LOCUS BBa_K331019 3580 bp DNA linear 22-Nov-2024 DEFINITION Lumazine Synthase Microcompartment Test Construct (ECFP & EYFP with C-Terminal Arginine Tag) ACCESSION BBa_K331019 VERSION BBa_K331019.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 CDS 230..694 /label=BBa_K249002 stem_loop 714..757 /label=stem_loop misc_feature 703..782 /label=BBa_B0010 terminator 703..782 /label=BBa_B0010 stem_loop 798..817 /label=T7 TE misc_feature 824..824 /label=stop polyA_site 818..831 /label=polya misc_feature 791..831 /label=BBa_B0012 terminator 791..831 /label=BBa_B0012 misc_feature 1..831 /label=BBa_K331000 promoter 840..969 /label=BBa_I13453 misc_feature 982..985 /label=conserved RBS 978..989 /label=BBa_B0034 CDS 999..1615 /label=tetR misc_feature 1616..1649 /label=SsrA misc_feature 1656..1680 /label=Help:Barcodes CDS 996..1655 /label=BBa_C0040 stem_loop 1700..1743 /label=stem_loop misc_feature 1689..1768 /label=BBa_B0010 terminator 1689..1768 /label=BBa_B0010 stem_loop 1784..1803 /label=T7 TE misc_feature 1810..1810 /label=stop polyA_site 1804..1817 /label=polya misc_feature 1777..1817 /label=BBa_B0012 terminator 1777..1817 /label=BBa_B0012 misc_feature 1826..1844 /label=TetR 1 misc_feature 1826..1879 /label=BBa_R0040 promoter 1845..1850 /label=-35 misc_feature 1851..1869 /label=TetR 2 promoter 1868..1873 /label=-10 promoter 1826..1879 /label=BBa_R0040 misc_feature 978..1879 /label=BBa_Q04400 misc_feature 840..1879 /label=BBa_K249001 misc_feature 1892..1895 /label=conserved RBS 1888..1899 /label=BBa_B0034 misc_feature 1906..1908 /label=start misc_feature 2659..2661 /label=stop misc_feature 2630..2658 /label=Arginine Tag misc_feature 1906..2661 /label=BBa_K331002 misc_feature 2674..2677 /label=conserved RBS 2670..2681 /label=BBa_B0034 misc_feature 2688..3443 /label=BBa_K249006 stem_loop 3463..3506 /label=stem_loop misc_feature 3452..3531 /label=BBa_B0010 terminator 3452..3531 /label=BBa_B0010 stem_loop 3547..3566 /label=T7 TE misc_feature 3573..3573 /label=stop polyA_site 3567..3580 /label=polya misc_feature 3540..3580 /label=BBa_B0012 terminator 3540..3580 /label=BBa_B0012 misc_feature 840..3580 /label=BBa_K331006 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcagattta 241 tgaaggcaaa ctgaccgcgg aaggcctgcg ctttggcatt gtggcgagcc gctttaacca 301 tgcgctggtg gatcgcctgg tggaaggcgc gattgattgc attgtgcgcc atggtggtcg 361 cgaagaagat attaccctgg tgcgcgtgcc gggcagctgg gaaattccgg tggcggcggg 421 cgaactggcg cgcaaagaag atattgatgc ggtgattgcg attggcgtgc tgattgaagg 481 cgcggaaccg cattttgatt atattgcgag cgaagtgagc aaaggcctgg cgaacctgag 541 cctggaactg cgcaaaccga ttacctttgg cgtgattacc gcggatgaac tggaagaagc 601 gattgaacgc gcgggcacca aacatggcaa caaaggctgg gaagcggcgc tgagcgcgat 661 tgaaatggcg aacctgttta aaagcctgcg ctagtactag agccaggcat caaataaaac 721 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 781 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga 841 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 901 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat accgtttttt 961 tgggctagct actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag 1021 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta 1081 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc 1141 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc 1201 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg 1261 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac 1321 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga 1381 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc 1441 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat 1501 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg 1561 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg 1621 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac 1681 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt 1741 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg 1801 gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca tccctatcag 1861 tgatagagat actgagcact actagagaaa gaggagaaat actagatggt gagcaagggc 1921 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc 1981 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg 2041 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg 2101 acctggggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc 2161 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc 2221 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag 2281 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac 2341 tacatcagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac 2401 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag 2461 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag 2521 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg 2581 accgccgccg ggatcactct cggcatggac gagctgtaca agcactagag ccgccgccgc 2641 cgccgccgcc gccgccgcta atactagaga aagaggagaa atactagatg gtgagcaagg 2701 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 2761 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 2821 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct 2881 tcggctacgg cctgcaatgc ttcgcccgct accccgacca catgaagctg cacgacttct 2941 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 3001 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 3061 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 3121 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 3181 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 3241 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc 3301 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 3361 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagcactag agccgccgcc 3421 gccgccgccg ccgccgccgc taatactaga gccaggcatc aaataaaacg aaaggctcag 3481 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 3541 cacactggct caccttcggg tgggcctttc tgcgtttata //