BBa_K329048 1 BBa_K329048 Left arm Tn916 - Mutant + Mutant Lambda 2010-10-24T11:00:00Z 2015-05-08T01:12:04Z to write to write false false _449_ 0 5926 9 It's complicated false to write false Xavier Duportet annotation2113976 1 Half of attL lambda MUT range2113976 1 265 297 annotation2113972 1 Mutation range2113972 1 257 264 annotation2113971 1 Coupling range2113971 1 251 256 annotation2113970 1 Left arm Tn916 MUT range2113970 1 1 250 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K329043 1 BBa_K329043 Right arm Tn916 - Mutant + Mutant Lambda 2010-10-24T11:00:00Z 2015-05-08T01:12:04Z to write to write false true _449_ 0 5926 9 It's complicated false to write false Xavier Duportet annotation2114064 1 mutated attR lambda range2114064 1 1 128 annotation2114028 1 mutated right arm of tn916 range2114028 1 129 318 BBa_P1003 1 kanR kanamycin resistance cassette 2006-01-23T12:00:00Z 2015-05-08T01:14:11Z Kanamycin resistance cassette for modular BioBricks vector construction. false false _41_6_ 0 126 45 In stock false true Reshma Shetty annotation1897112 1 kanamycin resistance range1897112 1 149 967 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K329060 1 BBa_K329060 [KanR] in between MUT_MUT[Lambda] Tn916 Arms 2010-10-24T11:00:00Z 2015-05-08T01:12:04Z to write to write false false _449_ 0 5926 9 It's complicated false to write false Xavier Duportet component2114090 1 BBa_B0015 component2114083 1 BBa_P1003 component2114081 1 BBa_K329043 component2114079 1 BBa_J23119 component2114095 1 BBa_K329048 annotation2114090 1 BBa_B0015 range2114090 1 1351 1479 annotation2114081 1 BBa_K329043 range2114081 1 44 367 annotation2114079 1 BBa_J23119 range2114079 1 1 35 annotation2114095 1 BBa_K329048 range2114095 1 1488 1784 annotation2114083 1 BBa_P1003 range2114083 1 376 1342 BBa_K329060_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagctagagaggtcactaataccatctaagtagttgattcatagtgactgcatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgataccttttttattttaaaaatagcataaaaatctagttatccgcataaaaactggacttatcacactttatcaaggtcaaaaccactcaatttactactaatttactacttatgaatgagctttgatacgacgatttatccttgaaaagtgaagatataaagatacttccaataaaatttgaatatttaataggtagacacttcaatactagtactagagctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtgcaacattcgattctgcaatggcagaaatgaaacgcttgaataaagagaagcaacaggagcgtcttgttgcttagtagtacaaatgaatttactacttatttaccacttctgacagctaaattccgctttgcgactcaacctttttcacctaaagtgcaccgaccgtgaatactcgaaattctacccgtagaatgcgtactggtaatgcaaactaaataaaaagataattagaaatttatactttgtttattttaaaaatcatgttcagcttttttatactaacttgagcgaaacg BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K329048_sequence 1 tgcaacattcgattctgcaatggcagaaatgaaacgcttgaataaagagaagcaacaggagcgtcttgttgcttagtagtacaaatgaatttactacttatttaccacttctgacagctaaattccgctttgcgactcaacctttttcacctaaagtgcaccgaccgtgaatactcgaaattctacccgtagaatgcgtactggtaatgcaaactaaataaaaagataattagaaatttatactttgtttattttaaaaatcatgttcagcttttttatactaacttgagcgaaacg BBa_P1003_sequence 1 ctgatccttcaactcagcaaaagttcgatttattcaacaaagccacgttgtgtctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K329043_sequence 1 ctagagaggtcactaataccatctaagtagttgattcatagtgactgcatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgataccttttttattttaaaaatagcataaaaatctagttatccgcataaaaactggacttatcacactttatcaaggtcaaaaccactcaatttactactaatttactacttatgaatgagctttgatacgacgatttatccttgaaaagtgaagatataaagatacttccaataaaatttgaatatttaataggtagacacttcaatactag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z