LOCUS       BBa_K331004             1119 bp    DNA     linear       31-Dec-2025
DEFINITION  IPTG Inducible ECFP with C-terminal Arginine Tag
ACCESSION   BBa_K331004
VERSION     BBa_K331004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=start
     misc_feature    980..982
                     /label=stop
     misc_feature    951..979
                     /label=Arginine Tag
     misc_feature    227..982
                     /label=BBa_K331002
     stem_loop       1002..1045
                     /label=stem_loop
     misc_feature    991..1070
                     /label=BBa_B0010
     terminator      991..1070
                     /label=BBa_B0010
     stem_loop       1086..1105
                     /label=T7 TE
     misc_feature    1112..1112
                     /label=stop
     polyA_site      1106..1119
                     /label=polya
     misc_feature    1079..1119
                     /label=BBa_B0012
     terminator      1079..1119
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg tgagcaaggg
      241 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg
      301 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      361 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      421 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      481 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      541 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      601 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      661 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
      721 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      781 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      841 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      901 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagcactaga gccgccgccg
      961 ccgccgccgc cgccgccgct aatactagag ccaggcatca aataaaacga aaggctcagt
     1021 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     1081 acactggctc accttcgggt gggcctttct gcgtttata
//