LOCUS       BBa_K329059             1632 bp    DNA     linear       22-Dec-2024
DEFINITION  [KanR] in between WT Tn916 Arms
ACCESSION   BBa_K329059
VERSION     BBa_K329059.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    80..95
                     /label=Rigth Xis binding site
     misc_feature    44..233
                     /label=BBa_K329041
     CDS             390..1208
                     /label=kanamycin resistance
     misc_feature    242..1208
                     /label=BBa_P1003
     stem_loop       1228..1271
                     /label=stem_loop
     misc_feature    1217..1296
                     /label=BBa_B0010
     terminator      1217..1296
                     /label=BBa_B0010
     stem_loop       1312..1331
                     /label=T7 TE
     misc_feature    1338..1338
                     /label=stop
     polyA_site      1332..1345
                     /label=polya
     misc_feature    1305..1345
                     /label=BBa_B0012
     terminator      1305..1345
                     /label=BBa_B0012
     terminator      1217..1345
                     /label=BBa_B0015
     misc_feature    1377..1382
                     /label=Coupling sequence
     misc_feature    1476..1490
                     /label=Left Xis binding site
     misc_feature    1354..1632
                     /label=BBa_K329046
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagttgaagt gtctacctat
       61 taaatattca aattttattg gaagtatctt tatatcttca cttttcaagg ataaatcgtc
      121 gtatcaaagc tcattcataa gtagtaaatt agtagtaaat tgagtggttt tgaccttgat
      181 aaagtgtgat aagtccagtt tttatgcgga taactagatt tttatgctat ttttactaga
      241 gctgatcctt caactcagca aaagttcgat ttattcaaca aagccacgtt gtgtctcaaa
      301 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
      361 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct
      421 cccgtccgcg cttaaactcc aacatggacg ctgatttata tgggtataaa tgggctcgcg
      481 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
      541 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtcc
      601 gtctcaactg gctgacggag tttatgcctc tcccgaccat caagcatttt atccgtactc
      661 ctgatgatgc gtggttactc accaccgcga ttcctgggaa aacagccttc caggtattag
      721 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggccgtgttc ctgcgccggt
      781 tacattcgat tcctgtttgt aattgtcctt ttaacagcga tcgtgtattt cgtcttgctc
      841 aggcgcaatc acgcatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
      901 atggctggcc tgttgaacaa gtctggaaag aaatgcacaa gctcttgcca ttctcaccgg
      961 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     1021 taataggttg tattgatgtt ggacgggtcg gaatcgcaga ccgttaccag gaccttgcca
     1081 ttctttggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     1141 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     1201 tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1261 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1321 tcgggtgggc ctttctgcgt ttatatacta gagactatga agaaaaaaat gatttttaaa
     1381 ataaacaaag tataaatttc taattatctt tttatatttt cttaaatgct cgtaaagcct
     1441 tattctatgt gctttcgagt atttttactg taggaagata cttcacgttt ctttgcatat
     1501 ttcctcatgt cttagctgtc agaagtggta aataagtagt aaattcattt gtactactaa
     1561 gcaacaagac gctcctgttg cttctcttta ttcaagcgtt tcatttctgc cattgcagaa
     1621 tcgaatgttg ca
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