LOCUS       BBa_K331017             1323 bp    DNA     linear       14-Nov-2024
DEFINITION  IPTG Inducible Catechol 2,3-dioxygenase with C-terminal Arginine 
            Tag
ACCESSION   BBa_K331017
VERSION     BBa_K331017.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1186
                     /label=BBa_K331011
     stem_loop       1206..1249
                     /label=stem_loop
     misc_feature    1195..1274
                     /label=BBa_B0010
     terminator      1195..1274
                     /label=BBa_B0010
     stem_loop       1290..1309
                     /label=T7 TE
     misc_feature    1316..1316
                     /label=stop
     polyA_site      1310..1323
                     /label=polya
     misc_feature    1283..1323
                     /label=BBa_B0012
     terminator      1283..1323
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga acaaaggtgt
      241 aatgcgaccg ggccatgtgc agctgcgtgt actggacatg agcaaggccc tggaacacta
      301 cgtcgagttg ctgggcctga tcgagatgga ccgtgacgac cagggccgtg tctatctgaa
      361 ggcttggacc gaagtggata agttttccct ggtgctacgc gaggctgacg agccgggcat
      421 ggattttatg ggtttcaagg ttgtggatga ggatgctctc cggcaactgg agcgggatct
      481 gatggcatat ggctgtgccg ttgagcagct acccgcaggt gaactgaaca gttgtggccg
      541 gcgcgtgcgc ttccaggccc cctccgggca tcacttcgag ttgtatgcag acaaggaata
      601 tactggaaag tggggtttga atgacgtcaa tcccgaggca tggccgcgcg atctgaaagg
      661 tatggcggct gtgcgtttcg accacgccct catgtatggc gacgaattgc cggcgaccta
      721 tgacctgttc accaaggtgc tcggtttcta tctggccgaa caggtgctgg acgaaaatgg
      781 cacgcgcgtc gcccagtttc tcagtctgtc gaccaaggcc cacgacgtgg ccttcattca
      841 ccatccggaa aaaggccgcc tccatcatgt gtccttccac ctcgaaacct gggaagactt
      901 gcttcgcgcc gccgacctga tctccatgac cgacacatct atcgatatcg gcccaacccg
      961 ccacggcctc actcacggca agaccatcta cttcttcgac ccgtccggta accgcaacga
     1021 agtgttctgc gggggagatt acaactaccc ggaccacaaa ccggtgacct ggaccaccga
     1081 ccagctgggc aaggcgatct tttaccacga ccgcattctc aacgaacgat tcatgaccgt
     1141 gctgaccact agacgccgcc gccgccgccg ccgccgccgc cgctaatact agagccaggc
     1201 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1261 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1321 ata
//