LOCUS       BBa_K331015             1314 bp    DNA     linear       05-Nov-2024
DEFINITION  IPTG Inducible Catechol 1,2-dioxygenase with C-terminal Arginine 
            Tag
ACCESSION   BBa_K331015
VERSION     BBa_K331015.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..1177
                     /label=BBa_K331009
     stem_loop       1197..1240
                     /label=stem_loop
     misc_feature    1186..1265
                     /label=BBa_B0010
     terminator      1186..1265
                     /label=BBa_B0010
     stem_loop       1281..1300
                     /label=T7 TE
     misc_feature    1307..1307
                     /label=stop
     polyA_site      1301..1314
                     /label=polya
     misc_feature    1274..1314
                     /label=BBa_B0012
     terminator      1274..1314
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ccgtgaacat
      241 tagccatacc gcggaagtgc agcagttttt tgaacaggcg gcgggctttg gcaacgcggc
      301 gggcaacccg cgtctgaaac gtattgtgca gcgtctgctg caagataccg cgcgtctgat
      361 tgaagatctg gatattagcg aagatgaatt ttggcatgcg gtggattatc tgaaccgtct
      421 gggcggccgt ggcgaagcgg gcctgctggt ggcgggcctg ggcattgaac attttctgga
      481 tctgctgcaa gatgcgaaag atcaggaagc gggccgtgtg ggcggcaccc cgcgtaccat
      541 tgaaggcccg ctgtatgtgg cgggcgcgcc gattgcgcag ggcgaagtgc gtatggatga
      601 tggcagcgaa gaaggcgtgg cgaccgtgat gtttctggaa ggccaggtgc tggatccgca
      661 tggccatccg ctgccgggcg cgaccgtgga tctgtggcat gcgaacaccc gtggcaccta
      721 tagctttttt gatccgagcc agagcgcgta taacctgcgt cgtcgtattg tgaccgatgc
      781 gcagggccgt tatcgtgcgc gtagcattgt gccgagcggc tatggctgcg atccgcaggg
      841 cccgacccag gaatgcctgg atctgctggg ccgtcatggc cagcgtccgg cgcatgtgca
      901 tttttttatt agcgcgccgg gctatcgtca tctgaccacc cagattaacc tgagcggcga
      961 taaatatctg tgggatgatt ttgcgtatgc gacccgtgat ggcctggtgg gcgaagtggt
     1021 gtttgtggaa ggcccggaag gccgtcatgc ggaactgaaa tttgattttc agctgcaaca
     1081 ggcgcagggc ggcgcggatg aacagcgtag cggccgtccg cgtgcgctgc aagaagcgac
     1141 tagacgccgc cgccgccgcc gccgccgccg ccgctaatac tagagccagg catcaaataa
     1201 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     1261 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//