LOCUS       BBa_K331016             1323 bp    DNA     linear       26-Dec-2024
DEFINITION  IPTG Inducible Catechol 2,3-dioxygenase with N-terminal Arginine 
            Tag
ACCESSION   BBa_K331016
VERSION     BBa_K331016.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1186
                     /label=BBa_K331010
     stem_loop       1206..1249
                     /label=stem_loop
     misc_feature    1195..1274
                     /label=BBa_B0010
     terminator      1195..1274
                     /label=BBa_B0010
     stem_loop       1290..1309
                     /label=T7 TE
     misc_feature    1316..1316
                     /label=stop
     polyA_site      1310..1323
                     /label=polya
     misc_feature    1283..1323
                     /label=BBa_B0012
     terminator      1283..1323
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gccgccgccg
      241 ccgccgccgc cgccgccgca ctagaaacaa aggtgtaatg cgaccgggcc atgtgcagct
      301 gcgtgtactg gacatgagca aggccctgga acactacgtc gagttgctgg gcctgatcga
      361 gatggaccgt gacgaccagg gccgtgtcta tctgaaggct tggaccgaag tggataagtt
      421 ttccctggtg ctacgcgagg ctgacgagcc gggcatggat tttatgggtt tcaaggttgt
      481 ggatgaggat gctctccggc aactggagcg ggatctgatg gcatatggct gtgccgttga
      541 gcagctaccc gcaggtgaac tgaacagttg tggccggcgc gtgcgcttcc aggccccctc
      601 cgggcatcac ttcgagttgt atgcagacaa ggaatatact ggaaagtggg gtttgaatga
      661 cgtcaatccc gaggcatggc cgcgcgatct gaaaggtatg gcggctgtgc gtttcgacca
      721 cgccctcatg tatggcgacg aattgccggc gacctatgac ctgttcacca aggtgctcgg
      781 tttctatctg gccgaacagg tgctggacga aaatggcacg cgcgtcgccc agtttctcag
      841 tctgtcgacc aaggcccacg acgtggcctt cattcaccat ccggaaaaag gccgcctcca
      901 tcatgtgtcc ttccacctcg aaacctggga agacttgctt cgcgccgccg acctgatctc
      961 catgaccgac acatctatcg atatcggccc aacccgccac ggcctcactc acggcaagac
     1021 catctacttc ttcgacccgt ccggtaaccg caacgaagtg ttctgcgggg gagattacaa
     1081 ctacccggac cacaaaccgg tgacctggac caccgaccag ctgggcaagg cgatctttta
     1141 ccacgaccgc attctcaacg aacgattcat gaccgtgctg acctgatact agagccaggc
     1201 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1261 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1321 ata
//