LOCUS       BBa_K329061             1729 bp    DNA     linear       26-Dec-2024
DEFINITION  [KanR] in between WT_MUT[HK022] Tn916 Arms
ACCESSION   BBa_K329061
VERSION     BBa_K329061.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    118..307
                     /label=BBa_K329041
     misc_feature    44..117
                     /label=half of attR HK022
     misc_feature    44..313
                     /label=BBa_K329044
     CDS             470..1288
                     /label=kanamycin resistance
     misc_feature    322..1288
                     /label=BBa_P1003
     stem_loop       1308..1351
                     /label=stem_loop
     misc_feature    1297..1376
                     /label=BBa_B0010
     terminator      1297..1376
                     /label=BBa_B0010
     stem_loop       1392..1411
                     /label=T7 TE
     misc_feature    1418..1418
                     /label=stop
     polyA_site      1412..1425
                     /label=polya
     misc_feature    1385..1425
                     /label=BBa_B0012
     terminator      1385..1425
                     /label=BBa_B0012
     terminator      1297..1425
                     /label=BBa_B0015
     misc_feature    1434..1683
                     /label=BBa_K329046
     misc_feature    1684..1689
                     /label=Coupling
     misc_feature    1690..1695
                     /label=Mutation
     misc_feature    1696..1729
                     /label=Half of attL HK022 MUT
     misc_feature    1434..1729
                     /label=BBa_K329049
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagctagaga caaatgattt
       61 tattttgact aataatgacc tacttacatt aatttactga taaccttttt tattttaaaa
      121 atagcataaa aatctagtta tccgcataaa aactggactt atcacacttt atcaaggtca
      181 aaaccactca atttactact aatttactac ttatgaatga gctttgatac gacgatttat
      241 ccttgaaaag tgaagatata aagatacttc caataaaatt tgaatattta ataggtagac
      301 acttcaatac tagtactaga gctgatcctt caactcagca aaagttcgat ttattcaaca
      361 aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
      421 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
      481 tcaacgggaa acgtcttgct cccgtccgcg cttaaactcc aacatggacg ctgatttata
      541 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
      601 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
      661 tgttacagat gagatggtcc gtctcaactg gctgacggag tttatgcctc tcccgaccat
      721 caagcatttt atccgtactc ctgatgatgc gtggttactc accaccgcga ttcctgggaa
      781 aacagccttc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
      841 ggccgtgttc ctgcgccggt tacattcgat tcctgtttgt aattgtcctt ttaacagcga
      901 tcgtgtattt cgtcttgctc aggcgcaatc acgcatgaat aacggtttgg ttgatgcgag
      961 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcacaa
     1021 gctcttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     1081 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgggtcg gaatcgcaga
     1141 ccgttaccag gaccttgcca ttctttggaa ctgcctcggt gagttttctc cttcattaca
     1201 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     1261 tttgatgctc gatgagtttt tctaataata ctagagccag gcatcaaata aaacgaaagg
     1321 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1381 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtgcaaca
     1441 ttcgattctg caatggcaga aatgaaacgc ttgaataaag agaagcaaca ggagcgtctt
     1501 gttgcttagt agtacaaatg aatttactac ttatttacca cttctgacag ctaagacatg
     1561 aggaaatatg caaagaaacg tgaagtatct tcctacagta aaaatactcg aaagcacata
     1621 gaataaggct ttacgagcat ttaagaaaat ataaaaagat aattagaaat ttatactttg
     1681 tttattttaa aaatccaact tattcaccta aagtgcaccg accgtgaat
//