LOCUS BBa_K331006 2741 bp DNA linear 22-Nov-2024 DEFINITION Arabinose Repressed ECFP and EYFP with C-terminal Arginine Tag ACCESSION BBa_K331006 VERSION BBa_K331006.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 CDS 160..776 /label=tetR misc_feature 777..810 /label=SsrA misc_feature 817..841 /label=Help:Barcodes CDS 157..816 /label=BBa_C0040 stem_loop 861..904 /label=stem_loop misc_feature 850..929 /label=BBa_B0010 terminator 850..929 /label=BBa_B0010 stem_loop 945..964 /label=T7 TE misc_feature 971..971 /label=stop polyA_site 965..978 /label=polya misc_feature 938..978 /label=BBa_B0012 terminator 938..978 /label=BBa_B0012 misc_feature 987..1005 /label=TetR 1 misc_feature 987..1040 /label=BBa_R0040 promoter 1006..1011 /label=-35 misc_feature 1012..1030 /label=TetR 2 promoter 1029..1034 /label=-10 promoter 987..1040 /label=BBa_R0040 misc_feature 139..1040 /label=BBa_Q04400 misc_feature 1..1040 /label=BBa_K249001 misc_feature 1053..1056 /label=conserved RBS 1049..1060 /label=BBa_B0034 misc_feature 1067..1069 /label=start misc_feature 1820..1822 /label=stop misc_feature 1791..1819 /label=Arginine Tag misc_feature 1067..1822 /label=BBa_K331002 misc_feature 1835..1838 /label=conserved RBS 1831..1842 /label=BBa_B0034 misc_feature 1849..2604 /label=BBa_K249006 stem_loop 2624..2667 /label=stem_loop misc_feature 2613..2692 /label=BBa_B0010 terminator 2613..2692 /label=BBa_B0010 stem_loop 2708..2727 /label=T7 TE misc_feature 2734..2734 /label=stop polyA_site 2728..2741 /label=polya misc_feature 2701..2741 /label=BBa_B0012 terminator 2701..2741 /label=BBa_B0012 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatgt ccagattaga taaaagtaaa 181 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt 241 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag 301 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc 361 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt 421 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa 481 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag 541 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat 601 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca 661 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc 721 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct 781 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 841 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 901 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 961 ggcctttctg cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca 1021 gtgatagaga tactgagcac tactagagaa agaggagaaa tactagatgg tgagcaaggg 1081 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtgaacgg 1141 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct 1201 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct 1261 gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt 1321 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg 1381 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga 1441 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa 1501 ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa 1561 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca 1621 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca 1681 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt 1741 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagcactaga gccgccgccg 1801 ccgccgccgc cgccgccgct aatactagag aaagaggaga aatactagat ggtgagcaag 1861 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 1921 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 1981 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 2041 ttcggctacg gcctgcaatg cttcgcccgc taccccgacc acatgaagct gcacgacttc 2101 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 2161 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 2221 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 2281 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg 2341 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 2401 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac 2461 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 2521 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagcacta gagccgccgc 2581 cgccgccgcc gccgccgccg ctaatactag agccaggcat caaataaaac gaaaggctca 2641 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 2701 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //