##gff-version 3
##sequence-region BBa_K331005 1 2753
BBa_K331005	.	engineered_region	1855	2616	.	+	0	ID=BBa_K249008;Name=BBa_K249008
BBa_K331005	.	terminator	2625	2704	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K331005	.	engineered_region	2625	2704	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K331005	.	stem_loop	2636	2679	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K331005	.	engineered_region	1067	1828	.	+	0	ID=BBa_K331001;Name=BBa_K331001
BBa_K331005	.	sequence_feature	1070	1108	.	+	0	ID=annotation2071683;Name=Arginine Tag;Parent=BBa_K331001
BBa_K331005	.	stop_codon	1823	1828	.	+	0	ID=annotation2071684;Name=double stop;Parent=BBa_K331001
BBa_K331005	.	start_codon	1067	1069	.	+	0	ID=annotation2071682;Name=start;Parent=BBa_K331001
BBa_K331005	.	engineered_region	1	1040	.	+	0	ID=BBa_K249001;Name=BBa_K249001
BBa_K331005	.	engineered_region	139	1040	.	+	0	ID=BBa_Q04400;Name=BBa_Q04400;Parent=BBa_K249001
BBa_K331005	.	terminator	938	978	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_Q04400
BBa_K331005	.	engineered_region	938	978	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K331005	.	stem_loop	945	964	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K331005	.	polyA_site	965	978	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K331005	.	stop_codon	971	971	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K331005	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_Q04400
BBa_K331005	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K331005	.	promoter	987	1040	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04400
BBa_K331005	.	promoter	1006	1011	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K331005	.	non_covalent_binding_site	1012	1030	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K331005	.	engineered_region	987	1040	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K331005	.	non_covalent_binding_site	987	1005	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K331005	.	promoter	1029	1034	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K331005	.	CDS	157	816	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04400
BBa_K331005	.	CDS	160	776	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K331005	.	engineered_tag	817	841	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K331005	.	tag	777	810	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K331005	.	terminator	850	929	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_Q04400
BBa_K331005	.	engineered_region	850	929	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K331005	.	stem_loop	861	904	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K331005	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K249001
BBa_K331005	.	ribosome_entry_site	1049	1060	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K331005	.	conserved_region	1053	1056	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K331005	.	ribosome_entry_site	1837	1848	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K331005	.	conserved_region	1841	1844	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K331005	.	terminator	2713	2753	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K331005	.	engineered_region	2713	2753	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K331005	.	stem_loop	2720	2739	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K331005	.	polyA_site	2740	2753	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K331005	.	stop_codon	2746	2746	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
>BBa_K331005
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacc
cgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacg
ccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacg
taataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctaca
gaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatg
cactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaaggga
aacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagcc
ttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacg
aaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatag
agatactgagcactactagagaaagaggagaaatactagatgcgccgccgccgccgccgccgccgccgccgcactagaa
tggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaa
gttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaag
ctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatga
agcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaa
ctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaag
gaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcaga
agaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagca
gaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagac
cccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgt
acaagtaataatactagagaaagaggagaaatactagatgcgccgccgccgccgccgccgccgccgccgcactagaatg
gtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagt
tcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagct
gcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaag
ctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaact
acaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaagga
ggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaag
aacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcaga
acacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccc
caacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtac
aagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgt
ttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata