LOCUS       BBa_K331000              831 bp    DNA     linear       14-Nov-2024
DEFINITION  IPTG inducible Lumazine Synthase
ACCESSION   BBa_K331000
VERSION     BBa_K331000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..694
                     /label=BBa_K249002
     stem_loop       714..757
                     /label=stem_loop
     misc_feature    703..782
                     /label=BBa_B0010
     terminator      703..782
                     /label=BBa_B0010
     stem_loop       798..817
                     /label=T7 TE
     misc_feature    824..824
                     /label=stop
     polyA_site      818..831
                     /label=polya
     misc_feature    791..831
                     /label=BBa_B0012
     terminator      791..831
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcagattta
      241 tgaaggcaaa ctgaccgcgg aaggcctgcg ctttggcatt gtggcgagcc gctttaacca
      301 tgcgctggtg gatcgcctgg tggaaggcgc gattgattgc attgtgcgcc atggtggtcg
      361 cgaagaagat attaccctgg tgcgcgtgcc gggcagctgg gaaattccgg tggcggcggg
      421 cgaactggcg cgcaaagaag atattgatgc ggtgattgcg attggcgtgc tgattgaagg
      481 cgcggaaccg cattttgatt atattgcgag cgaagtgagc aaaggcctgg cgaacctgag
      541 cctggaactg cgcaaaccga ttacctttgg cgtgattacc gcggatgaac tggaagaagc
      601 gattgaacgc gcgggcacca aacatggcaa caaaggctgg gaagcggcgc tgagcgcgat
      661 tgaaatggcg aacctgttta aaagcctgcg ctagtactag agccaggcat caaataaaac
      721 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
      781 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//