LOCUS       BBa_K331014             1311 bp    DNA     linear       14-Nov-2024
DEFINITION  IPTG Inducible Catechol 1,2-dioxygenase with N-terminal Arginine 
            Tag
ACCESSION   BBa_K331014
VERSION     BBa_K331014.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..1174
                     /label=BBa_K331008
     stem_loop       1194..1237
                     /label=stem_loop
     misc_feature    1183..1262
                     /label=BBa_B0010
     terminator      1183..1262
                     /label=BBa_B0010
     stem_loop       1278..1297
                     /label=T7 TE
     misc_feature    1304..1304
                     /label=stop
     polyA_site      1298..1311
                     /label=polya
     misc_feature    1271..1311
                     /label=BBa_B0012
     terminator      1271..1311
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gccgccgccg
      241 ccgccgccgc cgccgccgca ctagaaccgt gaacattagc cataccgcgg aagtgcagca
      301 gttttttgaa caggcggcgg gctttggcaa cgcggcgggc aacccgcgtc tgaaacgtat
      361 tgtgcagcgt ctgctgcaag ataccgcgcg tctgattgaa gatctggata ttagcgaaga
      421 tgaattttgg catgcggtgg attatctgaa ccgtctgggc ggccgtggcg aagcgggcct
      481 gctggtggcg ggcctgggca ttgaacattt tctggatctg ctgcaagatg cgaaagatca
      541 ggaagcgggc cgtgtgggcg gcaccccgcg taccattgaa ggcccgctgt atgtggcggg
      601 cgcgccgatt gcgcagggcg aagtgcgtat ggatgatggc agcgaagaag gcgtggcgac
      661 cgtgatgttt ctggaaggcc aggtgctgga tccgcatggc catccgctgc cgggcgcgac
      721 cgtggatctg tggcatgcga acacccgtgg cacctatagc ttttttgatc cgagccagag
      781 cgcgtataac ctgcgtcgtc gtattgtgac cgatgcgcag ggccgttatc gtgcgcgtag
      841 cattgtgccg agcggctatg gctgcgatcc gcagggcccg acccaggaat gcctggatct
      901 gctgggccgt catggccagc gtccggcgca tgtgcatttt tttattagcg cgccgggcta
      961 tcgtcatctg accacccaga ttaacctgag cggcgataaa tatctgtggg atgattttgc
     1021 gtatgcgacc cgtgatggcc tggtgggcga agtggtgttt gtggaaggcc cggaaggccg
     1081 tcatgcggaa ctgaaatttg attttcagct gcaacaggcg cagggcggcg cggatgaaca
     1141 gcgtagcggc cgtccgcgtg cgctgcaaga agcgtactag agccaggcat caaataaaac
     1201 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1261 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//