LOCUS BBa_K331003 1125 bp DNA linear 14-Nov-2024 DEFINITION IPTG Inducible ECFP with N-terminal Arginine Tag ACCESSION BBa_K331003 VERSION BBa_K331003.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 misc_feature 227..229 /label=start misc_feature 230..268 /label=Arginine Tag misc_feature 983..988 /label=double stop misc_feature 227..988 /label=BBa_K331001 stem_loop 1008..1051 /label=stem_loop misc_feature 997..1076 /label=BBa_B0010 terminator 997..1076 /label=BBa_B0010 stem_loop 1092..1111 /label=T7 TE misc_feature 1118..1118 /label=stop polyA_site 1112..1125 /label=polya misc_feature 1085..1125 /label=BBa_B0012 terminator 1085..1125 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gccgccgccg 241 ccgccgccgc cgccgccgca ctagaatggt gagcaagggc gaggagctgt tcaccggggt 301 ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca gcgtgtccgg 361 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 421 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt 481 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 541 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 601 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 661 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta 721 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat 781 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 841 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc 901 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 961 cggcatggac gagctgtaca agtaataata ctagagccag gcatcaaata aaacgaaagg 1021 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1081 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //