##gff-version 3
##sequence-region BBa_K329057 1 2235
BBa_K329057	.	engineered_region	376	1919	.	+	0	ID=BBa_K329051;Name=BBa_K329051
BBa_K329057	.	promoter	376	410	.	+	0	ID=BBa_J23110;Name=BBa_J23110;Parent=BBa_K329051
BBa_K329057	.	terminator	679	807	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K329051
BBa_K329057	.	terminator	679	758	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K329057	.	stem_loop	690	733	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K329057	.	engineered_region	679	758	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K329057	.	terminator	767	807	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K329057	.	engineered_region	767	807	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K329057	.	stop_codon	800	800	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K329057	.	polyA_site	794	807	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K329057	.	stem_loop	774	793	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K329057	.	terminator	1791	1919	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K329051
BBa_K329057	.	terminator	1791	1870	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K329057	.	stem_loop	1802	1845	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K329057	.	engineered_region	1791	1870	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K329057	.	terminator	1879	1919	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K329057	.	engineered_region	1879	1919	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K329057	.	stop_codon	1912	1912	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K329057	.	polyA_site	1906	1919	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K329057	.	stem_loop	1886	1905	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K329057	.	engineered_region	816	1782	.	+	0	ID=BBa_P1003;Name=BBa_P1003;Parent=BBa_K329051
BBa_K329057	.	CDS	964	1782	.	+	0	ID=annotation1897112;Name=kanamycin resistance;Parent=BBa_P1003
BBa_K329057	.	ribosome_entry_site	419	430	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K329051
BBa_K329057	.	conserved_region	423	426	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K329057	.	CDS	437	670	.	+	0	ID=BBa_I732006;Name=BBa_I732006;Parent=BBa_K329051
BBa_K329057	.	stop_codon	665	670	.	+	0	ID=annotation1936906;Name=STOP;Parent=BBa_I732006
BBa_K329057	.	start_codon	437	439	.	+	0	ID=annotation1936905;Name=START;Parent=BBa_I732006
BBa_K329057	.	CDS	437	670	.	+	0	ID=annotation1936904;Name=lacZ;Parent=BBa_I732006
BBa_K329057	.	sequence_feature	44	367	.	+	0	ID=BBa_K329045;Name=BBa_K329045
BBa_K329057	.	sequence_feature	172	361	.	+	0	ID=annotation2114102;Name=mutated right arm of Tn916;Parent=BBa_K329045
BBa_K329057	.	sequence_feature	44	171	.	+	0	ID=annotation2114120;Name=mutated attR HK022;Parent=BBa_K329045
BBa_K329057	.	sequence_feature	1928	2235	.	+	0	ID=BBa_K329050;Name=BBa_K329050
BBa_K329057	.	sequence_feature	2178	2183	.	+	0	ID=annotation2113838;Name=Coupling;Parent=BBa_K329050
BBa_K329057	.	sequence_feature	2184	2189	.	+	0	ID=annotation2113839;Name=Mutation;Parent=BBa_K329050
BBa_K329057	.	sequence_feature	1928	2177	.	+	0	ID=annotation2113837;Name=Left arm HK022 MUT;Parent=BBa_K329050
BBa_K329057	.	sequence_feature	2190	2235	.	+	0	ID=annotation2113840;Name=Half of attL HK022 MUT;Parent=BBa_K329050
BBa_K329057	.	promoter	1	35	.	+	0	ID=BBa_J23119;Name=BBa_J23119
>BBa_K329057
ttgacagctagctcagtcctaggtataatgctagctactagagctagagaggtcactaataccatctaagtagttgatt
catagtgactgcatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatac
cttttttattttaaaaatagcataaaaatctagttatccgcataaaaactggacttatcacactttatcaaggtcaaaa
ccactcaatttactactaatttactacttatgaatgagctttgatacgacgatttatccttgaaaagtgaagatataaa
gatacttccaataaaatttgaatatttaataggtagacacttcaatactagtactagagtttacggctagctcagtcct
aggtacaatgctagctactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttaca
acgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaat
agcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccgg
caccagaagcggtgccggaaagctggctggagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagagctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtc
tcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagt
aatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctg
atttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccga
tgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactgg
ctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcga
ttcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgtt
cctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaa
tcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctgga
aagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttt
tgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctt
tggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatga
ataaattgcagtttcatttgatgctcgatgagtttttctaataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagctagagtgcaacattcgattctgcaatggcagaaatgaaacgcttgaa
taaagagaagcaacaggagcgtcttgttgcttagtagtacaaatgaatttactacttatttaccacttctgacagctaa
gacatccgttgaagcctgcttttttatactaacttgagcgaaacgaaaaatactcgaaattctacccgtagaatgcgta
ctggtaatgcaaactaaataaaaagataattagaaatttatactttgtttattttaaaaatccaacttattcacctaaa
gtgcaccgaccgtgaattactag