LOCUS       BBa_K331005             2753 bp    DNA     linear       23-May-2024
DEFINITION  Arabinose Repressed ECFP and EYFP with N-terminal Arginine Tag
ACCESSION   BBa_K331005
VERSION     BBa_K331005.1  
FEATURES             Location/Qualifiers
     promoter        1..130
                     /label=BBa_I13453
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     CDS             160..776
                     /label=tetR
     misc_feature    777..810
                     /label=SsrA
     misc_feature    817..841
                     /label=Help:Barcodes
     CDS             157..816
                     /label=BBa_C0040
     stem_loop       861..904
                     /label=stem_loop
     misc_feature    850..929
                     /label=BBa_B0010
     terminator      850..929
                     /label=BBa_B0010
     stem_loop       945..964
                     /label=T7 TE
     misc_feature    971..971
                     /label=stop
     polyA_site      965..978
                     /label=polya
     misc_feature    938..978
                     /label=BBa_B0012
     terminator      938..978
                     /label=BBa_B0012
     misc_feature    987..1005
                     /label=TetR 1
     misc_feature    987..1040
                     /label=BBa_R0040
     promoter        1006..1011
                     /label=-35
     misc_feature    1012..1030
                     /label=TetR 2
     promoter        1029..1034
                     /label=-10
     promoter        987..1040
                     /label=BBa_R0040
     misc_feature    139..1040
                     /label=BBa_Q04400
     misc_feature    1..1040
                     /label=BBa_K249001
     misc_feature    1053..1056
                     /label=conserved
     RBS             1049..1060
                     /label=BBa_B0034
     misc_feature    1067..1069
                     /label=start
     misc_feature    1070..1108
                     /label=Arginine Tag
     misc_feature    1823..1828
                     /label=double stop
     misc_feature    1067..1828
                     /label=BBa_K331001
     misc_feature    1841..1844
                     /label=conserved
     RBS             1837..1848
                     /label=BBa_B0034
     misc_feature    1855..2616
                     /label=BBa_K249008
     stem_loop       2636..2679
                     /label=stem_loop
     misc_feature    2625..2704
                     /label=BBa_B0010
     terminator      2625..2704
                     /label=BBa_B0010
     stem_loop       2720..2739
                     /label=T7 TE
     misc_feature    2746..2746
                     /label=stop
     polyA_site      2740..2753
                     /label=polya
     misc_feature    2713..2753
                     /label=BBa_B0012
     terminator      2713..2753
                     /label=BBa_B0012
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa
      181 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt
      241 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag
      301 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc
      361 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt
      421 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa
      481 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag
      541 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat
      601 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca
      661 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc
      721 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct
      781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca
      841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      961 ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca
     1021 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgc gccgccgccg
     1081 ccgccgccgc cgccgccgca ctagaatggt gagcaagggc gaggagctgt tcaccggggt
     1141 ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca gcgtgtccgg
     1201 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1261 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt
     1321 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1381 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1441 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1501 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta
     1561 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1621 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1681 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     1741 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1801 cggcatggac gagctgtaca agtaataata ctagagaaag aggagaaata ctagatgcgc
     1861 cgccgccgcc gccgccgccg ccgccgcact agaatggtga gcaagggcga ggagctgttc
     1921 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1981 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2041 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     2101 caatgcttcg cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg
     2161 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     2221 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     2281 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     2341 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     2401 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     2461 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     2521 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     2581 atcactctcg gcatggacga gctgtacaag taataatact agagccaggc atcaaataaa
     2641 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2701 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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