtttatatac tagagctgat ccttcaactc agcaaaagtt
      841 cgatttattc aacaaagcca cgttgtgtct caaaatctct gatgttacat tgcacaagat
      901 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
      961 gttatgagcc atattcaacg ggaaacgtct tgctcccgtc cgcgcttaaa ctccaacatg
     1021 gacgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1081 atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1141 agcgttgcca atgatgttac agatgagatg gtccgtctca actggctgac ggagtttatg
     1201 cctctcccga ccatcaagca ttttatccgt actcctgatg atgcgtggtt actcaccacc
     1261 gcgattcctg ggaaaacagc cttccaggta ttagaagaat atcctgattc aggtgaaaat
     1321 attgttgatg cgctggccgt gttcctgcgc cggttacatt cgattcctgt ttgtaattgt
     1381 ccttttaaca gcgatcgtgt atttcgtctt gctcaggcgc aatcacgcat gaataacggt
     1441 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1501 aaagaaatgc acaagctctt gccattctca ccggattcag tcgtcactca tggtgatttc
     1561 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacgg
     1621 gtcggaatcg cagaccgtta ccaggacctt gccattcttt ggaactgcct cggtgagttt
     1681 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     1741 aaattgcagt ttcatttgat gctcgatgag tttttctaat aatactagag ccaggcatca
     1801 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     1861 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     1921 actagagcta gagtgcaaca ttcgattctg caatggcaga aatgaaacgc ttgaataaag
     1981 agaagcaaca ggagcgtctt gttgcttagt agtacaaatg aatttactac ttatttacca
     2041 cttctgacag ctaagacatc cgttgaagcc tgctttttta tactaacttg agcgaaacga
     2101 aaaatactcg aaattctacc cgtagaatgc gtactggtaa tgcaaactaa ataaaaagat
     2161 aattagaaat ttatactttg tttattttaa aaatccaact tattcaccta aagtgcaccg
     2221 accgtgaatt actag
//