BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961224
1
-35
range1961224
1
137
142
annotation1961227
1
start
range1961227
1
173
173
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961222
1
BBa_R0010
range1961222
1
1
200
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K331014
1
BBa_K331014
IPTG Inducible Catechol 1,2-dioxygenase with N-terminal Arginine Tag
2010-06-30T11:00:00Z
2015-05-08T01:12:04Z
As in part K331008
This part is IPTG inducible to produce catechol 1,2-dioxygenase with an N-terminal arginine tag. The arginine sequence allows for the targeting of ECFP into the Lumazine Synthase microcompatment (Bba_K249003).
true
false
_463_
0
4444
9
Discontinued
false
None
false
Lisza Bruder
component2072151
1
BBa_B0010
component2072153
1
BBa_B0012
component2072150
1
BBa_K331008
component2072139
1
BBa_R0010
component2072147
1
BBa_B0034
annotation2072153
1
BBa_B0012
range2072153
1
1271
1311
annotation2072150
1
BBa_K331008
range2072150
1
227
1174
annotation2072147
1
BBa_B0034
range2072147
1
209
220
annotation2072151
1
BBa_B0010
range2072151
1
1183
1262
annotation2072139
1
BBa_R0010
range2072139
1
1
200
BBa_K331008
1
BBa_K331008
Outer Membrane Protein OmpA
2010-06-27T11:00:00Z
2015-05-08T01:12:04Z
As in part BBa_K331006
Catechol 1,2-dioxygenase from part (BBa_K331006) with an arginine tag which will allow for targeting into the lumazine Synthase microcompatment (BBa_K331000)
false
false
_463_
0
4444
9
In stock
false
Removed start codon
false
Lisza Bruder
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K331008_sequence
1
atgaaaaaaaccgcgattgcgctggcggtggcgctggcgggctttgcgaccgtggcgcaggcg
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K331014_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcgccgccgccgccgccgccgccgccgccgcactagaaccgtgaacattagccataccgcggaagtgcagcagttttttgaacaggcggcgggctttggcaacgcggcgggcaacccgcgtctgaaacgtattgtgcagcgtctgctgcaagataccgcgcgtctgattgaagatctggatattagcgaagatgaattttggcatgcggtggattatctgaaccgtctgggcggccgtggcgaagcgggcctgctggtggcgggcctgggcattgaacattttctggatctgctgcaagatgcgaaagatcaggaagcgggccgtgtgggcggcaccccgcgtaccattgaaggcccgctgtatgtggcgggcgcgccgattgcgcagggcgaagtgcgtatggatgatggcagcgaagaaggcgtggcgaccgtgatgtttctggaaggccaggtgctggatccgcatggccatccgctgccgggcgcgaccgtggatctgtggcatgcgaacacccgtggcacctatagcttttttgatccgagccagagcgcgtataacctgcgtcgtcgtattgtgaccgatgcgcagggccgttatcgtgcgcgtagcattgtgccgagcggctatggctgcgatccgcagggcccgacccaggaatgcctggatctgctgggccgtcatggccagcgtccggcgcatgtgcatttttttattagcgcgccgggctatcgtcatctgaccacccagattaacctgagcggcgataaatatctgtgggatgattttgcgtatgcgacccgtgatggcctggtgggcgaagtggtgtttgtggaaggcccggaaggccgtcatgcggaactgaaatttgattttcagctgcaacaggcgcagggcggcgcggatgaacagcgtagcggccgtccgcgtgcgctgcaagaagcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z